Quantitative Phytochemical Screening and GC-MS Analysis of the Ethanolic Extract of Tabebuia roseo-alba (Ridl) Sand
DOI:
https://doi.org/10.21048/IJND.2021.58.3.27164Keywords:
Gas chromatography-mass spectrometry, secondary metabolites, medicinal plantsAbstract
Medicinal plants have been used in folkloric medicine to treat various ailments. The compound investigations of medicinal plants have largely been driven to discover new drugs to treat human diseases. The study was designed to estimate the amount of phytochemical constituents and also to explore the presence of bioactive compounds in ethanolic extract of Tabebuia roseo-alba using Gas Chromatography-Mass Spectrometry (GC-MS). Quantitative phytochemical analysis was carried out by standard procedures. GC-MS analysis of the plant extracts was performed by using GC SHIMADZU QP2010 system and Gas Chromatograph interfaced to a Mass Spectrometer (GC-MS) equipped with RXi -1ms fused silica capillary column. The total phenol content was found to be 112.67+0.03 in ethanolic extract of Tabebuia roseo-alba leaves. The GC-MS analysis revealed the presence of eight major peaks from which various bioactive compounds were ascertained. In conclusion, ethanolic extract of Tabebuia roseo-alba possess many bioactive constituents which may be accountable for its various pharmacological activities.
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Accepted 2021-05-29
Published 2021-09-07
References
Sabithira, G. and Udayakumar, R. GC-MS analysis of methanolic extracts of leaf and stem of Marsilea minuta (Linn.). J. Complemen. Alternat. Med. Res., 2017, 3, 1-13. DOI: https://doi.org/10.9734/JOCAMR/2017/30871
Peter, M.P.J., Yesuraj, J., Sicis, P.P., Joy, V., Saravanan, J. and Sakthivel, S. GC-MS analysis of bioactive components on the leaves extract of Stylosanthes fruticosa- A potential folklore medicinal plant. As. J. Plant Sci. Res., 2012, 2, 243-253. DOI: https://doi.org/10.7439/ijpp.v2i3.418
Nisha, K., Darshana, M., Madhu, G., Bhupendra and M.K. GC-MS analysis and antimicrobial activity of Psidium guajava (leaves) grown in Malva region of India. Int. J. Drug Dev. Res., 2011, 3, 237-245.
Gideon, V.A. GC-MS analysis of phytochemical components of Pseudoglochidion anamalayanum Gamble: An endangered medicinal tree. As. J. Plant Sci. Res., 2015, 5, 36-41.
Arora, S., Kumar, G. and Meena, S. Screening and evaluation of bioactive components of Cenchrus ciliaris L. by GC-MS analysis. Int. Res. J. Pharm., 2017, 8, 69-76. DOI: https://doi.org/10.7897/2230-8407.08699
Lorenzi H. Brazillian Trees. InstitutoPlantarum De Estudos Da Flora, Brazil. 2002.
Silva, J.C., Santos, W.B.D., Araujo, M.G.S., Oliveira, J.D.S., Verissimo, R.C.S.S., Bernardo, T., et al. Evaluation of the cytotoxic, antimicrobial and antioxidant activity of the plant species Tabebuia rosea-alba (ridl) sand. J. Chem. Pharmac. Res., 2017, 9, 148-153.
Lowry, O.H., Rosebrough, N.J., Farr, A. L and Randall, R.J. Protein measurement with Folin phenol reagent. J. Biol. Chem., 1951, 193, 265-275. DOI: https://doi.org/10.1016/S0021-9258(19)52451-6
Sadasivam, S. and Manickam, A. Biochemical methods. 3rd Ed., New age international Limited Publishers, New Delhi. 1996, 8-9.
Singleton, V.L. and Rossi, J.A. Colorimetry of total phenolics with phosphomolbdic phosphor tungstic acid reagents. Am. J. Enol. Vitic., 1965, 16, 144-158.
Ordon, L.E., Gomez, J.D., Vattuone, M.A. and Isla, M.I. Antioxidant activities of Sechium edule (Jacq.) Swart extracts. Fd. Chem., 2006, 97, 452-458. DOI: https://doi.org/10.1016/j.foodchem.2005.05.024
Van Buren, J.P. and Robinson, W.B. Formation of complex between protein and tannic acid. J. Agricul. Fd. Chem., 1969, 17, 772-777 DOI: https://doi.org/10.1021/jf60164a003
Greenwell, M. and Rahman, P.K.S.M. Medicinal plants: Their use in anticancer treatment. Int. J. Pharm. Sci. Res., 2015, 6, 4103-4112.
Sulaiman, C. T. and Balachandran, I. Total phenolics and total flavonoids in selected Indian medicinal plants. Ind. J. Pharm. Sci., 2012, 74, 258-260. DOI: https://doi.org/10.4103/0250-474X.106069
Subedi, L., Timalsena, S., Duwadi, P., Thapa, R., Paudel, A. and Parajuli, K. Antioxidant activity and phenol and flavonoid contents of eight medicinal plants from Western Nepal. J. Tradit. Chin. Med., 2014, 34, 584-590 DOI: https://doi.org/10.1016/S0254-6272(15)30067-4
Pal, D. and Verma, P. Flavonoids: A powerful and abundant source of antioxidants, Int. J. Pharm. Pharma. Sci., 2013, 5, 95-98.
Nazneen Bobby, M.Z., Gnanaraj W.E., Antonysamy, J.M., Adaikalam, A.A.R. and Jamesraj, V. GC-MS Analysis of Albizia lebbeckbenth. World J. Pharm. Res., 2015, 4, 1284-1304.
Swamy, M. K. and Sinniah, U. R. A comprehensive review on the phytochemical constituents and pharmacological activities of Pogostemon cablin benth.: An aromatic medicinal plant of industrial importance. Molec., 2015, 20, 8521-8547. DOI: https://doi.org/10.3390/molecules20058521
Satyal, P., Dosoky, N.S., Poudol, A. and Setzer, W.N. Essential oil constituents and their biologic activities from the leaves of Cassia fistula growing in Nepal. Open Acc. J. Med. Aromat. Plants., 2012, 3, 1-4.
Das, N.M., Sundari, S. S., Karuppausamy, S., Mohan, V.R. and Parthipan, B. GC - MS analysis of leaf and stem bark of Cleidion nitidum (Mull. Arg.) Thwaites Ex Kur, (Euphorbiaceae). As. J. Pharm. Clin. Res., 2014, 7, 41-47.
Ramya, B., Malarvili, T. and Velavan, S. GC-MS Analysis of bioactive compounds in bryonopsis laciniosa fruit extract. Int. J. Pharm. Sci. Res., 2015, 6, 3375-3379.
Sujatha, P.A, Evanjaline, M.R., Muthukumaraswamy, S. and Mohan, V.R. Determination of Bioactive components of Barleria courtallica nees (Acanthaceae) by gas chromatography mass spectrometry analysis. As. J. Pharm. Clin. Res., 2017, 10, 273-283. DOI: https://doi.org/10.22159/ajpcr.2017.v10i6.18035
Casuga, F.P., Castillo, A.L. and Corpuz, M.J.T. GC–MS analysis of bioactive compounds present in different extracts of an endemic plant Broussonetia luzonica (Blanco) (Moraceae) leaves. As. Paci. J. Trop. Biomed., 2016, 6, 957-961. DOI: https://doi.org/10.1016/j.apjtb.2016.08.015
Katiyar, D.D., Singh, V.V., Gilani, S.J., Goel, R., Vats, A. and Bajaj, U. Isolation and characterization of stigmast-5-en-3-ol from heartwood of Berberis aristata. Int. J. Drug Develop. Res., 2014, 6,192-198.