Chemical Composition and in vitro Antioxidant Potential of Tecoma stans (L.) Juss. ex Kunth
DOI:
https://doi.org/10.21048/IJND.2021.58.2.27602Keywords:
Tecoma Stans, Antioxidant, Minerals, Proximate, Phytochemical, Crude Ethanolic Extract And FractionsAbstract
Tecoma stans (L.) Juss. ex Kunth (Bignoniaceae), commonly called yellow-bells, has found wide applications in traditional medicine. In the present study, the nutritional composition, phytochemicals and antioxidant potential of Tecoma stans plant parts was investigated. Carbohydrates ranked first among the macronutrients tailed by protein and ash. Seed displayed significant levels of protein, fat and ash, whereas leaf showed substantial amount of calcium and iron. Polyphenols and flavonoids were predominant in seed and leaf crude ethanolic extracts and fractions as compared to the flower sample. The crude ethanolic extracts and fractions were also evaluated for their scavenging action against DPPH, superoxide anion and hydroxyl radicals. Higher radical scavenging potential was observed in seed and leaf crude extracts and fractions. Among the fractions, n-butanol and ethyl acetate were significantly more efficient than the other fractions against the studied free radicals (p<0.05). These fractions also contained relatively higher levels of total polyphenols and flavonoids than the other fractions of the plant parts. All the crude ethanolic extracts and fractions of the samples showed a significant (p<0.05) dose-dependent radical scavenging action. Strong and positive correlations between phenolic compounds and free radical scavenging activities of Tecoma crude ethanolic extracts and fractions were observed. The obtained results exhibit that the studied plant parts are nutritionally valuable and excellent source of natural bioactive compounds.Downloads
Metrics
Downloads
Published
How to Cite
Issue
Section
License
All the articles published in IJND are distributed under a creative commons license. The journal allows the author(s) to hold the copyright of their work (all usages allowed except for commercial purpose).
Please contact us at editor@informaticsglobal.com for permissions related to commercial use of the article(s).
Accepted 2021-04-28
Published 2021-05-18
References
FAO. In: Some Medicinal Forest Plants of Africa and Latin America, Food and Agriculture Organization of the United Nations, Rome, Italy, 1986, 217-220.
CABI, Global Forestry Compendium (www.cabi.org), 2020.
Pelton, J. A. Survey of the ecology of Tecoma stans. Butler Univer. Bot. Stud., 1964, 14, 53-88.
Winkelman, M. Frequently used medicinal plants in Baja California Norte. J. Ethnopharmacol., 1986, 18, 109-131. https://doi.org/10.1016/0378-8741(86)90024-3. DOI: https://doi.org/10.1016/0378-8741(86)90024-3
Alarcon-Aguilar, F.J. and Roman-Ramos, R. Antidiabetic Plants in Mexico and Central America. In: A. Soumyanath, (Ed.), Traditional medicines for modern times. Antidiabetic plants, CRC Press, Taylor & Francis, Boca Raton, Florida, 2006, 179-194.
Irigoten-Rascon, F. and Paredes, A. In: Raramuri Healers, Tarahumara Medicine: Ethnobotany and healing among the Raramuri of Mexico, University of Oklahoma Press Norman, Oklahoma, Cleveland, 2015. E-PUB: 978-0-0861-5270-7.
Aguilar-Santamaría, L., Ramirez, G., Nicasio, P., Alegria-Reyes, C. and Herrera-Arellano, A. antidiabetic activities of Tecoma stans (L.) Juss. ex Kunth. J. Ethnopharmacol., 2009, 124, 284288.https://doi.org/10.1016/j.jep.2009.04.033. DOI: https://doi.org/10.1016/j.jep.2009.04.033
Kameshwaran, S., Kothai, A.R., Jothimanivannan, C. And Senthilkumar, R. Evaluation of hepatoprotective activity of Tecoma stans flowers. Pharmacologia, 2013, 4, 236-242. DOI: https://doi.org/10.5567/pharmacologia.2013.236.242
Kameshwaran, S., Suresh, V., Arunachalam, G., Kanthlal, S.K. and Mohanraj, M. In vitro and in vivo anticancer activity of methanolic extract of Tecoma stans flowers. Int. Res. J. Pharm., 2012, 3, 246-251.
Taher, M.A., Dawood, D.H., Sanad, M.I. and Hassan, R.A. Searching for anti-hyperglycemicphytomolecules of Tecoma stans. Eur. J. Chem., 2016, 7, 397-404. https://doi.org/10.5155/eurjchem.7.4.397-404.1478 DOI: https://doi.org/10.5155/eurjchem.7.4.397-404.1478
Bakr, R.O., Fayed, M.A.A., Salem, M.A. and Hussein, A.S. Tecoma stans: Alkaloid profile and antimicrobial activity. J. Pharma. Bioall. Sci., 2019, 11, 341-347. https://doi.org/10.4103/ jpbs.JPBS_79_19. DOI: https://doi.org/10.4103/jpbs.JPBS_79_19
Sbihi, H.M., Mokbli, S., Nehdi, I.A. and Al-Resayes, S.I. Physico-chemical properties of Tecoma stans Linn. seed oil: A new crop for vegetable oil. Nat. Prod. Res., 2015, 29, 1249-1255. https:// doi.org/10.1080/14786419. 2015.1024118 DOI: https://doi.org/10.1080/14786419.2015.1024118
Sunamola, A.I., Ngozi, A.O., Vivian, F.D. and Christiana, F.T. Comparative evaluation of the nutritional benefits of some underutilized plant leaves. J. Nat. Prod. Plant Resour., 2012, 2, 261-266.
Abere, T.A. and Enoghama, C.O. Pharmacognostic standardization and insecticidal activity of the leaves of Tecoma stans Juss (Bignoniaceae). J. Sci. Pract. Pharma, 2015, 2, 39-45.
AOAC. In: official methods of analysis, 18th ed. AOAC. International suite, North Fredrick Avenue, Gaithersburg, maryland, USA, 2005.
Sofowora, A. Screening plants for bioactive agents. In: Medicinal plants and traditional medicinal plants in Africa, 2nd Ed, Spectrum books Ltd. Sunshine House, Ibadan, Nigeria, 1993, 134-156.
Trease, G.E. and Evans, W.C. Pharmacognosy, 15th Ed, Saunders Publishers, London, 2002, 221-229.
Singleton, V.L. and Rossi, J.A. Colorimetry of total phenolics with phosphomolybdicphosphotungstic acid reagents. Am. J. Enol. Viticult., 1965, 16, 144-158.
Chang, C., Yang, M., Wen, H. and Chern, J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Fd. Drug Anal., 2002, 10, 178-182. DOI: https://doi.org/10.38212/2224-6614.2748
Brand-Williams, W., Cuvelier, M.E. and Berset, C. Use of a free radical method to evaluate antioxidant activity. LWT- Fd. Sci. Technol., 1995. https://doi.org/10.1016/S0023-6438(95)80008-5 DOI: https://doi.org/10.1016/S0023-6438(95)80008-5
Robak, J. and Gryglewski, R.J. Flavonoids are scavengers of superoxide anions. Biochem. Pharmacol., 1988, 37, 837-841. DOI: https://doi.org/10.1016/0006-2952(88)90169-4
Klein, S.M., Cohen, G. and Cederbaum, A.I. Production of formaldehyde during metabolism of dimethyl sulphoxide by hydroxyl radical generating system. Biochem., 1991, 20, 6006-6012. DOI: https://doi.org/10.1021/bi00524a013
Prajapati, D.K. and Patel, N.M. Pharmacognostic and phytochemical investigations of the leaves of Tecoma stans Linn. Int. J. Pharmaceut. Sci. Rev. Res., 2010, 3, 70-72.
Vargas-Figueroa, J.A. and Torres-Gonzalez, A.M. Germination and seed conservation of a pioneer species, Tecoma stans (Bignoniaceae), from tropical dry forest of Colombia. Revista de Biologia Tropic., 2018, 66, 918-936. DOI: https://doi.org/10.15517/rbt.v66i2.33423
Rao, K.N.V., Swarna, K., Banji, D. and Sandhya, S. Establishment of two varieties in Tecoma stans of Indian origin pharmacognostically and pharmacologically. J. Phytol., 2010, 2, 92-102.
Rodriguez, H.G., Maiti, R., Narvaez, R.I.V. and Sarkar, N.C. Carbon and nitrogen content in leaf tissue of different plant species, north eastern Mexico. Int. J. Bioresour. Stress Manage., 2015, 6, 113-116. https://doi.org/10.5958/0976-4038.2015.00010.X. DOI: https://doi.org/10.5958/0976-4038.2015.00010.X
Agarwal, S.K. and Karthikeyan, V. Quality assessment profile of the leaves of Tecoma stans L. Int. J. Pharmaceut. Res., 2014, 6, 94-99.
Maiti, R., Rodriguez, H.G. and Kumari, A. Nutrient profile of native woody spices and medicinal plants in Northeastern Mexico: A synthesis. J. Bioprocess. Biotech., 2016, 6, 283. https://doi.org/10.4172/2155-9821.1000283. DOI: https://doi.org/10.4172/2155-9821.1000283
Moe, T.M. and Hlaing, A.T. Some useful plants as traditional medicine in Kyaing Tong township. 2nd Myanmar-Korea Conf. Res. J., 2019, 1, 406-413.
Larbie, C., Nyaekoh, C.O. and Adjei, C.O. Phytochemical and safety evaluation of hydroethanolic leaf extract of Tecoma stans (L.) Juss. ex kunth. Evi. based Compl. Alter. Med., 2019, 1-12. DOI: https://doi.org/10.1155/2019/7417624
Thein, M.A. and Oo, K.C.C. Phytochemical investigation of Tecoma stans (L.) H.B.K Leaves and its Brine Shrimp Lethality test. 2nd Myanmar Korea Conf. Res. J., 2019, 1, 1-11.
Farhan, H., Rammal, H., Hijazi, A. and Badran, B. Preliminary phytochemical screening and extraction of polyphenol from stems and leaves of a Lebanese plant Malvaparviflora L. Int. J. Cur. Pharmaceut. Res., 2012, 4, 55-59.
Anand, M., Basavaraju, R. Phytochemical analysis of Tecoma stans (L.) Juss ex kunth leaves from Puttaparthi, Andhra Pradesh, India. Int. J. Cur. Res., 2016, 8, 34632-34635.
Ganga Raju, M., Kasha, G., Mandaloju, S., Suvarchala Reddy, N.V.L. GC-MS and Molecular docking studies of Tecoma stans methanolic leaf extract as potent anti-alzheimer's. Proceedings of International Conference on Drug Discovery. 2020, http://ssrn.com/abstract=3531624.
Govindappa, M., Sadananda, T.S., Channabasava, R., Jeevitha, M.K., Pooja, K.S. and Ragavendra, V.B. Antimicrobial, antioxidant activity and phytochemical screening of Tecoma stans (L.) Juss. ex Kunth. J. Phytol., 2011, 3, 68-76.
Salem, M.Z.M., Gohar, Y.M., Camacho, L.M., El-Shanhorey, N.A. and Salem, A.Z.M. Antioxidant and antibacterial activities of leaves and branches extracts of Tecoma stans (L.) Juss. ex Kunth against nine species of pathogenic bacteria. Afr. J. Microbiol. Res., 2013, 7, 418-426. DOI: https://doi.org/10.5897/AJMR12.2274
Torane, R.C. Kamble, G.S. Adsul, V.B. Shendkar, C.D. and Deshpande, N.R. Antioxidant activity of aerial parts of Tecoma stans. Int. J. Chem. Anal. Sci., 2011, 2, 130-132.
Prathibha, R. Niranjan Kumar, A. Srinivas, K. V. N. S. Kotesh Kumar, J. Srivani, A. and Krishna Mohan, G. In vitro evaluation of antioxidant activity, estimation of total phenolic and flavonoid content of different extracts of Tecoma stans (L.). Int. J. Pharm Biol. Sci., 2018, 8, 962-968.
Ramirez, G., Zamilpa, A., Zavala, M., Perez, J., Morales, D. And Jaime, T. Chrysoeriol and other polyphenols from Tecoma stans with lipase inhibitory activity. J. Ethnopharmacol., 2016, 185, 1-8. DOI: https://doi.org/10.1016/j.jep.2016.03.014
Constantino, L., Raimondi, L., Pirisino, R., Brunetti, T., Pessotto, P., Giannessi, F., Lins, A.P., Barlocco, D., Antolini, L. and El-Abady, S.A. Isolation and pharmacological activities of the Tecoma stans alkaloids. II Farmaco, 2003, 58, 781-785. DOI: https://doi.org/10.1016/S0014-827X(03)00133-2
Marzouk, M., Gamal-Eldeen, A., Mohamed, M. and El-Sayed, M. Anti-proliferative, antioxidant constituents from Tecoma stans. Z. Naturforsch, 2006, 61,783-791.
Ranjit, P.M., Nagarani, T., Swathi, V., Pahni Kumar, K., Chowdary, Y.A., Siva Reddy, C.H. and Girijasankar, G. Evaluation of phytochemical content and in vitro cytotoxic activity of various ornamental plant flower extracts against MCF-7 cell lines. Int. J. Cur. Res. Life Sci., 2015, 4, 172-176.
Taha, M.M. The carotenoids of the petals of two species of Tecoma. Biochem. J., 1954, 58, 413-415. DOI: https://doi.org/10.1042/bj0580413
Zheng, C.D., Li, G., Li, H.Q., Xu, X.J., Gao, J.M. and Zhang, A.L. DPPH-scavenging activities and structure-activity relationships of phenolic acids. Nat. Prod. Commun., 2010, 5, 1759-1765. DOI: https://doi.org/10.1177/1934578X1000501112
Singh, R.P., Murthy, K.N.C. and Jayaprakasha, G.K. Studies on antioxidant activity of pomegranate (Punicagranatum) peel and seed extracts using in vitro models. J. Agric. Fd. Chem., 2002, 50, 81-86. DOI: https://doi.org/10.1021/jf010865b
Korycka-Dahl, M. and Richardson, T. Photogeneration of superoxide anion in serum of bovine milk and in model systems containing riboflavin and amino acids. J. Dairy Sci., 1978, 61, 400407. DOI: https://doi.org/10.3168/jds.S0022-0302(78)83613-3