In vitro Evaluation of Crude Extracts and Isolated Compounds from Goniothalamus rongklanus and Goniothalamus latestigma for Bioactive Properties

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Authors

  • Nutthapol Funnimid
     Faculty of Science, Laboratory of Natural Products, Center of Excellence for Innovation in Chemistry, Lampang Rajabhat University, 52100 Lampang ,TH
  • Wilart Pompimon
     Faculty of Science, Laboratory of Natural Products, Center of Excellence for Innovation in Chemistry, Lampang Rajabhat University, 52100 Lampang ,TH
  • Narong Nuntasaen
     The Forest Herbarium, Department of National Park, Wildlife and Plant Conservation, Ministry of Natural Resources and Environment, 10900 Bangkok ,TH

DOI:

https://doi.org/10.18311/jnr/2019/23766

Keywords:

Antibacterial Activity, Anti-HIV-1 RT, Cytotoxicity, Goniothalamus rongklanus, Goniothalamus lagestima
Natural Product Chemistry

Abstract

The genus Goniothalamus belongs to the Annonaceae family. The pure compounds from Goniothalamus rongklanus and Goniothalamus latestigma were isolated by repeated column chromatography. The structures were recognized by NMR spectral methods. The chemical compounds of ethyl acetate extract (EtOAc) from the stems of G. rongklanus were identified as goniotriol (1) and stigmasterol glucoside (2). The EtOAc leaves extract of G. latestigma yielded a compound, which was identified as pinocembrin (3). The ethyl acetate (EtOAc) and methanol (MeOH) extract were tested for anti-HIV-1 RT and cytotoxic activities against P-388, KB, HT 29, MCF-7, A 549, ASK and CL cell lines. The EtOAc and MeOH extract of G. rongklanus showed evidence of anti-HIV-1 RT inhibition at 76.44 and 88.48 %, respectively. The EtOAc extract of G. rongklanus and G. latestigma showed cytotoxic activities on KB and HT 29 with an ED50 at <4 μg/mL. In addition, antibacterial study on extracts and isolated compounds was also performed. Antibacterial study was evaluated using nine strains (Staphylococcus aureus, Enterobacter aerogenes, Escherichia coli 0157: H7, Escherichia coli (ETEC), Escherichia coli (EPEC), Proteus mirabilis, Salmonella typhimuriam, Shigella flexneri and Vibrio cholera) by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) method. Goniotriol was the most effective antibacterial with a MIC in the range <0.16 - 0.6 mg/mL and MBC in the range 0.3 - >5 mg/mL. The antibacterial activity of goniotriol has been reported for the first time.

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Published

2019-09-18

How to Cite

Funnimid, N., Pompimon, W., & Nuntasaen, N. (2019). <i>In vitro</i> Evaluation of Crude Extracts and Isolated Compounds from <i>Goniothalamus rongklanus</i> and <i>Goniothalamus latestigma</i> for Bioactive Properties. Journal of Natural Remedies, 19(3), 146–152. https://doi.org/10.18311/jnr/2019/23766

Issue

Volume 19, Issue 3, July 2019

Section

Research Articles
Crossref
Scopus
Google Scholar
Europe PMC
Received 2019-05-29
Accepted 2019-08-14
Published 2019-09-18

 

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