Bioactivity Screening and Identification of Secondary Metabolites from Fungal Endophytes of Carica papaya L. Leaves

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Authors

  • Israt Farha Lini
     Pharmaceutical Sciences Research Division, BCSIR Laboratories, Dhaka, Dr. Qudrat-I-Khuda Road, Dhanmondi, Dhaka – 1205 ,BD
  • Rabita Zinnurine
     Pharmaceutical Sciences Research Division, BCSIR Laboratories, Dhaka, Dr. Qudrat-I-Khuda Road, Dhanmondi, Dhaka – 1205 ,BD
  • Md. Habibur Rahman
     Dr. Mahbubur Rahman Mollah College, Matuail, Jatrabari, Dhaka – 1362 ,BD
  • Mst. Nadira Begum
     Biological Research Division, BCSIR Laboratories, Dhaka, Dr. Qudrat-I-Khuda Road, Dhanmondi, Dhaka – 1205 ,BD
  • Farhana Afroz
     Pharmaceutical Sciences Research Division, BCSIR Laboratories, Dhaka, Dr. Qudrat-I-Khudan Road, Dhanmondi, Dhaka – 1205 ,BD
  • Satyajit Roy Rony
     Pharmaceutical Sciences Research Division, BCSIR Laboratories, Dhaka, Dr. Qudrat-I-Khudan Road, Dhanmondi, Dhaka – 1205 ,BD
  • Suriya Sharmin
     Pharmaceutical Sciences Research Division, BCSIR Laboratories, Dhaka, Dr. Qudrat-I-Khudan Road, Dhanmondi, Dhaka – 1205 ,BD
  • Choudhury Mahmood Hasan
     Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka – 1000 ,BD
  • Md. Hossain Sohrab
     Pharmaceutical Sciences Research Division, BCSIR Laboratories, Dhaka, Dr. Qudrat-I-Khuda Road, Dhanmondi, Dhaka – 1205 ,BD

DOI:

https://doi.org/10.18311/jnr/2020/24110

Keywords:

Colletotrichum, Carvularia, Alternaria, Ergosterol, Alternariol Monomethyl Ether
Natural Chemistry

Abstract

Objectives: This work describes the chemical and in-vitro bioactivity studies on endophytic fungi derived from the leaves of Carica papaya L. Methods: Column Chromatography was employed for isolation of fungal metabolites. Nuclear Magnetic Resonance (NMR) spectral data was utilized to elucidate the structure of the isolated compounds and physicochemical properties of them were also examined. As a part of in-vitro bioactivity screening disc diffusion method, 1, 1-diphenyl-2- picryl-hydrazyl (DPPH) scavenging assay and brine shrimp lethality bioassay were conducted to evaluate antimicrobial, antioxidant and cytotoxic activities, respectively. Results: A total of four fungal isolates were identified as endophytic fungi and purified from the young leaves of Carica papaya. These fungi, encoded as CPLE-1, CPLE-2, CPLE-3 and CPLE-4, were identified up to the genus level on the basis of their macroscopic and microscopic characteristics as Colletotrichum sp., Carvularia sp., and next two as Alternaria sp., respectively. Chemical profiling and preliminary bioactivity screening of the fungal endophytes signified strain CPLE-3 with highest potentiality to produce bioactive compounds which directs its large scale cultivation. Ergosterol and Alternariol monomethyl ether were isolated from the ethyl acetate extract of the fungus, CPLE-3. Conclusion: Bioactivity and chemical screening of the endophytic fungal extracts of Carica papaya leaf along with the isolation of fungal secondary metabolites suggested these endophytic fungi to be possible source of bioactive leads for developing new and improved medicines.

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Published

2021-01-13

How to Cite

Farha Lini, I., Zinnurine, R., Habibur Rahman, M., Nadira Begum, M., Afroz, F., Roy Rony, S., Sharmin, S., Mahmood Hasan, C., & Hossain Sohrab, M. (2021). Bioactivity Screening and Identification of Secondary Metabolites from Fungal Endophytes of <i>Carica papaya</i> L. Leaves. Journal of Natural Remedies, 20(4), 217–227. https://doi.org/10.18311/jnr/2020/24110

Issue

Volume 20, Issue 4, October 2020

Section

Research Articles
Crossref
Scopus
Google Scholar
Europe PMC
Received 2019-08-21
Accepted 2020-08-25
Published 2021-01-13

 

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