DNA Barcoding Markers and Next Generation Sequence Analysis of Rbcl in Intraspecies Genetic Variations of Three Varieties of Cissus quadrangularis L.

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Authors

  • Goli Penchala Prasad
     Regional Ayurveda Research Institute for Fundamental Research, Kothrud, Pune – 411038, Maharashtra ,IN
  • G. P. Pratap
     Central Research Institute of Unani Medicine, Hyderabad – 500038, Telangana ,IN
  • Srinivasan Marimuthu
     Regional Ayurveda Research Institute for Fundamental Research, Kothrud, Pune – 411038, Maharashtra ,IN
  • Shyam Baboo Prasad
     Regional Ayurveda Research Institute for Fundamental Research, Kothrud, Pune – 411038, Maharashtra ,IN
  • Arun Manohar Gurav
     Regional Ayurveda Research Institute for Fundamental Research, Kothrud, Pune – 411038, Maharashtra ,IN
  • Anupam Kumar Mangal
     Central Council for Research in Ayurvedic Sciences, Janakpuri – 110058, New Delhi ,IN
  • N. Srikanth
     Central Council for Research in Ayurvedic Sciences, Janakpuri – 110058, New Delhi ,IN

DOI:

https://doi.org/10.18311/jnr/2020/24387

Keywords:

Ayurveda, Cissus quadrangularis L., Mat k, Medicinal Plants, Next-Generation Sequencing, Rbcl, ITS4

Abstract

Cissus quadrangularis L. is a well-known plant used in Ayurveda. It is also called Asthisamharaka. The etymology of its name indicates its use in the healing of the fractured bone. It is a widely growing plant belonging to the family of vitaceae. C. quadrangularis (Family: Vitaceae) is commonly distributed throughout the hotter parts of India and Sri Lanka. C. quadrangularis is mentioned in classical literature like Bhava Prakash and Chakra Dutta. The present study design to compare three different varieties of C. quadrangularis which includes the species of quadrangular, round and flat types are used to study DNA barcoding marker and phylogenetic analysis. Whole-genome was isolated from the stem parts of three varieties of C. quadrangularis; quadrangular, round and flat. The PCR amplification of DNA barcoding markers (rbcl, mat k and ITS) was analyzed by DNA electrophoresis. The DNA barcoding markers and next-generation sequencing are able to identify the intraspecies genetic variations among these closely related plant varieties of C. quadrangularis of quadrangular, round and flat.

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Published

2020-06-11

How to Cite

Penchala Prasad, G., Pratap, G. P., Marimuthu, S., Baboo Prasad, S., Manohar Gurav, A., Kumar Mangal, A., & Srikanth, N. (2020). DNA Barcoding Markers and Next Generation Sequence Analysis of Rbcl in Intraspecies Genetic Variations of Three Varieties of <i>Cissus quadrangularis</i> L. Journal of Natural Remedies, 20(2), 103–109. https://doi.org/10.18311/jnr/2020/24387

Issue

Volume 20, Issue 2, April 2020

Section

Research Articles
Crossref
Scopus
Google Scholar
Europe PMC
Received 2019-10-30
Accepted 2020-02-19
Published 2020-06-11

 

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