Identification and Quantification by HPLCDAD of Furosemide as a Co-adulterant in Products of Natural Origin

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Authors

  • Flávia Bonfim Lima
     Laboratório de Análises Clí­nicas, Centro Universitário Saúde ABC/ Faculdade de Medicina do ABC, Av, Lauro Gomes, 2000, 09060-870, Santo André, SP ,BR
  • Beatriz da Costa Aguiar Alves
     Laboratório de Análises Clí­nicas, Centro Universitário Saúde ABC/ Faculdade de Medicina do ABC, Av, Lauro Gomes, 2000, 09060-870, Santo André, SP ,BR
  • David Feder
     Laboratório de Análises Clí­nicas, Centro Universitário Saúde ABC/ Faculdade de Medicina do ABC, Av, Lauro Gomes, 2000, 09060-870, Santo André, SP ,BR
  • Marina Cristina Peres
     Laboratório de Análises Clí­nicas, Centro Universitário Saúde ABC/ Faculdade de Medicina do ABC, Av, Lauro Gomes, 2000, 09060-870, Santo André, SP ,BR
  • Glaucia Luciano da Veiga
     Laboratório de Análises Clí­nicas, Centro Universitário Saúde ABC/ Faculdade de Medicina do ABC, Av, Lauro Gomes, 2000, 09060-870, Santo André, SP ,BR
  • Edimar Cristiano Pereira
     Departamento de Ciíªncias Farmacíªuticas da Universidade Federal de Sào Paulo, Rua Prof. Artur Riedel, 275, 09972-270, Diadema, SP ,BR
  • Fernando Luiz Affonso Fonseca
     Laboratório de Análises Clí­nicas, Centro Universitário Saúde ABC/ Faculdade de Medicina do ABC, Av, Lauro Gomes, 2000, 09060-870, Santo André, SP ,BR

DOI:

https://doi.org/10.18311/jnr/2020/24396

Keywords:

Chromatography, Co-Adulterant, Furosemide, HPLC, Natural Products

Abstract

The low cost added to easy access and expectation of low or no side effects make these products increasingly attractive. When a product of natural origin contains synthetic substances that are not declared in its formulation, the synthetic substance is characterized as adulteration. In order to identify and quantify adulterants in natural products, analytical methods have been developed and used as fundamental tools in the control of these products. Thus, two products of natural origin indicated for treatment of rheumatic and inflammatory diseases were analyzed to verify the presence of the co-adulterant furosemide. Co-adulterant presence in the products was tested using an Agilent® brand 1100 HPLC system with a quaternary pump, an automatic injector and a DAD detector, with a mobile phase composed of methanol/formic acid 0.2% 60/40 (v/v). HPLC-DAD indicates the presence of the undeclared furosemide compound in the original formulation of both analyzed samples. In sample A, 24 mg of furosemide per gram was found, while in sample B, 47mg per gram of product was obtained. The consumption of adulterated products may lead to risks such as drug interaction and intoxication, since active ingredients of synthetic origin are added without taking in consideration adjustments and quality of the raw material.

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Published

2020-10-01

How to Cite

Bonfim Lima, F., da Costa Aguiar Alves, B., Feder, D., Cristina Peres, M., Luciano da Veiga, G., Cristiano Pereira, E., & Luiz Affonso Fonseca, F. (2020). Identification and Quantification by HPLCDAD of Furosemide as a Co-adulterant in Products of Natural Origin. Journal of Natural Remedies, 20(3), 166–172. https://doi.org/10.18311/jnr/2020/24396

Issue

Volume 20, Issue 3, July 2020

Section

Research Articles
Crossref
Scopus
Google Scholar
Europe PMC
Received 2019-11-01
Accepted 2020-05-01
Published 2020-10-01

 

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