Relaxant and Antispasmodic Activities of Aqueous Extract from Thymus algeriensis Boiss. and Reut.

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Authors

  • Leila Beyi
     Laboratory of Bioressources, Biotechnologies, Ethnopharmacologie et Santé, Faculty of Sciences, Mohamed the First University, PB 717, 60000, Oujda ,MA ORCID logo http://orcid.org/0000-0002-3006-2523
  • Hassan Zrouri
     Laboratory of Bioressources, Biotechnologies, Ethnopharmacologie et Santé, Faculty of Sciences, Mohamed the First University, PB 717, 60000, Oujda ,MA
  • Hanane Makrane
     Laboratory of Bioressources, Biotechnologies, Ethnopharmacologie et Santé, Faculty of Sciences, Mohamed the First University, PB 717, 60000, Oujda ,MA
  • Hassane Mekhfi
     Laboratory of Bioressources, Biotechnologies, Ethnopharmacologie et Santé, Faculty of Sciences, Mohamed the First University, PB 717, 60000, Oujda ,MA
  • Abderrahim Ziyyat
     Laboratory of Bioressources, Biotechnologies, Ethnopharmacologie et Santé, Faculty of Sciences, Mohamed the First University, PB 717, 60000, Oujda ,MA
  • Mohamed Bnouham
     Laboratory of Bioressources, Biotechnologies, Ethnopharmacologie et Santé, Faculty of Sciences, Mohamed the First University, PB 717, 60000, Oujda ,MA
  • Mohammed Aziz
     Laboratory of Bioressources, Biotechnologies, Ethnopharmacologie et Santé, Faculty of Sciences, Mohamed the First University, PB 717, 60000, Oujda ,MA

DOI:

https://doi.org/10.18311/jnr/2021/24752

Keywords:

Antispasmodic, Jejunum, Relaxant, Smooth muscle, Thymus algeriensis

Abstract

Thymus algeriensis have been vastly utilized for intestinal disorders. The purpose of this investigation was to scrutinize the probable mechanism for its utilization in the spasm disorder. Aqueous extract of this medicinal plant (AqTA) was tested in vitro on rat and rabbit jejunum. The extract produced relaxation of rabbit jejunum. This relaxation does not depend on the adrenergic pathway, the AqTA induces inhibition irrespective of the presence or absence of adrenergic inhibitors. AqTA engendered a concentration-dependent (0.1-5 mg/ml) relaxation of carbamylcholine chloride (CCh) and K+ provoked tones in rat intestine with IC50 values of 2.06 ± 0.26 and 3.55 ± 0.48 (mg/ml) respectively. This extract likewise induced a dosedependent (0.1-3 mg/ml) rightward shift in the CCh and Ca++ dose-response curves. The AqTA alone has decreased more significantly the percentage of contraction of rat jejunum than the AqTA pre-incubated with atropine or hexamethonium then contracted with KCl; but there is no significant difference by those pre-incubated with methylene blue or L-NAME. When the intestine was pretreated with nifedipine and contracted by CCh, the antispasmodic effect provoked by AqTA with and without pre-incubation with nifedipine is statistically not significant. In conclusion AqTA acts possibly on the voltage dependent Ca++ channel and cholinergic receptors but did not act on adrenergic receptors, NO and guanylatecyclase pathway. This investigation may explicate some of its traditional utilization in gut illnesses.

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Published

2021-04-17

How to Cite

Beyi, L., Zrouri, H., Makrane, H., Mekhfi, H., Ziyyat, A., Bnouham, M., & Aziz, M. (2021). Relaxant and Antispasmodic Activities of Aqueous Extract from <i>Thymus algeriensis</i> Boiss. and Reut. Journal of Natural Remedies, 21(2), 165–171. https://doi.org/10.18311/jnr/2021/24752

Issue

Volume 21, Issue 2, April 2021

Section

Short Communication
Crossref
Scopus
Google Scholar
Europe PMC
Received 2020-01-08
Accepted 2020-12-15
Published 2021-04-17

 

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