In Vivo Phytotherapy in BALB/c Athymic Nude Mice: Hair Growth Promotion using Ficus religiosa L. and Morus alba L.

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Authors

  • Pramod S. Joshi
     Symbiosis School of Biological Sciences, Symbiosis International (Deemed University), Pune, Maharashtra – 412115 ,IN
  • Yogesh B. Patil
     Symbiosis Centre for Research and Innovation, Symbiosis International (Deemed University), Pune, Maharashtra – 412115 ,IN
  • Bhagyashri Nagarkar
     APT Research Foundation, Pune, Maharashtra – 411041 ,IN
  • Tania S. Paul
     APT Research Foundation, Pune, Maharashtra – 411041 ,IN
  • Kishori G. Apte
     APT Research Foundation, Pune, Maharashtra – 411041 ,IN

DOI:

https://doi.org/10.18311/jnr/2021/26255

Keywords:

Anagen Phase, Ficus religiosa L., Hair Follicles, Morus alba L., Nude Mouse

Abstract

Ficus religiosa L. (FR) and Morus alba L. (ML) belonging to the family Moraceae have been tested as novel herbal agents for hair growth promotion and Hair Follicles (HFs) regeneration in BALB/c athymic nude mouse model. Current study tested different mixtures of 5% aqueous fractions: Test 1 (ML2+ML3+ML4+FR4), Test 2 (FR1+FR2+FR4), or Test 3 (ML2+ML3+ML4+FR1+FR2+FR4) from leaves of both plants including standard of care 2% minoxidil. Control mice were untreated. Animals were treated for 33 days by topical application on the back skin and changes in hair growth patterns were evaluated. Histology was performed to assess the HFs morphology, and modulation of hair cycle phases. Gene expression analysis was performed to understand potential mechanisms of action. All treatment groups had significantly higher anagen phase HFs compared with untreated control group based on histology analysis. Also, expressions of CD34, CD200R and Oct4 genes were upregulated in all treatment groups compared with untreated control. Present study demonstrated that a combinatorial therapy using either fractions of FR or FR and ML promoted hair growth and HFs regeneration through induction of anagen phase in conjunction with stem cells associated genes upregulation in BALB/c athymic nude mouse model of hair loss.

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Published

2021-02-26

How to Cite

Joshi, P. S., Patil, Y. B., Nagarkar, B., Paul, T. S., & Apte, K. G. (2021). In Vivo Phytotherapy in BALB/c Athymic Nude Mice: Hair Growth Promotion using <i>Ficus religiosa</i> L. and <i>Morus alba</i> L. Journal of Natural Remedies, 21(1), 51–60. https://doi.org/10.18311/jnr/2021/26255

Issue

Volume 21, Issue 1, January 2021

Section

Research Articles
Crossref
Scopus
Google Scholar
Europe PMC
Received 2020-10-14
Accepted 2020-12-21
Published 2021-02-26

 

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