Evaluation and Assessment of the Acute Toxic Potential of Sansevieria cylindrica and Plumeria obtusa Plant Extracts in Wistar Albino Rats

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Authors

  • Sunil Shewale
     Department of Pharmacology, Dr. D. Y. Patil Institute of Pharmaceutical Sciences & Research, Savitribai Phule Pune University, Maharashtra - 411018 ,IN
  • Vaishali Undale
     Department of Pharmacology, Dr. D. Y. Patil Institute of Pharmaceutical Sciences & Research, Savitribai Phule Pune University, Maharashtra - 411018 ,IN
  • Vrushali Bhalchim
     Department of Pharmacology, Dr. D. Y. Patil Institute of Pharmaceutical Sciences & Research, Savitribai Phule Pune University, Maharashtra - 411018 ,IN
  • Shivani Desai
     Department of Pharmacology, Dr. D. Y. Patil Institute of Pharmaceutical Sciences & Research, Savitribai Phule Pune University, Maharashtra - 411018 ,IN
  • Maruti Shelar
     Department of Pharmacognosy, Dr. D. Y. Patil Institute of Pharmaceutical Sciences & Research, Savitribai Phule Pune University, Maharashtra - 411018 ,IN
  • Shubham Padole
     Department of Pharmacology, Dr. D. Y. Patil Institute of Pharmaceutical Sciences & Research, Savitribai Phule Pune University, Maharashtra - 411018 ,IN
  • Sohan Chitlange
     Department of Pharmaceutical Chemistry, Dr. D. Y. Patil Institute of Pharmaceutical Sciences & Research, Savitribai Phule Pune University, Maharashtra - 411018 ,IN
  • Vikas Wawale
     Department of Quality Assurance, Synapse Labs Pvt. Ltd., Pune, Maharashtra - 411014 ,IN
  • Sameer Parekh
     Department of Clinical Research & Pharmacovigilance, Serum Institute of India Pvt. Ltd., Pune, Maharashtra - 411028 ,IN
  • Pramod Pujari
     Department of Clinical Research & Pharmacovigilance, Serum Institute of India Pvt. Ltd., Pune, Maharashtra - 411028 ,IN

DOI:

https://doi.org/10.18311/jnr/2022/28768

Keywords:

Acute Toxicity, Plumeria obtusa, Safety Assessment, Sansevieria cylindrica
Pharmacology

Abstract

Sansevieria cylindrica (SC) Bojer ex Hook. (Asparagaceae) and Plumeria obtusa (PO) L. (Apocynaceae) are indoor and outdoor ornamental plants respectively. These plants are traditionally used by the local healers during accidental injuries. However, their toxicological properties are very poorly explored over folkloric usage. Therefore, the present study evaluated the toxic potencies of SC leaves and PO seed Hydro-Alcoholic Extract (SCPOHAE) through acute oral dose (14-days) administration in female Wistar rats. Safety of the SCPOHAE was evaluated as per Organization for Economic Co-operation and Development (OECD) Acute Oral Toxicity study guidelines 423. The female Wistar rats were divided into three groups (n=3). A single oral dose of 2000 mg/kg of body weight of individual extract and 1:1 blend was administered to each animal. The animals were closely observed for clinical signs, neurobehavioral changes, morbidity, and mortality if any for the first half an hour and then every hour for the first four hours followed by observation every 24–hours for 14 days. Changes in food and water consumption, body weight were monitored daily during the study. On day 1 and day 15 blood samples were collected to evaluate changes in the hematology and biochemistry parameters. The urine samples were also collected for urine analysis parameters. Animals were sacrificed on day 15 and organ samples of liver and kidney were collected for histopathological findings. The SCPOHAE individually and also as 1:1 blend at the limit dose (2000 mg/kg, body weight) did not cause death and did not induce any remarkable and abnormal clinical signs, indicative of systemic toxicity, in rats during the treatment period of 14–days. The statistically non-significant small differences in the body weight were observed. Conclusion: The oral administration of SCPOHAE did not cause any systemic toxic effects. In conclusion, the No-observed-Adverse-Effect Level (NOAEL) of these extracts in rats was found to be greater than 2000 mg/kg.

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Published

2022-04-19

How to Cite

Shewale, S., Undale, V., Bhalchim, V., Desai, S., Shelar, M., Padole, S., Chitlange, S., Wawale, V., Parekh, S., & Pujari, P. (2022). Evaluation and Assessment of the Acute Toxic Potential of <i>Sansevieria cylindrica</i> and </i>Plumeria obtusa</i> Plant Extracts in Wistar Albino Rats. Journal of Natural Remedies, 22(2), 209–220. https://doi.org/10.18311/jnr/2022/28768

Issue

Volume 22, Issue 2, April 2022

Section

Research Articles
Crossref
Scopus
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Europe PMC
Received 2021-10-06
Accepted 2022-02-18
Published 2022-04-19

 

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