Phytochemical Profiling of Various Extracts of Glycine max (L.) Seeds and In-silico Approach for Hepato-Protective Activity

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Authors

  • S. Vishnupriya
     Department of Food Science and Nutrition, Avinashilingam Institute for Home Science and Higher Education for Women, Deemed University, Coimbatore - 641043, Tamil Nadu ,IN
  • S. Kowsalya
     Department of Food Science and Nutrition, Avinashilingam Institute for Home Science and Higher Education for Women, Deemed University, Coimbatore - 641043, Tamil Nadu ,IN

DOI:

https://doi.org/10.18311/jnr/2022/29021

Keywords:

Choline, Glycine max (L.), NF-KB, PPARα, PXR, TGF-β

Abstract

Cirrhosis and fibrosis are mainly characterized by the frequent and repeated inflammation of the renal cells. These renal disorders may also lead to hepatocarcinoma and even death, so we are in need of complementary and alternative medicine to treat renal diseases. The Soybean (Glycine max (L.) Merr.) seed is reported to have medicinal properties and pharmacological activities like anti-inflammatory and anti-oxidant. Here, we have evaluated various extracts (Water, Ethanol, Methanol, Hexane and Benzene) of Glycine max (L.) to predict their phytoconstituents and found that methanolic extract has more phytoconstituents. The finest chemicals in the methanolic extract, such as 3-Methoxy-hexane-1,6- diol, Choline, 9,12,15-Octadecatrien-1-ol, and tetradecane, were docked against Transforming Growth Factor-(TGF-β) a cytokine, transcription factors such as Peroxisome Proliferator-Activated Receptor (PPAR) and Pregnene X Receptor (PXR), and Nuclear Factor kappa-b (NF-KB) a protein complex. Comparatively, Choline shows higher negative binding energies against all the receptors and possesses hepato-protective activity.

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Published

2022-12-16

How to Cite

Vishnupriya, S., & Kowsalya, S. (2022). Phytochemical Profiling of Various Extracts of <i>Glycine max</i> (L.) Seeds and <i>In-silico</i> Approach for Hepato-Protective Activity. Journal of Natural Remedies, 22(4), 607–616. https://doi.org/10.18311/jnr/2022/29021

Issue

Volume 22, Issue 4, October 2022

Section

Research Articles

License

Copyright (c) 2022 Vishnupriya S, Kowsalya S

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Europe PMC
Received 2021-11-23
Accepted 2022-03-22
Published 2022-12-16

 

References

Tanwar S, Rhodes F, Srivastava A, Trembling PM, Rosenberg WM. Inflammation and fibrosis in chronic liver diseases including non-alcoholic fatty liver disease and hepatitis C. World J Gastroenterol. 2020; 26(2):109-33. https://doi. org/10.3748/wjg.v26.i2.109 DOI: https://doi.org/10.3748/wjg.v26.i2.109

Rendic SP, Peter Guengerich F. Human cytochrome P450 enzymes 5-51 as targets of drugs and natural and environmental compounds: mechanisms, induction, and inhibition - toxic effects and benefits. Drug Metab Rev. 2018; 50(3):256- 342. https://doi.org/10.1080/03602532.2018.1483401 DOI: https://doi.org/10.1080/03602532.2018.1483401

Musso G, Gambino R, Cassader M. Non-alcoholic fatty liver disease from pathogenesis to management: an update. Obes Rev. 2010; 11(6):430-45. https://doi.org/10.1111/j.1467-789X.2009.00657.x DOI: https://doi.org/10.1111/j.1467-789X.2009.00657.x

Orman ES, Roberts A, Ghabril M, Nephew L, Desai AP, Patidar K, Chalasani N. Trends in Characteristics, Mortality, and Other Outcomes of Patients With Newly Diagnosed Cirrhosis. JAMA Netw Open. 2019; 2(6):e196412. https://doi.org/10.1001/jamanetworkopen.2019.6412 DOI: https://doi.org/10.1001/jamanetworkopen.2019.6412

Schutte K, Schulz C, Poranzke J, Antweiler K, Bornschein J, Bretschneider T, Arend J, Ricke J, Malfertheiner P. Characterization and prognosis of patients with hepatocellular carcinoma (HCC) in the non-cirrhotic liver. BMC Gastroenterol. 2014; 14:117. https://doi.org/10.1186/1471- 230X-14-117 DOI: https://doi.org/10.1186/1471-230X-14-117

Wiegand J, Berg T. The etiology, diagnosis and prevention of liver cirrhosis: part 1 of a series on liver cirrhosis. Dtsch Arztebl Int. 2013; 110(6):85-91. https://doi.org/10.3238/arztebl.2013.0085 DOI: https://doi.org/10.3238/arztebl.2013.0085

Purushothaman B, PrasannaSrinivasan R, Suganthi P, Ranganathan B, Gimbun J, and Shanmugam K. A Comprehensive Review on Ocimum basilicum. Journal of Natural Remedies. 2018; 18(3):71-85. https://doi.org/10.18311/jnr/2018/21324 DOI: https://doi.org/10.18311/jnr/2018/21324

Saranya, Kalimuthu J, Balakrishnan P, Ramalingam PS, Parthasarathi S, Ganesan B, Kaliyaperumal R, Ranganathan B and Shanmugam S. Isolation and characterisation of cellulolytic activity of bacteria and fungi from the soil of paper recycling unit at periyar maniammai university. Indo Ame J. Pharma Res. 2017; 7(6):8253-64.

Ramalingam PS, Sagayaraj M, Ravichandiran P, Balakrishnanan P, Nagarasan S, Shanmugam K. Lipid peroxidation and anti-obesity activity of Nigella sativa seeds. W. J. Pharm. Res. 2017; 6(10):882-92. https://doi.org/10.20959/wjpr201710-9389 DOI: https://doi.org/10.20959/wjpr201710-9389

Balakrishnan P, Kumar GS, Ramalingam PS, Nagarasan S, Murugasan V, Shanmugam K. Distinctive pharmacological activities of Eclipta alba and it’s coumestan wedolactone. Indo Ame. J. Pharm. Res. 2018; 5(4):2996-3002.

Rizzo G, Baroni L. Soy, Soy Foods and Their Role in Vegetarian Diets. Nutrients. 2018 Jan 5; 10(1):43. https:// doi.org/10.3390/nu10010043 DOI: https://doi.org/10.3390/nu10010043

Messina M. Soy and Health Update: Evaluation of the Clinical and Epidemiologic Literature. Nutrients. 2016; 8(12):754. https://doi.org/10.3390/nu8120754 DOI: https://doi.org/10.3390/nu8120754

Wang Y, Liu L, Ji F, Jiang J, Yu Y, Sheng S, Li H. Soybean (Glycine max) prevents the progression of breast cancer cells by down-regulating the level of histone demethylase JMJD5. J Cancer Res Ther. 2018; 14(Supplement):S609-S615. https://doi.org/10.4103/0973-1482.187292 DOI: https://doi.org/10.4103/0973-1482.187292

Ghahari S, Alinezhad H, Nematzadeh GA, Tajbakhsh M, Baharfar R. Chemical Composition, Antioxidant and Biological Activities of the Essential Oil and Extract of the Seeds of Glycine max (Soybean) from North Iran. Curr Microbiol. 2017; 74(4):522-531. https://doi.org/10.1007/ s00284-016-1188-4 DOI: https://doi.org/10.1007/s00284-016-1188-4

Durango D, Murillo J, Echeverri F, Escobar G, Quinones W. Isoflavonoid composition and biological activity of extracts from soybean seedlings treated by different elicitors. An Acad Bras Cienc. 2018; 90(2 suppl 1):1955-71. https://doi.org/10.1590/0001-3765201820170785 DOI: https://doi.org/10.1590/0001-3765201820170785

Naim M, Gestetner B, Bondi A, Birk Y. Antioxidative and antihemolytic activities of soybean isoflavones. J Agric Food Chem. 1976; 24(6):1174-7. https://doi.org/10.1021/jf60208a029 DOI: https://doi.org/10.1021/jf60208a029

Lambert C, Bellemere G, Boyer G, Ponelle F, Bauer T, Legeny MC, Baudouin C, Henrotin Y. Composition Analysis and Pharmacological Activity of Avocado/ Soybean Unsaponifiable Products Used in the Treatment of Osteoarthritis. Front Pharmacol. 2022; 12:781389. https://doi.org/10.3389/fphar.2021.781389 DOI: https://doi.org/10.3389/fphar.2021.781389

Mekala JR, Kurappalli RK, Ramalingam P, Moparthi NR. N-acetyl l-aspartate and Triacetin modulate tumor suppressor MicroRNA and class I and II HDAC gene expression induce apoptosis in Glioblastoma cancer cells in vitro. Life Sci. 2021; 286:120024. https://doi.org/10.1016/j. lfs.2021.120024 DOI: https://doi.org/10.1016/j.lfs.2021.120024

Balakrishnan Purushothaman, Nagarasan Suganthi, Arunachalam Jothi, Kumaran Shanmugam. Molecular Docking Studies of potential anticancer agents from Ocimum basilicum L. against human colorectal cancer regulating genes: An in silico approach. Research J. Pharm. and Tech. 2019; 12(7):3423-7. https://doi.org/10.5958/0974-360X.2019.00579.1 DOI: https://doi.org/10.5958/0974-360X.2019.00579.1

Purushothaman, B., Suganthi, N., & Shanmugam, K. Qualitative and Quantitative Determination of Various Extracts of Ocimum basilicum L. Leaves. J. Nat. Rem. 2020; 20(1): 53-60. https://doi.org/10.18311/jnr/2020/24113 DOI: https://doi.org/10.18311/jnr/2020/24113

Sethuraman J, Nehru H, Shanmugam K, Balakrishnanan P. Evaluation of potent phytochemicals and antidiabetic activity of Ficus racemose L. W. J. Pharm. Res. 2017; 6(15): 909-20.

M JR, Ramalingam PS, Mathavan S, B R D Yamajala R, Moparthi NR, Kurappalli RK, Manyam RR. Synthesis, in vitro and structural aspects of cap substituted Suberoylanilide hydroxamic acid analogs as potential inducers of apoptosis in Glioblastoma cancer cells via HDAC /microRNA regulation. Chem Biol Interact. 2022; 357:109876. https://doi.org/10.1016/j.cbi.2022.109876 DOI: https://doi.org/10.1016/j.cbi.2022.109876

Whent M, Hao J, Slavin M, Zhou M, Song J, Kenworthy W, Yu LL. Effect of genotype, environment, and their interaction on chemical composition and antioxidant properties of low-linolenic soybeans grown in Maryland. J Agric Food Chem. 2009; 57(21):10163-74. https://doi.org/10.1021/ jf902344h DOI: https://doi.org/10.1021/jf902344h

Slavin M, Kenworthy W, Yu LL. Antioxidant properties, phytochemical composition, and antiproliferative activity of Maryland-grown soybeans with colored seed coats. J Agric Food Chem. 2009; 57(23):11174-85. https://doi. org/10.1021/jf902609n DOI: https://doi.org/10.1021/jf902609n

Agarwal DK, Billore SD, Sharma AN, et al. Soybean: Introduction, Improvement, and Utilization in India- Problems and Prospects. Agric Res. 2013; 2:293-300. https://doi.org/10.1007/s40003-013-0088-0 DOI: https://doi.org/10.1007/s40003-013-0088-0

Liu J, Chang SK, Wiesenborn D. Antioxidant properties of soybean isoflavone extract and tofu in vitro and in vivo. J Agric Food Chem. 2005; 53(6):2333-40. https://doi.org/10.1021/jf048552e DOI: https://doi.org/10.1021/jf048552e

Kerwin SM. Soy saponins and the anticancer effects of soybeans and soy-based foods. Curr Med Chem Anticancer Agents. 2004; 4(3):263-72. https://doi.org/10.2174/1568011043352993 DOI: https://doi.org/10.2174/1568011043352993

Tapiero H, Tew KD, Nguyen, BG, Mathe G. Polyphenols, do they play a role in the prevention of human pathologies? Biomed. Pharmacother. 2002; 56:200-7. https://doi.org/10.1016/S0753-3322(02)00178-6 DOI: https://doi.org/10.1016/S0753-3322(02)00178-6

Luthria DL, Biswas R, Natarajan S. Comparison of extraction solvents and techniques used for the assay of isoflavones from soybean. Food Chem. 2007; 105:325-33. https://doi. org/10.1016/j.foodchem.2006.11.047 DOI: https://doi.org/10.1016/j.foodchem.2006.11.047

Phommalath S, Teranishi M, Yoshikawa T, Saito H, Tsukiyama T, Nakazaki T, Tanisaka T, and Okumoto Y. Wide genetic variation in phenolic compound content of seed coats among black soybean cultivars Breed Sci. 2014; 64(4):409-15. https://doi.org/10.1270/jsbbs.64.409 DOI: https://doi.org/10.1270/jsbbs.64.409