Effect of Gymnema sylvestre Extract on the Regulation of AMPK-GLUT4 Mediated Signaling Pathway on Insulin Resistance in a PCOS Rat Model

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Authors

  • Arul Balasubramanian
     Department of Pharmacology, Vinayaka Mission’s College of Pharmacy, Vinayaka Mission’s Research Foundation (Deemed to be University), Salem - 636308, Tamil Nadu ,IN
  • Kothai Ramalingam
     Department of Pharmacology, Vinayaka Mission’s College of Pharmacy, Vinayaka Mission’s Research Foundation (Deemed to be University), Salem - 636308, Tamil Nadu ,IN
  • Sudhakar Pachiappan
     Department of Pharmacology, Vinayaka Mission’s College of Pharmacy, Vinayaka Mission’s Research Foundation (Deemed to be University), Salem - 636308, Tamil Nadu ,IN

DOI:

https://doi.org/10.18311/jnr/2023/29805

Keywords:

Gymnema sylvestre, Insulin Resistance, Letrozole, Metformin, Polycystic Ovary Syndrome
Reproductive Pharmacology

Abstract

This study was intended to assess the effect of Gymnema sylvestre leaf extract on the AMPK-GLUT4 mediated signalling pathway on insulin resistance in polycystic ovarian syndrome-induced rats. For the induction of PCOS in rats, 1 mg/kg of letrozole was given orally for 21 days, after that metformin (300 mg/kg), Gymnema sylvestre leaf extract (200 mg/kg, and 400 mg/kg p. o.) were administered for further 28 days. Letrozole-induced PCOS rats illustrated significant estrus irregularity, and sex hormonal abnormality, and developed insulin resistance as indicated by increased fasting glucose levels, an increased rate of glucose clearance, and also decreases in GLUT4 and AMPK mRNA expression in ovarian cells. Ovarian histology in PCOS rats also showed many follicular cysts, atretic follicles, and the absence of the corpus luteum. These changes were significantly reversed by the treatment of G. sylvestre in a dose-dependent manner. It might be mediated through its insulin resistance modulating property by the activation of AMPK & GLUT4 expression on ovarian endometrial cells.

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Published

2023-05-23

How to Cite

Balasubramanian, A., Ramalingam, K., & Pachiappan, S. (2023). Effect of <i>Gymnema sylvestre</i> Extract on the Regulation of AMPK-GLUT4 Mediated Signaling Pathway on Insulin Resistance in a PCOS Rat Model. Journal of Natural Remedies, 23(2), 429–439. https://doi.org/10.18311/jnr/2023/29805

Issue

Volume 23, Issue 2, April 2023

Section

Research Articles

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Copyright (c) 2022 Sudhakar Pachiappan, Kothai Ramalingam, Arul Balasubramanian

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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Received 2022-03-15
Accepted 2022-12-13
Published 2023-05-23

 

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