In Vitro Antioxidant and Anticancer Activity of Plant Extracts of Tanacetum dolicophyllum (Kitam.) Kitam

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Authors

  • Gunjan Sharma
     School of Applied Sciences, Suresh Gyan Vihar University, Jaipur – 302017, Rajasthan ,IN
  • Zoya Zaidi
     Synthetic Organic Chemistry Research Lab, Department of Chemistry, Thanthai Hans Roever College, Perambalur – 621212, Tamil Nadu ,IN
  • Mohammad Irfan Ali
     Faculty of Life Sciences, Mandsaur University, Mandsaur – 458001, Madhya Pradesh ,IN
  • Sarmad Moin
     School of Applied Sciences, Suresh Gyan Vihar University, Jaipur – 302017, Rajasthan ,IN

DOI:

https://doi.org/10.18311/jnr/2023/30460

Keywords:

Alkaloids, Anticancer, Antioxidant, Cytotoxicity, Tanacetum dolicophyllum

Abstract

The objective of this study was the assessment of antioxidant and cytotoxic activity from Tanacetum dolicophyllum. Soxhlet extraction has been used for preparing plant extracts for which five solvents have been used, i.e., Petroleum ether, benzene, ethyl acetate, ethanol, and water. Proteins, tannins, phlobatannins, amino acids, carbohydrates, alkaloids, steroids, flavonoids, glycosides, cardiac glycosides, and phenols were detected in the sample extracts. In the phytochemical screening of plant extracts, ethyl acetate and ethanolic extract show the best results which further leads to the investigation of antioxidant activity. Ferric-Reducing Antioxidant Power (FRAP), 2,2-diphenyl-2-picrylhydrazyl (DPPH), Reducing Power assay, and Phosphomolybdenum assays were performed to estimate antioxidant activity. In the RPA and TAC, the absorbance of sample extracts increases as there is an increase in concentration. In DPPH and FRAP assay, ethyl acetate showed good results. DPPH assay for ethyl acetate extract with 172.73(µg/ml) and ethanolic extract with 171.07(µg/ml) of IC50 values. The ethyl acetate extract had the highest antioxidant activity. The MTT assay was used to investigate the anticancer activity. The MTT assay discovered that the ethyl acetate extract had the highest anticancer activity against HeLa cells with an IC50 75 µg/mL as compared to a normal cell line J774A. Several compounds present in the extract of ethyl acetate acted as anticancer and contributed to cytotoxic activity.

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Published

2023-06-13

How to Cite

Sharma, G., Zaidi, Z., Ali, M. I., & Moin, S. (2023). <i>In Vitro</i> Antioxidant and Anticancer Activity of Plant Extracts of <i>Tanacetum dolicophyllum</i> (Kitam.) Kitam. Journal of Natural Remedies, 23(2), 455–465. https://doi.org/10.18311/jnr/2023/30460

Issue

Volume 23, Issue 2, April 2023

Section

Research Articles

License

Copyright (c) 2023 Gunjan Sharma, Zoya Zaidi, Mohammad Irfan Ali, Sarmad Moin

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Europe PMC
Received 2022-06-13
Accepted 2023-04-25
Published 2023-06-13

 

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