Green Synthesis of Silver Nanoparticles using Lantana camara Leaves Extract and In Vitro Evaluation of Oral Anti-cancer Activity

Abstract

At present, green synthesized based drug delivery systems play a major role in the bio medical field. Lantana camara, also known as Unni chedi in Tamil, is an evergreen shrub that is involved in a variety of cancer diseases due to its pharmacological activity. In addition, the leaf extract of Lantana camara was found to have excellent anti cancer activity. The green synthesized AgNPs of Lantana camara leaves extract were more efficient against oral cancer. In this report, green synthesized nano-formulations in presence of AgNPs were formulated. The as prepared formulation was confirmed by UV spectroscopy, particle size and zeta potential, Scanning Electron Microscopy (SEM). In UV Spectroscopy the absorbance of the peak appears between the range of 200 to 300 nm corresponding to the formulation of AgNPs. The Zeta potential shows high colloidal stability; the average potential value is -12.6mV. The particle size results shown that the average size of AgNPs is 268.1 nm. The morphology and particle size determined using SEM analysis indicate spherical shaped particles. Further, the oral anti-cancer activity was evaluated on Squamous Cell Cancer (SCC-25) cell line. The cell line result shown is that the IC 50 value for AgNPs was 39µg/ml and the plant extract shown 145µg/ml, this indicates that the silver nanoparticles have more potential when compared to the leaf extract.