Anti-bacterial and Anti-fungal Activities of Various Extracts of Acanthospermum hispidum DC

Abstract

Objective: To examine the 50% aqueous-ethanol, benzene and chloroform extracts of whole plant of Acanthospermum hispidum DC for their anti-bacterial and anti-fungal properties against a wide range of microorganisms. Method: The anti-bacterial activity was studied by agar dilution method using Muller Hinton (MH) agar media. The results were compared with standard ciprofloxacin (5 μg / disc). The anti-fungal activity of the extracts was investigated by tube dilution method using Sabouraud Dextrose Agar (SDA) medium and the results compared with standard Clotrimazole (125 μg/ml). Result: All studied extracts showed potential anti-bacterial and anti-fungal properties. The MIC (Minimum Inhibitory Concentration) was found to be 66.66 μg / ml for benzene extract and of varying range (66.66-200 μg / ml) for chloroform and 50% aqueous-ethanol extracts, with different micro-organisms. The anti-fungal property of all the studied extracts were comparable with that of standard clotrimazole. Conclusion: The present study brought to light the scientific data on the anti-infective property of the plant.