Simultaneous Determination of Tannic Acid and Eugenol in Newly Formulated Churna of Tulsi and Parijat

Jump To References Section

Authors

  • Hiral Dave
     Department of Pharmaceutical Quality Assurance, Parul Institute of Pharmacy, Parul University, Waghodia, Vadodara - 391760, Gujarat. ,IN
  • Krupali Shukla
     Department of Pharmaceutical Quality Assurance, Parul Institute of Pharmacy, Parul University, Waghodia, Vadodara - 391760, Gujarat. ,IN

DOI:

https://doi.org/10.18311/jnr/2023/31906

Keywords:

Churna, Herbal Formulation, High-Performance Thin Layer Chromatography, Immunomodulators, Phytoconstituents, Standardization of Herbs.

Abstract

Herbal formulations are widely used to treat a variety of diseases with the use of natural ingredients and have the additional advantage of negligible side effects. Looking towards the wide applicability of herbal formulations, a novel herbal formulation containing Tulsi and Parijat has been formulated and evaluated for different standardisation parameters. Tulsi and Parijat both possess multiple natural effects like antibacterial, antiviral and antipyretic properties along with wide applicability to treat different respiratory problems. Therefore, the combination of these two naturally available herbs can provide good immunomodulatory action for humans. Apart from the various phytoconstituents present in these two herbs, eugenol and tannic acid are found to have versatile effects. They are widely used to treat various common health issues like tonsillitis and various respiratory complaints like cough, cold and sour throat. A successful attempt has been made to develop the HPTLC method for the simultaneous determination of Tannic acid and Eugenol in pure and newly formulated churna containing Tulsi and Parijat as chief herbs. Tannic acid and eugenol mixtures get resolved by toluene:ethyl acetate:formic acid (5:3:2) with Rf of 0.849 ± 0.003 and 0.534 ± 0.009 respectively for tannic acid and eugenol. Linearity is observed within the range of 250–1500 ng/band for both eugenol and tannic acid. According to ICH Q2 R1 advice for analytical method validation, this designed technique has been effectively validated.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Downloads

Published

2023-03-23

How to Cite

Dave, H., & Shukla, K. (2023). Simultaneous Determination of Tannic Acid and Eugenol in Newly Formulated Churna of Tulsi and Parijat. Journal of Natural Remedies, 23(1), 223–230. https://doi.org/10.18311/jnr/2023/31906

Issue

Volume 23, Issue 1, January 2023

Section

Research Articles

License

Copyright (c) 2023 Dr Hiral Dave, Krupali Shukla (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Crossref
Scopus
Google Scholar
Europe PMC
Received 2022-11-10
Accepted 2023-03-23
Published 2023-03-23

 

References

Pal SK, Shukla Y. Herbal medicine: current status and the future. Asian Pacific Journal of Cancer Prevention. 2003; 4(4):281-8.

P. R. V. India Herbal drugs and formulations. International Conference and Exhibition on Pharmacognosy and Natural Products. 2013; 2:4-5.

Agarwal P, Nagesh L. Evaluation of the antimicrobial activity of various concentrations of Tulsi (Osmium sanctum) extract against Streptococcus mutans: An in vitro study. Ind J of Dental Res. 2010; 21(3):257-9. https://doi. org/10.4103/0970-9290.70800 DOI: https://doi.org/10.4103/0970-9290.70800

Upadhyay RK. Tulsi: A holy plant with high medicinal and therapeutic value. Int J of Green Pharmacy. 2017; 11(01):S1- S12.

PubChem. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; 2004. PubChem Compound Summary for CID 3314, Eugenol.

PubChem. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; 2004. PubChem Compound Summary for CID 16129778, Tannic acid.

Hussain SZ, Maqbool K, Naseer B. High-Performance Thin Layer Chromatography: Principle, working and applications. Int J Res Pharm Pharm Sci. 2019; 4:83-89.

Sonia K, Lakshmi KS. HPTLC method development and validation: An overview. Journal of Pharmaceutical Sciences and Research. 2017; 9(5):52-59.

Trivedi PD, Kilambi P. A Validated High-Performance Thin Layer Chromatographic method for the estimation of gym emic acids through gymnemagenin in Gymnemate Sylvester, materials, extracts and formulations. Int. J. App. Sci. and Engg. 2007; 6(1):19-28.

Ayurvedic pharmacopoeia India part–II volume–I of Preparation and standardization of Churna.

International Conference on Harmonization (ICH) of Technical Requirements for the Registration of Pharmaceutical for Human Use, Validation of Analytical Procedures: Text and Methodology Q2 (R1). 2005; 1–13.

Dave HN, Makwana AG, Suhagia BN. Validated HPTLC method for the determination of two novel steroids in bulk and pressurized metered - dose preparations. Int. J. App. Sci. and Engg. 2011; 9(3):177-85.