In Vitro Pharmacological Evaluation of Fulvic Acid and Humic Acid Novel Combination in Amyloid Beta Intoxicated SH-SY5Y Cell Lines Model of Alzheimer’s Disease

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Authors

  • Thamotharan Govindhasamy
     Department of Pharmaceutical Chemistry, Vinayaka Mission’s College of Pharmacy, Salem - 636008, Tamil Nadu, India ,IN
  • Kumar Mohan
     Department of Pharmaceutical Chemistry, Vinayaka Mission’s College of Pharmacy, Salem - 636008, Tamil Nadu, India ,IN

DOI:

https://doi.org/10.18311/jnr/2023/32992

Keywords:

Alzheimer’s Disease, Cytokines, Dementia, Fulvic Acid, Humic Acid, Neurodegeneration

Abstract

Alzheimer’s disease is the most widespread advancing neurodegenerative disorder, and it is estimated that more than 50 million people have been affected worldwide. It is characterized by dementia, loss of sensory and motor function and difficulty in performing work. NMDA antagonists and acetylcholinesterase inhibitors are currently being used as treatment options for AD, but the treatment would not decline the disease progression as well as neurodegeneration. We used a unique humic acid and Fulvic acid mixture in the current investigation for the treatment of amyloid-β intoxicated Alzheimer’s in vitro model in SH-SY5Y cell line. Humic acid and fulvic acids are organic substances which will be produced by microorganism from the biomass such as dead matter of animal as well as plants. Both the components were used in the 1:1 ratio against AD induced cell line with optimum concentration 50μM/ml of each compound. The inflammatory mediators like Reactive Oxygen Species (ROS) were estimated using flow cytometry - H2DCFDA staining assay and cytokines such as TNF-α and IL-1β levels were estimated using ELISA. The results obtained from present study implicating that the humic acid and fulvic acids and their novel combinations lessen the ROS, cytokines level and decreased the apoptosis levels there by it exhibiting the neuro-protective mechanism via inhibiting neuro-inflammatory pathway in AD.

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Published

2023-11-05

How to Cite

Govindhasamy, T., & Mohan, K. (2023). <i>In Vitro</i> Pharmacological Evaluation of Fulvic Acid and Humic Acid Novel Combination in Amyloid Beta Intoxicated SH-SY5Y Cell Lines Model of Alzheimer’s Disease. Journal of Natural Remedies, 23(4), 1437–1448. https://doi.org/10.18311/jnr/2023/32992

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Volume 23 Issue 4 October 2023

Section

Research Articles

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Copyright (c) 2022 Thamotharan Govindhasamy, Kumar Mohan (Author)

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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Europe PMC
Received 2023-02-12
Accepted 2023-08-31
Published 2023-11-05

 

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