Development and Greenness Assessment of Analytical Quality by Design Optimised Eco-friendly UV Spectrophotometric Methods for Analysis of Two Natural Antioxidants in Pure and Formulation

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Authors

  • Ramya Jonnalagadda
     Department of Pharmaceutical Chemistry, Sri Ramachandra Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai - 600116, Tamil Nadu ,IN
  • Seetharaman Rathinam
     Department of Pharmaceutical Analysis, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur - 603203, Tamil Nadu ,IN
  • Vinodhini Chandrasekar
     Department of Pharmaceutical Chemistry, Sri Ramachandra Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai - 600116, Tamil Nadu ,IN

DOI:

https://doi.org/10.18311/jnr/2023/33662

Keywords:

Analytical Eco-scale, Analytical Greenness Metrics, Analytical Quality by Design, Curcumin, Green Analytical Procedure Index, Silybin

Abstract

Utilizing analytical quality by design and green analytical chemistry principles, the present work introduces simple, robust, and environmentally benign UV methods. Two separate spectrophotometric methods were developed for the estimation of Silybin and Curcumin, where solvent, scan speed, and sampling interval are the estimated critical parameters. The detection was carried out at absorption maxima of 288nm for Silybin and 419nm for Curcumin with ethanol. To determine the critical method variables, a risk assessment was carried out using an Ishikawa diagram. Developed spectrophotometric methods were validated according to the International Council for Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use Q2 (R1) guidelines. The proposed methods showed good predictability and robustness. The new methodologies were found to be green according to the analytical greenness metric approach and software, the green analytical procedure index, and analytical eco-scale tools in comparison to the existing methods.

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Author Biographies

Seetharaman Rathinam, Department of Pharmaceutical Analysis, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur - 603203, Tamil Nadu

Department of Pharmaceutical Analysis

Vinodhini Chandrasekar, Department of Pharmaceutical Chemistry, Sri Ramachandra Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai - 600116, Tamil Nadu

Department of Pharmaceutical Chemistry

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Published

2023-11-08

How to Cite

Jonnalagadda, R., Rathinam, S., & Chandrasekar, V. (2023). Development and Greenness Assessment of Analytical Quality by Design Optimised Eco-friendly UV Spectrophotometric Methods for Analysis of Two Natural Antioxidants in Pure and Formulation. Journal of Natural Remedies, 23(4), 1475–1490. https://doi.org/10.18311/jnr/2023/33662

Issue

Volume 23 Issue 4 October 2023

Section

Research Articles

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Copyright (c) 2022 Ramya Jonnalagadda, Seetharaman Rathinam, Vinodhini Chandrasekar (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Europe PMC
Received 2023-04-29
Accepted 2023-07-24
Published 2023-11-08

 

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