Evaluation of Neuroprotective Effect of Salicin in an Experimental Animal Model of Streptozotocin Induced Diabetic Neuropathy

Jump To References Section

Authors

  • Ghanshayam B. Jadhav
     Department of Pharmacology, NDMVP College of Pharmacy, Nashik – 422005, Maharashtra ,IN
  • Shubham J. Khairnar
     Department of Pharmacology, NDMVP College of Pharmacy, Nashik – 422005, Maharashtra ,IN
  • Simona E. D'Souza
     Department of Pharmacology, MET’s Institute of Pharmacy, Nashik – 422003, Maharashtra ,IN
  • Pavan B. Udavant
     Department of Pharmacology, MET’s Institute of Pharmacy, Nashik – 422003, Maharashtra ,IN

DOI:

https://doi.org/10.18311/jnr/2024/35277

Keywords:

Antioxidant, Oxidative Stress, Peripheral Neuropathy, Polyphenols, Proinflammatory Mediators, Reactive Oxygen Species

Abstract

Background: Diabetic neuropathy stands as the most prevalent secondary complication connected with diabetes mellitus. The susceptibility of mammalian nerves to oxidative stress is heightened due to their rich phospholipid content, leading to a reduced ability to counteract the free radicals responsible for neuropathy. While synthetic treatments can help alleviate oxidative stress, they often come with unwanted side effects. Shifting the focus towards natural pharmaceuticals could mitigate these negative effects. Phenolic compounds abundant in antioxidants may aid in reducing oxidative stress. Aim: Assess the preventive influence of Salicin, a phenolic compound against diabetic neuropathy induced by Streptozotocin (STZ). Methods: Four weeks following the injection of STZ into the peritoneal cavity, a noticeable reduction in thermal and mechanical hyperalgesia, cold allodynia, motor coordination and locomotor activity was noted. Natural antioxidants such as reduced glutathione and catalase were assessed along with lipid peroxidation levels on the 28th day and the sciatic nerve was subjected to histopathological examination. Results: Orally administering Salicin at dosages of 10, 15, and 20mg/kg over 28 days successfully mitigated the reduction in the nociceptive threshold and bolstered the levels of endogenous antioxidants. It also mitigated the unwanted histopathological changes effectively based on the dosage. Conclusion: Salicin having antioxidant properties, demonstrates potential in alleviating diabetic neuropathic pain and preventing associated complications.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Downloads

Published

2024-06-30

How to Cite

Jadhav, G. B., Khairnar, S. J., D’Souza, S. E., & Udavant, P. B. (2024). Evaluation of Neuroprotective Effect of Salicin in an Experimental Animal Model of Streptozotocin Induced Diabetic Neuropathy. Journal of Natural Remedies, 24(6), 1273–1287. https://doi.org/10.18311/jnr/2024/35277

Issue

Volume 24, Issue 6, June 2024

Section

Research Articles

Categories

License

Copyright (c) 2024 Ghanshayam B. Jadhav, Shubham Khairnar, Simona E. D'Souza, Pavan B. Udavant, Naveed R. Ahmad (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Crossref
Scopus
Google Scholar
Europe PMC
Received 2023-10-05
Accepted 2024-04-24
Published 2024-06-30

 

References

Ibrahim MA, Abdelzaher WY, Rofaeil RR, Abdelwahab S. Efficacy and safety of combined low doses of either diclofenac or celecoxib with gabapentin versus their single high dose in treatment of neuropathic pain in rats. Biomed. Pharmacother. 2018; 100:267-74. https://doi.org/10.1016/j.biopha.2018.01.102 PMid:29438840.

Mahomoodally MF, MA-L ER. Catalase. In Antioxidants Effects in Health. Elsevier. 2022. p. 81-90. https://doi.org/10.1016/B978-0-12-819096-8.00022-7

Singh R, Kishore L, Kaur N. Diabetic peripheral neuropathy: current perspective and future directions. Pharmacol Res.2014; 80:21-35. https://doi.org/10.1016/j.phrs.2013.12.005 PMid:24373831.

Kartha S, Yan L, Weisshaar CL, Ita ME, Shuvaev VV, Muzykantov VR, Tsourkas A, Winkelstein BA, Cheng Z. Superoxide dismutase‐loaded porous polymersomes as highly efficient antioxidants for treating neuropathic pain. Adv. Healthc. Mater. 2017; 6(17):1700500. https://doi.org/10.1002/adhm.201700500 PMid:28671302 PMCid: PMC5591629.

Junejo JA, Zaman K, Rudrapal M, Celik I, Attah EI. Antidiabetic bioactive compounds from Tetrastigma angustifolia (Roxb.) Deb and Oxalis debilis Kunth: Validation of ethnomedicinal claim by in vitro and in silico studies. S AFR J BOT. 202; 143:164-75. https://doi.org/10.1016/j.sajb.2021.07.023

Paul A, Kumar M, Das P, Guha N, Rudrapal M, Zaman MK. Drug repurposing- A search for novel therapy for the treatment of diabetic neuropathy. Biomed Pharmacother. 2022; 156:113846. https://doi.org/10.1016/j.biopha.2022.113846 PMid:36228378.

Hussain N, Kakoti BB, Rudrapal M, Sarwa KK, Celik I, Attah EI, Khairnar SJ, Bhattacharya S, Sahoo RK, Walode SG. Bioactive antidiabetic flavonoids from the stem bark of Cordia dichotoma Forst.: Identification, docking and ADMET studies. Molbank. 2021; 2021(2):M1234. https://doi.org/10.3390/M1234

Rao BS, Reddy KE, Parveen K, Narendra BL, Shekhar SC, Mangala L. Effects of Cleome viscosa on hyperalgesia, oxidative stress and lipid profile in STZ induced diabetic neuropathy in Wistar rats. Pak J Pharm Sci. 2014; 27(5):1137-45.

Oyenihi AB, Ayeleso AO, Mukwevho E, Masola B. Antioxidant strategies in the management of diabetic neuropathy. Biomed Res Int. 2015; 2015:515042. https://doi.org/10.1155/2015/515042 PMid:25821809 PMCid: PMC4363503.

Junejo JA, Zaman K, Ali M, Rudrapal M. New flavonoid with antidiabetic and antioxidant potential from Tetrastigma angustifolia (Roxb.) Deb leaves. Braz J Pharm Sci. 2021; 56:e18806. https://doi.org/10.1590/s2175-97902019000418806

Junejo JA, Mondal P, Verma VK, Rudrapal M, Zaman MK. Anti-diabetic assessment of the hydro-alcoholic leaf extracts of the plant Tetrastigma angustifolia (Roxb.) a traditionally used North-Eastern Indian vegetable. Biomed Pharmacol J. 2014; 7(2):635. https://doi.org/10.13005/bpj/535

Junejo JA, Rudrapal M, Nainwal LM, Zaman K. Antidiabetic activity of hydro-alcoholic stem bark extract of Callicarpa arborea Roxb. with antioxidant potential in diabetic rats. Biomed Pharmacother. 2017; 95:84-94. https://doi.org/10.1016/j.biopha.2017.08.032 PMid:28826101.

Khairnar S, Pawar S, Patil V, Rudrapal M. Effect of vanillic acid in streptozotocin-induced diabetic neuropathy. Asian J Biol Life Sci. 2020; 9:306-12. https://doi.org/10.5530/ ajbls.2020.9.46

Sharma S, Kulkarni SK, Agrewala JN, Chopra K. Curcumin attenuates thermal hyperalgesia in a diabetic mouse model of neuropathic pain. Eur J Pharmacol. 2006; 536(3):256-61. https://doi.org/10.1016/j.ejphar.2006.03.006 PMid:16584726.

Chen L, Chen W, Qian X, Fang Y, Zhu N. Liquiritigenin alleviates mechanical and cold hyperalgesia in a rat neuropathic pain model. Sci Rep. 2014; 4(1):5676. https://doi.org/10.1038/ srep05676 PMid:25022218 PMCid:PMC4097342.

Song YN, Li HZ, Zhu JN, Guo CL, Wang JJ. Histamine improves rat rota-rod and balance beam performances through H2 receptors in the cerebellar interpositus nucleus. Neurosci. 2006; 140(1):33-43. https://doi.org/10.1016/j.neuroscience.2006.01.045 PMid:16533576.

Allchorne AJ, Broom DC, Woolf CJ. Detection of cold pain, cold allodynia and cold hyperalgesia in freely behaving rats. Mol Pain. 2005; 1:1744-8069. https://doi.org/10.1186/17448069-1-36 PMid:16354295 PMCid:PMC1325266.

Zhu YJ, Zeng T, Zhu YB, Yu SF, Wang QS, Zhang LP, Guo X, Xie KQ. Effects of acrylamide on the nervous tissue antioxidant system and sciatic nerve electrophysiology in the rat. Neurochem Res. 2008; 33:2310-7. https://doi.org/10.1007/s11064-008-9730-9 PMid:18470611.

Khan TH, Sultana S. Antioxidant and hepatoprotective potential of Aegle marmelos Correa. against CCl4induced oxidative stress and early tumour events. J Enzyme Inhib Med Chem. 2009; 24(2):320-7 .https://doi.org/10.1080/14756360802167754 PMid:18830880.

Kaur G, Bedi O, Sharma N, Singh S, Deshmukh R, Kumar P. Anti-hyperalgesic and anti-nociceptive potentials of standardised grape seed proanthocyanidin extract against CCI-induced neuropathic pain in rats. J Basicclin Physiol Pharmacol. 2016; 27(1):9-17. https://doi.org/10.1515/ jbcpp-2015-0026 PMid:26378488.

Mondal S, Ghosh D, Ganapaty S, Chekuboyina SV, Samal M. Hepatoprotective activity of Macrothelypteris torresiana (Gaudich.) aerial parts against CCl4-induced hepatotoxicity in rodents and analysis of polyphenolic compounds by HPTLC. J Pharm Res. 2017; 7(3):181-9. https://doi.org/10.1016/j.jpha.2016.12.001 PMid:29404036 PMCid: PMC5790686.

Uzar E, Alp H, Cevik MU, Fırat U, Evliyaoglu O, Tufek A, Altun Y. Ellagic acid attenuates oxidative stress on the brain and sciatic nerve and improves histopathology of the brain in streptozotocin-induced diabetic rats. Neuro Sci. 2012; 33:567-74. https://doi.org/10.1007/s10072-011-0775-1 PMid:21922312.

Ševců A, El-Temsah YS, Joner EJ, Černík M. Oxidative stress induced in microorganisms by zero-valent iron nanoparticles. Environ Microbial. 2011; 26(4):271-81. https://doi.org/10.1264/jsme2.ME11126 PMid:21791884.

Nayak Y, Hillemane V, Daroji VK, Jayashree BS, Unnikrishnan MK. Antidiabetic activity of benzopyrone analogues in nicotinamide-streptozotocin induced type 2 diabetes in rats. Sci World J. 2014. p. 854267. https://doi.org/10.1155/2014/854267 PMid:25548795 PMCid: PMC4274851.

Oghbaei H, Mohaddes G, Hamidian G, Keyhanmanesh R. Sodium nitrate preconditioning prevents progression of the neuropathic pain in streptozotocin-induced diabetes Wistar rats. J Diab Metabol Dis. 2020; 19:105-13. https://doi.org/10.1007/s40200-019-00481-4 PMid:32550160 PMCid: PMC7270373.

Preshant T, Bimlesh K, Mandeep K, Gurpreet K, Harleen K. Phytochemical screening and extraction: A review. Inter Pharmaceutica Sciencia. 2011; 1(1):98-108.

Nandi A, Yan LJ, Jana CK, Das N. Role of catalase in oxidative stress-and age-associated degenerative diseases. Oxid Med Cell Longev. 2019; 2019:9613090. https://doi.org/10.1155/2019/9613090 PMid:31827713 PMCid: PMC6885225.