Evaluation of Prebiotic Potential and HPTLC Analysis of Withania somnifera with Application in Improving the Chemotherapy-induced GI Side Effects

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Authors

  • Rashmi C. Yadav
     Department of Pharmacognosy, Dr. DY Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Chinchwad – 411018, Maharashtra ,IN
  • Santosh S. Bhujbal
     Department of Pharmacognosy, Dr. DY Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Chinchwad – 411018, Maharashtra ,IN ORCID logo https://orcid.org/0000-0002-2801-5955
  • Aarti Supekar
     Department of Pharmacognosy, Dr. DY Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Chinchwad – 411018, Maharashtra ,IN

DOI:

https://doi.org/10.18311/jnr/2024/35804

Keywords:

Gut Microbiota, HPLTC, Lactobacillus acidophilus, Prebiotic, Withania somnifera

Abstract

Background: Chemotherapy is a known choice of treatment for Cancer which has the aspect of producing strong adverse effects affecting the quality of life. To apar these various options are available wherein prebiotic use is emerging. An important herb Ashwagandha, derived from the dried mature roots of Withania somnifera (WS) Dunal, from the Solanaceae family (genus Withania), is utilised as a study specimen. Aim: The present study focuses on the determination of the prebiotic potential of the WS as a growth stimulant using Lactobacillus acidophilus (LA) with the support of HPTLC fingerprinting and marker study. Method: The growth analysis of WS was done using LA under microaerophilic conditions for 48hrs. The activity of WS is being confirmed using HPTLC m fingerprinting and marker study. Results: The extract showed the presence of the withaferin A with Rf of 0.364 using a solvent system (Methanol: Ethylacetate: Formic acid (1:9:0.5) and confirmed with standard marker using a solvents system of (Toluene: Ethyl acetate: Methanol: GAA (5:3.5:1:0.5). Conclusion: The growth of LA is stimulated showing an increase in lactic acid concentration and pH. HPTLC fingerprinting and marker study showed the presence of desired withanolides.

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Published

2024-06-30

How to Cite

Yadav, R. C., Bhujbal, S. S., & Supekar, A. (2024). Evaluation of Prebiotic Potential and HPTLC Analysis of <i>Withania somnifera</i> with Application in Improving the Chemotherapy-induced GI Side Effects. Journal of Natural Remedies, 24(6), 1289–1295. https://doi.org/10.18311/jnr/2024/35804

Issue

Volume 24, Issue 6, June 2024

Section

Research Articles

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Copyright (c) 2024 Rashmi C. Yadav, Santosh S. Bhujbal, Aarti Supekar (Author)

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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Received 2023-12-06
Accepted 2024-05-10
Published 2024-06-30

 

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