Extracts of Selected Plants and Fruits Profoundly Modulate In Vitro Coagulation Activity

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Authors

  • Adenike O. Oladokun
     Medical Laboratory Science Programme, College of Health Sciences, Bowen University, Iwo, Osun State ,NG
  • Eziuche V. Ibe
     Medical Laboratory Science Programme, College of Health Sciences, Bowen University, Iwo, Osun State ,NG
  • Faridah Ismail
     Medical Laboratory Science Programme, College of Health Sciences, Bowen University, Iwo, Osun State ,NG
  • Gbenga T. Ajayi
     Medical Laboratory Science Programme, College of Health Sciences, Bowen University, Iwo, Osun State ,NG
  • Olufemi E. Akanni
     Department of Medical Laboratory Science, College of Health Sciences, Osun State University, Osogbo, Osun State ,NG
  • Oduola O. Abiola
     Medical Laboratory Science Programme, College of Health Sciences, Bowen University, Iwo, Osun State ,NG

DOI:

https://doi.org/10.18311/jnr/2024/35928

Keywords:

Activated Partial Thromboplastin Time, Ananas comosus, Mangifera indica, Moringa oleifera, Prothrombin Time, Vernonia amygdalina

Abstract

Background: Plant products treat many diseases and are an important resource for pharmaceutical companies developing new medicines. Hemostasis maintains blood in the fluid state and prevents its loss from damaged blood vessels. Plants have been reported to be sources of anticoagulant and antithrombotic substances and are employed in the management of coagulation disorders. Aim: This study investigates the effects of aqueous and ethanolic extracts of bitter leaves, mango leaves, drumstick leaves, pineapples and tomatoes on in vitro coagulation activity. Methods: Aqueous and ethanolic extracts of leaves of Mangifera indica, Moringa oleifera, Vernonia amygdalina, and fruits of Ananas comosus and Solanum lycopersicum were prepared using the cold maceration method and lyophilized. The freeze-dried extracts were prepared into four concentrations. Platelet-poor plasma was obtained from ten healthy volunteers. Prothrombin Time (PT) and activated Partial Thromboplastin Time (aPTT) were determined by visual detection of clot formation. The mean and standard deviation were determined and compared with the controls. Results: PT values for females were higher for aqueous and ethanolic extracts except for A. comosus. Female aPTT values were reduced compared to males except for V. amygdalina. There were no changes in PT and aPTT across the concentration gradient. PT control values significantly differ, however, there were no significant differences in aPTT control values between genders. This study revealed that PT and aPTT values differ significantly between genders. Conclusion: Mangifera indica and M. oleifera have procoagulant activity, A. comosus has anticoagulant activity, while V. amygdalina does not affect coagulation time.

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Published

2024-11-06

How to Cite

Oladokun, A. O., Ibe, E. V., Ismail, F., Ajayi, G. T., Akanni, O. E., & Abiola, O. O. (2024). Extracts of Selected Plants and Fruits Profoundly Modulate <i>In Vitro</i> Coagulation Activity. Journal of Natural Remedies, 24(10), 2325–2337. https://doi.org/10.18311/jnr/2024/35928

Issue

Volume 24, Issue 10, October 2024

Section

Short Communication

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Copyright (c) 2024 Adenike O. Oladokun, Eziuche V. Ibe, Faridah Ismail, Gbenga T. Ajayi, Olufemi E. Akanni, Oduola O. Abiola (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Received 2023-12-18
Accepted 2024-06-10
Published 2024-11-06

 

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