Influence of Storage Methods of Whole Blood Samples on DNA Integrity in Epidemiological Studies

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Authors

  • C. Rahila
     Department of Public Health Dentistry, Vivekanandha Dental College and Hospital for Women, Tiruchengode, 637205 ,IN
  • M. B. Aswath Narayanan
     Department of Public Health Dentistry, Tamilnadu Government Dental College and Hospital, Chennai 600003 ,IN
  • J. John Kirubaharan
     Department of Veterinary Microbiology, Madras Veterinary College, Chennai 600007 ,IN
  • S .G. Ramesh Kumar
     Department of Public Health Dentistry, Tamilnadu Government Dental College and Hospital, Chennai 600003 ,IN
  • A. Leena Selvamary
     Department of Public Health Dentistry, Tamilnadu Government Dental College and Hospital, Chennai 600003 ,IN
  • A. Sujatha
     Department of Public Health Dentistry, Tamilnadu Government Dental College and Hospital, Chennai 600003 ,IN

Keywords:

DNA isolation, Genetic epidemiology, Whole blood
Dental Public Health

Abstract

Objectives: To investigate the influence of storage methods of whole blood samples on Deoxyribonucleic acid (DNA) quality.
Method and materials: Whole blood samples were collected from 90 patients reported to the Government Tertiary-care Dental College and Hospital, Tamil Nadu, India. The blood samples were stored at three different temperatures -80 °C, -20 °C, 4 °C which were analyzed for DNA quality immediately within 4 h and after a storage period of 60 days. Quantification of DNA in the sample was recorded using a spectrophotometer..
Results: The average concentration of isolated DNA ranged from 0.5 ng/μl to 154.2 ng/μl with purity A260/A280ratio ranging from 0.7 to 2.0. The mean DNA concentration H = 97.2 (p = 0.001) and purity H = 80.1 (p = 0.001) obtained from whole blood stored at 4 °C were statistically significant. Thus samples stored at 4 °C did not affect on DNA yield and quality..
Conclusion: We conclude that storage at 4 8C temperature for whole blood does not substantially affect the molecular assay measurements. This study enlightens the influence of storage temperatures and duration in genetic epidemiology.

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Published

2018-09-18

How to Cite

Rahila, C., Narayanan, M. B. A., Kirubaharan, J. J., Kumar, S. .G. R., Selvamary, A. L., & Sujatha, A. (2018). Influence of Storage Methods of Whole Blood Samples on DNA Integrity in Epidemiological Studies. Journal of Pierre Fauchard Academy (India Section), 31(2-4), 89–92. Retrieved from https://informaticsjournals.co.in/index.php/jpfa/article/view/21123

Issue

Volume 31, Issue 2-4, June - December 2017

Section

Original Articles

License

All the articles published in JPFA are distributed under a creative commons license. The journal allows the author(s) to hold the copyright of their work (all usages allowed except for commercial purpose).

 

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