Comparative Evaluation of Anticandidal Activity of Pre-incorporated Quaternary Ammonium Compound Disinfectant Alginate With 5.25% Sodium Hypochlorite Spray Disinfectant on the Conventional Alginate: An In Vivo Study

Jump To References Section

Authors

  • Harekrishna J. Raval
     Consultant Prosthodontist, Ahmedabad, Gujarat ,IN
  • Neerja Mahajan
     Department of Prosthodontics, K M Shah Dental College and Hospital, Sumandeep Vidyapeeth, Piparia, Waghodia, 391760 ,IN
  • Rajesh Sethuraman
     Department of Prosthodontics, K M Shah Dental College and Hospital, Sumandeep Vidyapeeth, Piparia, Waghodia, 391760 ,IN
  • Naveen Y. G.
     Department of Prosthodontics, Sharavathi Dental College and Hospital, Shimoga 577205, Karnataka ,IN

Keywords:

Alginate, Pre-incorporated Quaternary, ammonium compound disinfectant, Sodium hypochlorite spray, disinfectant, Anticandidal activity, Denture stomatitis
Prosthodontics

Abstract

Context: A lot of research and literature exists regarding the methods of disinfecting alginates; however, there is still need for a infallible, quick, and standardized method, which can be used as chair side disinfectant for alginate impressions in patients with candida related denture stomatitis.
Aim: An In Vivo study was undertaken to evaluate the anticandidal activity of pre-incorporated quaternary ammonium compound disinfectant in the alginate with conventional alginate impression material disinfected with 5.25% sodium hypochlorite spray (1 min) in primary impressions of completely edentulous patients with denture stomatitis.
Methods and material: Group 1 consisted of 10 conventional alginate (Imprint) impressions disinfected by 5.25% sodium hypochlorite spray for 1 min. Group 2 consisted of 10 preincorporated quaternary ammonium compound alginates (Jeltrate Plus). Totally, 20 swabs (10 per group) were collected from palatal concavities of primary impressions and inoculated on Sabouraud GC Agar media at 37 °C for 24–96 h. Digital colony counter was used for counting colony-forming units (CFU).
Statistical analysis used: The data analysis was done using descriptive statistics and independent t-test.
Results: According to p value (p < 0.01), there was a statistical significant difference in mean values of the two groups. Group 1 showed significant anticandidal activity in comparison to Group 2.
Conclusions: According to limitations of this In Vivo study, sodium hypochlorite (5.25% for 1 min) spray disinfectant is more clinically effective as compared to the pre-incorporated quaternary ammonium compound alginate for patients with denture stomatitis. Sodium hypochlorite (5.25% for 1 min) spray disinfectant is a clinically effective disinfectant against Candida and saves clinical chairside time.

Downloads

Published

2018-09-18

How to Cite

Raval, H. J., Mahajan, N., Sethuraman, R., & Y. G., N. (2018). Comparative Evaluation of Anticandidal Activity of Pre-incorporated Quaternary Ammonium Compound Disinfectant Alginate With 5.25% Sodium Hypochlorite Spray Disinfectant on the Conventional Alginate: An In Vivo Study. Journal of Pierre Fauchard Academy (India Section), 31(2-4), 99–104. Retrieved from https://informaticsjournals.co.in/index.php/jpfa/article/view/21125

Issue

Volume 31, Issue 2-4, June - December 2017

Section

Original Articles

License

All the articles published in JPFA are distributed under a creative commons license. The journal allows the author(s) to hold the copyright of their work (all usages allowed except for commercial purpose).

 

References

Aas JA, Paster BJ, Stokes LN, Olsen I, Dewhirst FE. Defining the normal bacterial flora of the oral cavity. J Clin Microbiol. 2005;43:5721–5732.

Ribeiro DG, Pavarina AC, Dovigo LN, Machado AL, Giampaolo ET, Vergani CE. Prevalence of Candida spp. associated with bacteria species on complete dentures. Gerodontology. 2012;29:203–208.

Zegarelli DJ. Fungal infections of the oral cavity. Otolaryngol Clin North Am. 1993;26:1069–1089.

Zaremba ML, Daniluk T, Rozkiewicz D, et al. Incidence rate of Candida species in the oral cavity of middle-aged and elderly subjects. Adv Med Sci. 2006;51:233–236.

Lockhart SR, Joly S, Vargas K, Swails-Wenger J, Enger L, Soll DR. Natural defences against Candida colonization breakdown in the oral cavities of the elderly. J Dent Res. 1999;78:857–868.

Berdicevsky I, Ben-Aryeh H, Szargel R, Gutman D. Oral Candida of asymptomatic denture wearers. Int J Oral Surg. 1980;9:113–115.

Amalan A, Ginjupalli K, Upadhya N. Evaluation of properties of irreversible hydrocolloid impression materials mixed with disinfectant liquids. Dent Res J. 2013;10:65–73.

Flanagan DA, Palenik CJ, Setcos JC, Miller CH. Antimicrobial activities of dental impression materials. Dent Mater. 1998;14:399–404.

Doddamani S, Patil RA, Gangadhar SA. Efficacy of various spray disinfectants on irreversible hydrocolloid impression materials: an In Vitro study. Indian J Dent Res. 2011;22:764–769.

Cubas GBA, Valentini F, Camacho GB, Leite FRM, Cenci MS, Pereira-Cenci T. Antibacterial efficacy and effect of chlorhexidine mixed with irreversible hydrocolloid for dental impressions: a randomized controlled trial. Int J Prosthodont. 2014;27:363–365.

Samaranayake LP. Carriage of oral flora on irreversible hydrocolloid and elastomeric impression materials. J Prosthet Dent. 1991;65:244–249.

Hutchings ML, Vandewalle KS, Schwartz RS, Charlton DG. Immersion disinfection of irreversible hydrocolloid impressions in pH-adjusted sodium hypochlorite. Int J Prosthodont. 1996;9:223–229.

Infection control recommendations for the dental office and the dental laboratory. Council on Dental Materials, Instruments, and Equipment. Council on Dental Practice. Council on Dental Therapeutics. J Am Dent Assoc. 1988;116:241–248.

Taylor RL, Wright PS, Maryan C. Disinfection procedures: their effect on the dimensional accuracy and surface quality of irreversible hydrocolloid impression materials and gypsum casts. Dent Mater. 2002;18:103–110.

Jagger DC, Al Jabra O, Harrison A, Vowles RW, McNally L. The effect of a range of disinfectants on the dimensional accuracy of some impression materials. Eur J Prosthodont Restor Dent. 2004;12:154–160.

Hiraguchi H, Kaketani M, Hirose H, Yoneyama T. The influence of storing alginate impressions sprayed with disinfectant on dimensional accuracy and deformation of maxillary edentulous stone models. Dent Mater J. 2010;29:309–315.