Remineralization of Incipient Lesions with Anticariogenic Casein Phosphopeptides and Fluoride: A Vivo Study

Jump To References Section

Authors

  • Ritu Khanduja
     Department of Pedodontics and Preventive Dentistry, K.D. Dental College and Hospital, Mathura, Kota – 281121, Uttar Pradesh ,IN
  • Rupanjali Verma
     Post Graduate Student, Department of Pedodontics and Preventive Dentistry, K.D. Dental College and Hospital, Mathura, Kota – 281121, Uttar Pradesh ,IN
  • Shobha Tandon
     Department of Pedodontics and Preventive Dentistry, Manipal College of Dental Sciences, Manipal – 576104, Karnataka ,IN

DOI:

https://doi.org/10.18311/jpfa/2019/23832

Keywords:

CPP-ACP, Fluoride, Remineralization
Pedodontics

Abstract

Objective: The aim of this study was to evaluate the remineralization of incipient lesions with CPP-ACP and its comparison with the effect of fluoride on the same. Study Design: A total of 225 teeth with incipient lesions in 108 children within the age group of 6-14 yrs. were divided into five groups by block randomization. Enamel biopsy was done for incipient lesions. Calcium and phosphate were analyzed using auto analyzer, fluoride was assessed using Orion analyzer. Results: Results showed that CPP-ACP enhances the remineralization process and that CPP-ACP has comparable effect as fluoridated dentifrice, as difference was not statistically significant (p>0.05), using ANOVA, Tukey's test. Conclusion: CPP-ACP is effective remineralizing agent. A beneficial dose response effect is seen with the application of casein phosphopeptides. In addition, there is a synergistic effect of CPP-ACP with fluoride.

Downloads

Published

2019-11-29

How to Cite

Khanduja, R., Verma, R., & Tandon, S. (2019). Remineralization of Incipient Lesions with Anticariogenic Casein Phosphopeptides and Fluoride: A Vivo Study. Journal of Pierre Fauchard Academy (India Section), 33(4), 102–109. https://doi.org/10.18311/jpfa/2019/23832

Issue

Volume 33, Issue 4, December 2019

Section

Original Articles

License

All the articles published in JPFA are distributed under a creative commons license. The journal allows the author(s) to hold the copyright of their work (all usages allowed except for commercial purpose).

 

References

Head J. Enamel softening and rehardening as a factor in erosion. Dental Cos. 1909; 6:46.

Silverstone L.M. Caries and Remineralization. Dent. Hyg. 1983; 57(5):30–36.

Reynolds EC, inventor. The University of Melbourne and the Victorian Dairy Industry Authority, assignees. Anticariogenic phosphopeptides; 1991 May 14.

Kariya, Sato T, Yamanaka K, Yoshii E. Fluoride effect on acid resistance capacity of CPP-ACP containing material. Pub med abstract no: 2045; 2004.

Vogel, C., Brown. Micro-analytical procedure for the determination of calcium, phosphate and fluoride in enamel biopsy samples. Caries Res. 1983; 17:23–31. https://doi.org/10.1159/000260645. PMid:6571804

Frant and Ross. Use of total ionic strength adjustment buffer for electrode determination of fluoride in water. Anal Chem. 1968; 40:1169. https://doi.org/10.1021/ac60263a005. PMid:5647897

Roberts AJ. Role of models in assessing new agents for caries prevention - non-fluoride systems. Adv Dent Res. 1995. 9:304–11. https://doi.org/10.1177/089593749500900 31601. PMid:8615950

Silness J, Loe H. Periodontal disease in children, correlation between oral hygiene and periodontal condition. Acta Odont Scan. 1964, 22:121–135. https://doi.org/10.3109/00016356408993968. PMid:14158464

Loe H, Silness J. Periodontal disease in pregnancy I: Prevalence and severity. Acta Odont Scan. 1967; 21:533– 551. https://doi.org/10.3109/00016356309011240. PMid:14121956

Creanor SL, Hall AF, Gilmour, Strang, Foye. Nonfluoridated dentifrice and enamel lesion - remineralization in situ. Caries res. (abstract): 41st ORCA congress; 1994.

Joyston K. Remineralization of carious lesions in enamel by exposure to fluoride containing toothpaste in vitro. Brt. Dent. J. 1986; 61:133–137. https://doi.org/10.1038/sj.bdj.4805910. PMid:3461837

Ashirov KA. Changes in the properties of the enamel of the permanent teeth in children using fluoride-containing toothpastes. Stomatologiia (Mosk).1994; 73(4):51–54.

Shen P, Cai F, Nowicki A, Vincent J, Reynolds EC. Remineralization of enamel subsurface lesions by sugar-free chewing gum containing casein phosphopeptide-amorphous calcium phosphate. J Dent Res. 2001; 80(12):2066–2070. https://doi.org/10.1177/00220345010800120801. PMid:11808763

Iijima Y, Cai F, Shen P, Walker G, Reynolds C, Reynolds EC. Acid resistance of enamel subsurface lesions remineralized by a sugar-free chewing gum containing casein phosphopeptide-amorphous calcium phosphate. Caries Res. 2004; 38(6):551–556. https://doi.org/10.1159/000080585. PMid:15528910

Black FC, Cross KJ, Eakins D, Huq NL, Morgan MV, Perich JW, Riley PF, Shen P, Talbo G, Webber F. Advances in enamel remineralization: Casein Phosphopeptide ¬ Amorphous Calcium Phosphate. J Clin Dent. 1999; 10:86–88.

Schemehorn BR, Orban JC, Wood GD, Fischer GM, Winston AE. Remineralization by fluoride enhanced with calcium and phosphate ingredients. J Clin Dent. 1999; 10(1 Spec No):13–6.

Tanaka, KY. Comparative reduction of enamel demineralization of calcium and phosphate in vitro. Caries Res. 2000; 34:241–245. https://doi.org/10.1159/000016597. PMid:10867423

Hay KD, Morton RP. Casein derivatives coupled with calcium phosphate as a mouth moistener in patients with severe xerostomia. New Zealand Dental Journal. 2003; 34:301–305.

Varughese K, Moreno EC. Crystal growth of calcium apatites in dilute solutions containing fluoride. Calcif Tissue Int. 1981, 33:431–439. https://doi.org/10.1007/BF02409467. PMid:6794881

Koulourides T. Implication of remineralization in the treatment of dental caries. In proceedings symposium on current topics in dental caries in commemoration of the decennial anniversary of Hihon university school of dentistry at Mastsudo, Japan; 1982.

Racquel Z, LeGeros. Calcium phosphates in demineralization/ remineralization processes. J Clin Dent.

;10:65–73.

Zhao Q, Cai F. Remineralizing effect of Casein phosphopeptide-amorphous calcium fluoride phosphate complexes (CPP-ACFP). Chin J Dent Res. 2004; (3–4):69–73.

Rose, Turner SJ. Calcium mobility in streptococcal model plaques: Effect of fluoride. Caries Res. (abstracts); 1996; 30:267–312.

Rolla G, Bjorn O. Clinical effect and mechanism of cariostatic action of fluoride-containing toothpastes: A review. Int. Dent. J. 1991; 41:171–174.

Rose RK. Binding characteristics of Streptococcus mutans for calcium and casein phosphopeptide. Caries Res. 2000; 34(5):427–431. https://doi.org/10.1159/000016618. PMid:11014910

Hamilton.IR. Biochemical effects of fluorides on oral bacteria. J Dent Res. 1990; 69:660. https://doi.org/10.1177/00220345900690S128. PMid:2179327