Comparative Study on Phytochemical Constituents, Antimicrobial and Plasmid Curing Property of <I>Aloe vera</I> and <I>Ocimum sanctum</I>

Arpita Tripathi; S. A. John; K. P. Rao; P. W. Ramteke

doi:10.18311/jeoh/2013/1712

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Authors

Arpita Tripathi
Department of Biological Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences (Formerly Allahabad Agricultural Institute), Allahabad ,IN
S. A. John
Department of Biological Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences (Formerly Allahabad Agricultural Institute), Allahabad ,IN
K. P. Rao
Department of Biological Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences (Formerly Allahabad Agricultural Institute), Allahabad ,IN
P. W. Ramteke
Department of Biological Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences (Formerly Allahabad Agricultural Institute), Allahabad ,IN

DOI:

https://doi.org/10.18311/jeoh/2013/1712

Keywords:

Phytochemicals, Plasmid Curing, Reflux Extraction, Aloe vera, Ocimum sanctum, Agar well Diffusion, Escherichia coli and Bacillus cereus.

Abstract

This study investigated phytochemical content, antimicrobial and plasmid curing property of four extracts (viz. petroleum ether, ethanol, distilled water and acetone) by reflux extraction technique of leaves of Aloe vera and Ocimum sanctum. Qualitative and quantitative analysis of secondary metabolites were carried out. Agar well diffusion method was used for antibacterial activity against Escherichia coli and Bacillus cereus and plasmid curing property of phytoextracts was carried out. The result revealed that the tested plant materials contained appreciable amounts of total alkaloids (16.00- 71.00 mg/gm leaves), total phenols (10.233-40.267 Î¼g g^-1 fw), total saponins (10.267-65.167 Î¼g g^-1 fw) zone of inhibition (0.00-12.33 mm) and plasmid curing property (81-84%). Generally higher extract yields, secondary metabolites, antimicrobial activity and plasmid curing property were obtained using ethanol as a solvents, as compared to the respective other solvents.