Apoptotic Response of Human Mesenchymal Stem Cells against Dose Dependent Exposure to Organophosphate Pesticide-Monocrotophos
Authors
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System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), MG Marg, Lucknow – 226001, Uttar Pradesh ,IN
Akriti Srivastava -
System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), MG Marg, Lucknow – 226001, Uttar Pradesh ,INPankhi Vatsa
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System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), MG Marg, Lucknow – 226001, Uttar Pradesh ,INShripriya Singh
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System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), MG Marg, Lucknow – 226001, Uttar Pradesh ,INDipak Kumar
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System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), MG Marg, Lucknow – 226001, Uttar Pradesh ,INAditya Bhushan Pant
DOI:
https://doi.org/10.18311/jeoh/2017/17922Keywords:
Apoptosis, Cytotoxicity, Monocrotophos, Stem Cells.Abstract
The severe health implications associated with the exposure of organophosphate pesticides are highly pronounced owing to their indiscriminate use. Monocrotophos, a predominantly used organophosphate pesticide, is a hazardous chemical that predisposes the exposed population towards multiple health disorders. We investigated the toxic effects of MCP on human mesenchymal stem cells to ascertain its overall detrimental effects on the normal physiology. There was a remarkable reduction in the cell viability accompanied with increased oxidative stress and mitochondrial dysfunctions with the increasing exposure concentrations. The decreased cell viability is probably an outcome of monocrotophos induced apoptotic cell death indicated by elevated expression of Bax and caspase-3 and reduced BCl2 expression and as such calls for a stringent abatement in its haphazard use to prevent the incidence of many life threatening ailments.
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Ali, S.J. and Rajini, P.S. (2016) "Effect of monocrotophos, an organophosphorus insecticide, on the striatal dopaminergic system in a mouse model of Parkinson's disease", Toxicology and Industrial Health, 32, 1153–1165. Crossref PMid:25227224
Armario, A., Daviu, N., Mu-oz-Abellán, C., Rabasa, C., Fuentes, S., Belda, X., Gagliano, H. and Nadal, R. (2012) "What can we know from pituitary–adrenal hormones about the nature and consequences of exposure to emotional stressors"? Cellular and Molecular Neurobiology, 32, 749–758. Crossref PMid:22392360
Campbell, J. and Ehlert, U. (2012) "Acute psychosocial stress: Does the emotional stress response correspond with physiological responses"? Psychoneuroendocrinology, 37, 1111–1134. Crossref PMid:22260938
Cannon, J.R. and Greenamyre, J.T. (2011) "The role of environmental exposures in neurodegeneration and neuro degenerative diseases", Toxicological Sciences, 124, 225–50. Crossref PMid:21914720 PMCid:PMC3216414
Chambers, J.E. and Oppenheimer, S.F. (2004) "Organophosphates, serine esterase inhibition, and modeling of organophosphate toxicity", Toxicological Sciences, 77, 185–187. Crossref PMid:14992203
Costa, L.G. (2008) "Toxic effects of pesticides", Casarett and Doull's Toxicology: The Basic Science of Poisons, 8, 883–930.
Costa, L.G., Cole, T.B., Vitalone, A. and Furlong, C.E. (2005) "Measurement of paraoxonase (PON1) status as a potential biomarker of susceptibility to organophosphate toxicity", Clinica Chimica Acta, 352, 37–47. Crossref PMid:15653099
Dardiotis, E., Xiromerisiou, G., Hadjichristodoulou, C., Tsatsakis, A.M., Wilks, M.F. and Hadjigeorgiou, G.M. (2013) "The interplay between environmental and genetic factors in Parkinson's disease susceptibility: The evidence for pesticides", Toxicology, 307, 17–23. Crossref PMid:23295711
Garcia, F.P., Ascencio, S.Y.C., Oyarzun, J.C.G., Hernandez, A.C and Alavarado, P.V. (2012) "Pesticides: Classification, uses and toxicity. Measures of exposure and genotoxic risks", Journal of Research in Environmental Science and Toxicology, 1, 279–293.
Gomez-Mejiba, S.E., Zhai, Z., Akram, H., Pye, Q.N., Hensley, K., Kurien, B.T., Scofield, R.H. and Ramirez, D.C. (2009) "Inhalation of environmental stressors and chronic inflammation: autoimmunity and neurodegeneration", Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 674, 62–72. Crossref PMid:18977456 PMCid:PMC2676865
Hayden, K.M., Plassman, B.L., Potter, G.G., Norton, M.C., Tschanz, J.T., Darcey, D.J., Zandi, P.P., Breitner, J.C., WelshBohmer, K.A. (2014) "The association between lifetime occupational exposure to pesticides and cognitive decline in the elderly", Alzheimer's & Dementia: The Journal of the Alzheimer's Association, 10, P266–P267. Crossref
Ibrahim, Y.A. (2016) "Health and environmental impacts of pesticides: A responsibility principle and two novel systems for hazard classification and external cost determination", Journal of Toxicology and Health, 3, 1. Crossref
Jokanović, M. and Kosanović, M. (2010) "Neurotoxic effects in patients poisoned with organophosphorus pesticides", Environmental Toxicology and Pharmacology, 29, 195–201. Crossref PMid:21787602
Karami-Mohajeri, S. and Abdollahi, M. (2013) "Mitochondrial dysfunction and organophosphorus compounds", Toxicology and Applied Pharmacology, 270, 39–44. Crossref PMid:23578477
Karthikeyan, P., Mohan, D. and Jaikumar, M. (2015) "Growth Inhibition Effect of Organophosphate Pesticide, Monocrotophos on Marine Diatoms", Indian Journal of Geo-Marine Sciences, 44, 1516–1520.
Kashyap, M., Singh, A., Kumar, V., Tripathi, V., Srivastava, R., Agrawal, M., Khanna, V.K., Yadav, S., Jain, S.K. and Pant, A.B. (2011) "Monocrotophos Induced Apoptosis in PC12 Cells: Role of Xenobiotic Metabolizing Cytochrome P450s", PLoS ONE, 6, e17757. Crossref PMid:21445290 PMCid:PMC3061860
Kashyap, M., Singh, A., Kumar, V., Yadav, D.K., Khan, F., Jahan, S., Khanna, V.K., Yadav, S. and Pant, A.B. (2013) "Pkb/Akt1 Mediates Wnt/GSK3b/b-catenin signalinginduced apoptosis in human cord blood stem cells exposed to organophosphate pesticide monocrotophos", Stem Cells and Development, 22, 224–238. Crossref PMid:22897592 PMCid:PMC3545351
Kashyap, M., Singh, A., Siddiqui, M., Kumar, V., Tripathi, V., Khanna, V., Yadav, S., Jain, S. and Pant, A. (2010) "Caspase cascade regulated mitochondria mediated apoptosis in monocrotophos exposed PC12 cells", Chemical Research in Toxicology. 23, 1663–1672. Crossref PMid:20957986
Krause, K.-H., van Thriel, C., De Sousa, P.A., Leist, M. and Hengstler, J.G. (2013) "Monocrotophos in Gandaman village: India school lunch deaths and need for improved toxicity testing", Archives of toxicology, 87, 1877–1881. Crossref PMid:23943209
Kumar, V., Tripathi, V.K., Singh, A.K., Lohani, M. and Kuddus, M. (2013) "Trans-resveratrol restores the damages induced by organophosphate pesticide-monocrotophos in neuronal cells", Toxicology International, 20, 48–55. Crossref PMid:23833438 PMCid:PMC3702127
Morris, C.M., Savy, C., Judge, S.J. and Blain, P.G. (2014) Acute Toxicity of Organophosphorus Compounds. Basic and Clinical Toxicology of Organophosphorus Compounds, Springer, 45–78. Crossref
Mosmann, T. (1983) "Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays", Journal of Immunological Methods, 65, 55–63. Crossref
Mostafalou, S. and Abdollahi, M. (2013) "Pesticides and human chronic diseases: evidences, mechanisms, and perspectives", Toxicology and Applied Pharmacology, 268, 157–177. Crossref PMid:23402800
Ojha, A., Yaduvanshi, S.K., Pant, S.C., Lomash, V. and Srivastava, N. (2013) "Evaluation of DNA damage and cytotoxicity induced by three commonly used organophosphate pesticides individually and in mixture, in rat tissues", Environmental Toxicology, 28, 543–552. Crossref
Repetto, G., Del Peso, A. and Zurita, J.L. (2008) "Neutral red uptake assay for the estimation of cell viability/cytotoxicity", Nature Protocols, 3, 1125–1131. Crossref PMid:18600217
Schulte, P.M. (2014) "What is environmental stress? Insights from fish living in a variable environment", Journal of Experimental Biology, 217, 23–34. Crossref PMid:24353201
Strober, W. (2001). "Trypan blue exclusion test of cell viability", Current Protocols in Immunology, 21, 3B:A.3B.1– A.3B.2.
Tripathi, V.K., Kumar, V., Singh, A.K., Kashyap, M.P., Jahan, S., Pandey, A., Alam, S., Khan, F., Khanna, V.K., Yadav, S., Lohani, M. and Pant, A.B. (2014) "Monocrotophos induces the expression and activity of xenobiotic metabolizing enzymes in pre-sensitized cultured human brain cells", PLoS ONE, 9, e91946. Crossref PMid:24663500 PMCid:PMC3963866
Velmurugan, G., Babu, D.V. and Ramasamy, S. (2013) "Prolonged monocrotophos intake induces cardiac oxidative stress and myocardial damage in rats", Toxicology, 307, 103–108. Crossref PMid:23228476
Venkatesh, S., Ramachandran, A., Zachariah, A. and Oommen, A. (2009) "Mitochondrial ATP synthase inhibition and nitric oxide are involved in muscle weakness that occurs in acute exposure of rats to monocrotophos", Toxicology Mechanisms and Methods, 19, 239–245. Crossref PMid:19730754 PMCid:PMC2736536
Violi, F., Filippini, T., Malagoli, C., Mandrioli, J., Signorelli, C., Odone, A., Ferrante, M., Fiore, M., Ledda, C. and Mauceri, C. (2015) "Risk of ALS and passive long-term residential exposure to pesticides: A population based study", The European Journal of Public Health, 25, ckv175. 053. Crossref
Voorhees, J.R., Rohlman, D.S., Lein, P.J. and Pieper, A.A. (2016) "Neurotoxicity in preclinical models of occupational exposure to organophosphorus compounds", Frontiers in Neuroscience, 10, 590. PMid:28149268
Yaduvanshi, S.K., Ojha, A., Pant, S.C., Lomash, V. and Srivastava, N. (2010) "Monocrotophos induced lipid peroxidation and oxidative DNA damage in rat tissues", Pesticide Biochemistry and Physiology, 97, 214–222. Crossref
