Biochemical constituents, protein profile and effect of male accessory gland extract on egg production in mother moth of Antheraea mylitta Drury

Abstract

Male accessory gland factors greatly influence the ovulation and oviposition and male receptivity behaviour of female insects. Some important biochemical constituents available in the male accessory gland extract of Antheraea mylitta during first and second crop grainage were analyzed and injected to just decouple mother tasar moth to observe the impact of extract injection on egg laying parameters. During first crop grainage, in just emerged male moth accessory gland extract the concentration of amino acid was recorded to be 0.049 ± 0.095 to 0.033 ± 0.012 mg/ml, glycerol - 0.007 ± 0.001 to 0.019 ± 0.004 milimole/ml, glycogen 0.827± 0.164 to 1.219 ± 0.060 mg/ml, protein - 9.405 ± 0.417 to 19.305 ± 0.946 to mg/ml, trehalose - 0.080 ± 0.059 to 0.295 ± 0.028 mg/ml and lipid 0.151 ± 0.008 to 0.263±Q.001g/ml. During second crop concentration of amino acid was recorded to be 0.009 ± 0.001 to 0.057±0.002 mg/ml, glycerol - 0.005 ± 0.001 to 0.016±0.0001 milimole/ml, glycogen 0.997± 0.700 to 1.482±1.217 mg/ml, protein- 3.389 ± 1.841 to 9.141 ± 3.023 to mg/ml, trehalose - 0.085 ± 0.029 to 0.132 ± 0.036 mg/ml and lipid 0.050 ± 0.012 to 0.510 ± 0.071g/ ml. The concentration of total proteins and lipid was also recorded to be in higher proportion than other biochemical constituents, extract of lOpI of single male accessory gland in first crop and 20pl in second crop grainage enhanced fecundity at 26' C. No difference in the qualitative protein profile of male accessory gland extract of0to6h old males was observed. There were altogether 17 detectable protein bands ranging in between molecular weight of 8.403 to 155.595 kD. When male moths were 28 hr old a higher molecular weight protein band of 184.746kD appeared. Very low molecular protein band of8.403kD disappeared. When male moths became older i.e of 24 to 36 hours old total 18 bands were seen in the molecular weight range of 155.95 to 8.779kD