Effect of Artemisia vulgaris Isolated Extract and Determination of AD50 and LD50 for Antifertility and Mortality of Callosobruchus chinensis (L.)

Abstract

Antifertility, AD₅₀ with effect of Artemisia vulgaris isolated leaf extract on hatchability was noted. These median antifertility doses 0.6529μg, 0.4753 percent and 0.5198 percent per weevil were obtained in topical, dipping and injection (12μl/weevil) methods respectively. Probit analysis for dose response data for Artemisia vulgaris leaf extract showed that linear relationship existed in between the concentration (Log x) and percentage antifertility induced (Probit Y). Mortality, LD₅₀ was observed with effect of Artemisia vulgaris, isolated leaf extract during hatchability were 0.7589ï­g; 0.4743 percent and 0.5139 percent per weevil were obtained in topical, dipping and injection (12μl/weevil) methods respectively. Probit analysis showed that a linear relationship existed in between the concentration (Log x) and percent mortality induced (Probit Y).