Biogenic Synthesis and characterization of Zinc oxide Nanoparticles by Using Green Machinery: Antibacterial and Antibiofilm potential

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Authors

  • Prakriti Mishra
     Department of Biosciences, Integral University, Lucknow – 226026, Uttar Pradesh ,IN ORCID logo http://orcid.org/0000-0001-5641-4710
  • Naushin Bano
     Department of Biosciences, Integral University, Lucknow – 226026, Uttar Pradesh ,IN
  • Irfan Ahmad Ansari
     Department of Biosciences, Integral University, Lucknow – 226026, Uttar Pradesh ,IN

DOI:

https://doi.org/10.18311/jeoh/2022/29338

Keywords:

Antibacterial, Antibiofilm, Cannabis sativa, Green Synthesis, Zinc Oxide Nanoparticles (ZnONPs)

Abstract

In the field of biomedicine, the green synthesis of Zinc Oxide Nanoparticles (ZnONPs) utilising plant extracts has piqued interest. The reduction nature of herbal extracts has recently aided in the production of spherical ZnONPs of various potentials from zinc salt. In this study, fresh leaf (aqueous) extracts of Cannabis sativa were used as reducing and stabilising agents in a rapid, environmentally friendly approach for the synthesis of ZnONPs. UV–VIS and Fourier transform infrared spectroscopy, as well as transmission electron microscopy, were used to analyse the biosynthesized CNS-ZnONPs (TEM). The antibacterial and antibiofilm properties of produced CNS-ZnONPs were also studied in vitro. The presence of a prominent absorption peak at 380 nm, which corresponds to the CNSZnONPs’ Surface Plasmon Resonance (SPR) band, indicated the creation of CNS-ZnONPs. The produced CNS-ZnONPs were spherical in shape, with an average particle size of 16.25 nm, according to TEM examination. The synthesised CNS-ZnONPs also showed significant antibacterial activity against a variety of Gram-positive and Gram-negative microorganisms. Furthermore, the biosynthesized CNSZnONPs significantly reduced biofilm formation. Cannabis sativa leaf extracts may be utilised to easily synthesise ZnONPs, which can be employed as a natural source of antibacterial and antibiofilm agents.

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Published

2022-04-05

How to Cite

Mishra, P., Bano, N., & Ansari, I. A. (2022). Biogenic Synthesis and characterization of Zinc oxide Nanoparticles by Using Green Machinery: Antibacterial and Antibiofilm potential. Journal of Ecophysiology and Occupational Health, 22(1), 1–7. https://doi.org/10.18311/jeoh/2022/29338

Issue

Volume 22, Issue 1, March 2022

Section

Articles
Crossref
Scopus
Google Scholar
Europe PMC
Received 2022-01-17
Accepted 2022-01-24
Published 2022-04-05

 

References

Ding Q, Kang Z, Cao L, Lin M, Lin H, Yang DP. Conversion of waste eggshell into difunctional Au/CaCO3 nanocomposite for 4-Nitrophenol electrochemical detection and catalytic reduction. Appl Surf Sci. 2020; 510:145526. https://doi.org/10.1016/j.apsusc.2020.145526

Huang J, Lin L, Sun D, Chen H, Yang D, Li Q. Bio-inspired synthesis of metal nanomaterials and applications. Chem Soc Rev. 2015; 44:6330-6374. https://doi.org/10.1039/C5CS00133A PMid:26083903

Ayaz M, Ovais M, Ahmad I, Sadiq A, Khalil AT, Ullah F. Biosynthesized metal nanoparticles as potential Alzheimer’s disease therapeutics. In Metal Nanoparticles for Drug Delivery and Diagnostic. Elsevier; 2020; pp. 31-42. https://doi.org/10.1016/B978-0-12-816960-5.00003-3

Mukherjee S, Biosynthesized silver nanoparticles in cancer theranostics applications. Biogenic Nanoparticles for Cancer Theranostics. Elsevier; 2021. p. 141-152. https://doi.org/10.1016/B978-0-12-821467-1.00002-1 PMid:33126048

Khalil AT, Ovais M, Iqbal J, Ali A, Ayaz M, Abbas M, Devkota HP. Microbes-mediated synthesis strategies of metal nanoparticles and their potential role in cancer therapeutics. Seminars in Cancer Biology. Elsevier; 2021. https://doi.org/10.1016/j.semcancer.2021.06.006 PMid:34118405

Ovais M, Khalil AT, Ayaz M, Ahmad I, Nethi SK, Mukherjee S. Biosynthesis of metal nanoparticles via microbial enzymes: A mechanistic approach. Int J Mol Sci. 2018; 19:4100. https://doi.org/10.3390/ijms19124100 PMid:30567324 PMCid: PMC6321641

Baker A, Iram S. Syed A, Elgorban AM, Bahkali AH, Ahmad K, Khan MS. Kim J. Fruit derived potentially bioactive bioengineered silver nanoparticles. Int J Nanomedicine. 2021; 16:7711. https://doi.org/10.2147/IJN.S330763 PMid:34848956 PMCid:PMC8612025

Haider A, Ijaz M, Imran M, Naz M, Majeed H, Khan JA, Ali MM, Ikram M. Enhanced bactericidal action and dye degradation of spicy roots’ extract-incorporated fine-tuned metal oxide nanoparticles. Appl Nanosci. 2020; 10:1095-1104. https://doi.org/10.1007/s13204-019-01188-x

Manna J, Begum G, Kumar KP, Misra S, Rana RK. Enabling antibacterial coating via bioinspired mineralization of nanostructured ZnO on fabrics under mild conditions. ACS Appl Mater Interfaces. 2013; 5:4457-4463. https://doi.org/10.1021/am400933n PMid:23607588

Al-Hada NM, Al-Ghaili AM, Kasim H, Saleh MA, Baqiah H, Liu J, Wang J. Preparation of hybrid nanoparticles to enhance the electrical conductivity and performance properties of cotton fabrics. J Mater Res Technol. 2021; 12:542-554. https://doi.org/10.1016/j.jmrt.2021.02.035

Shaheen TI, El-Naggar ME, Abdelgawad AM, Hebeish. Durable antibacterial and UV protections of in situ synthesized zinc oxide nanoparticles onto cotton fabrics. Int J Biol Macromol. 2016; 83:426-432. https://doi.org/10.1016/j.ijbiomac.2015.11.003 PMid:26546870

Selvakesavan RK, Franklin G. Prospective application of nanoparticles green synthesized using medicinal plant extracts as novel nanomedicines. Nanotechnol Sci Appl. 2021; 14:179. https://doi.org/10.2147/NSA.S333467 PMid:34588770 PMCid:PMC8476107

Abdo AM, Fouda A, Eid AM, Fahmy NM, Elsayed AM, Khalil AMA, Alzahrani OM, Ahmed AF, Soliman AM. Green synthesis of Zinc Oxide Nanoparticles (ZnO-NPs) by pseudomonas aeruginosa and their activity against pathogenic microbes and common house mosquito, culex pipiens. Materials 2021; 14:6983. https://doi.org/10.3390/ma14226983 PMid:34832382 PMCid:PMC8623893

Pachaiappan R, Rajendran S, Ramalingam G, Vo DVN, Priya PM, Soto-Moscoso M. Green synthesis of zinc oxide nanoparticles by justicia adhatoda leaves and their antimicrobial activity. Chem Eng Technol. 2021; 44:551-558. https://doi.org/10.1002/ceat.202000470

Hatami Z, Ragheb E, Jalali F, Tabrizi MA, Shamsipur M. Zinc oxide-gold nanocomposite as a proper platform for label-free DNA biosensor. Bioelectrochemistry 2020; 133, 107458. https://doi.org/10.1016/j.bioelechem.2020.107458 PMid:32006859

Ramirez-Vick JE. Nanostructured ZnO for electrochemical biosensors. J Biosens Bioelectron. 2012; 3. https://doi.org/10.4172/2155-6210.1000e109

Lakhan MN, Chen R, Shar AH, Chand K, Shah AH, Ahmed M, Ali I, Ahmed R, Liu J, Takahashi K, Wang J. Eco-friendly green synthesis of clove buds extracts functionalized silver nanoparticles and evaluation of antibacterial and antidiatom activity. J Microbiol Methods. 2020; 173:105934. https://doi.org/10.1016/j.

mimet.2020.105934 PMid:32325159

Mosnier JP, O’Haire RJ, McGlynn E, Henry MO, McDonnell SJ, Boyle MA, McGuigan KG. ZnO films grown by pulsed-laser deposition on soda lime glass substrates for the ultraviolet inactivation of Staphylococcus epidermidis biofilms. Sci Technol Adv Mater. 2009. https://doi.org/10.1088/1468-6996/10/4/045003 PMid:27877303 PMCid:PMC5090265

Xu S, Sun T, Xu Q, Duan C, Dai Y, Wang L, Song Q. Preparation and antibiofilm properties of zinc oxide/porous anodic alumina composite films. Nanoscale Res Lett. 2018; 13:1-12. https://doi.org/10.1088/1468-6996/10/4/045003 PMid:27877303 PMCid:PMC5090265

Singh A, Bilichak A, Kovalchuk I. The genetics of Cannabisgenomic variations of key synthases and their effect on cannabinoid content. Genome. 2021; 64:490-501. https://doi.org/10.1139/gen-2020-0087 PMid:33186070

Jiang HE, Li X, Zhao YX, Ferguson DK, Hueber F, Bera S, Wang YF, Zhao LC, Liu CJ, Li CS. A new insight into Cannabis sativa (Cannabaceae) utilization from 2500-year-old Yanghai Tombs, Xinjiang, China. J Ethnopharmacol. 2006; 108:414-422. https://doi.org/10.1016/j.jep.2006.05.034 PMid:16879937

Khan MA, Khan T, Nadhman A. Applications of plant terpenoids in the synthesis of colloidal silver nanoparticles. Adv Colloid Interface Sci. 2016; 234:132-141. https://doi.org/10.1016/j.cis.2016.04.008 PMid:27181393

Bisogno T, Lauritano A, Piscitelli F. The endocannabinoid system: A bridge between alzheimer’s disease and gut microbiota. Life. 2021; 11:934. https://doi.org/10.3390/life11090934 PMid:34575083 PMCid:PMC8470731

Cristino L, Bisogno T, Di Marzo V. Cannabinoids and the expanded endocannabinoid system in neurological disorders. Nat Rev Neurol. 2020; 16:9-29. https://doi.org/10.1038/s41582019-0284-z PMid:31831863

Messaoudi O, Bendahou M, Benamar I, Abdelwouhid DE, Abdelwouhid. Identification and preliminary characterization non-polyene antibiotics secreted by new strain of actinomycete isolated from sebkha of Kenadsa, Algeria. Asian Pac. J Trop Biomed. 2015; 5:438-445. https://doi.org/10.1016/j.apjtb.2015.04.002

Baker A, Iram S, Syed A, Elgorban AM, Al-Falih AM, Bahkali AH, Khan MS, Kim J. Potentially bioactive fungus mediated silver nanoparticles. Nanomaterials. 2021; 11:1-20. https://doi.org/10.3390/nano11123227 PMid:34947576 PMCid:PMC8706101

Wypij M, Czarnecka J, ?wiecimska M, Dahm H, Rai M, Golinska P. Synthesis, characterization and evaluation of antimicrobial and cytotoxic activities of biogenic silver nanoparticles synthesized from Streptomyces xinghaiensis OF1 strain. World J Microbiol Biotechnol. 2018; 34:1-13. https://doi.org/10.1007/s11274-0172406-3 PMid:29305718 PMCid:PMC5756267

Contreras-Lynch S, Smith P, Olmos P, Loy ME, Finnegan W, Miranda CD. A novel and validated protocol for performing MIC tests to determine the susceptibility of Piscirickettsia salmonis isolates to florfenicol and oxytetracycline. Front Microbiol. 2017; 8:1-7. https://doi.org/10.3389/fmicb.2017.01255 PMid:28729865 PMCid:PMC5498515

Xie YY, Zhang YW, Liu XZ, Ma XF, Qin XT, Jia SR, Zhong C. Aggregation-induced emission-active amino acid/berberine hydrogels with enhanced photodynamic antibacterial and antibiofilm activity. Chem Eng J. 2021; 413:127542. https://doi.org/10.1016/j.cej.2020.127542

Fatima A, Kazmi MB, Yasmeen H, Yasmeen. Biofilm forming bacteria isolated from medical implants. Adv Life Sci. 2021; 8:251-256.

da Silva RA, Afonina I, Kline KA. Eradicating biofilm infections: an update on current and prospective approaches. Curr Opin Microbiol. 2021; 63:117-125. https://doi.org/10.1016/j.mib.2021.07.001 PMid:34333239

Muhsin J, Wisal A, Saadia A, Fazal J, Muhammad I, Muhammad AN, Tahir H, Muhammad A, Muhammad R, Muhammad AK. Bacterial biofilm and associated infections. Journal of the Chinese

Medical Association. 2018; 81(1):7-11. https://doi.org/10.1016/j.jcma.2017.07.012 PMid:29042186

Pusparajah P, Letchumanan V, Law JWF, Ab Mutalib NS, Ong YS, Goh BH, Tan LTH, Lee LH. Streptomyces sp. A treasure trove of weapons to combat methicillin-resistant Staphylococcus aureus

biofilm associated with biomedical devices. Int J Mol Sci 2021; 22:9360, https://doi.org/10.3390/ijms22179360 PMid:34502269 PMCid:PMC8431294

Mangzira Kemung H, Tan LTH, Chan KG, Ser HL, Law JWF, Lee LH, Goh BH. Streptomyces sp. Strain MUSC 125 from Mangrove Soil in Malaysia with Anti-MRSA. Anti-Biofilm and Antioxidant Activities. Molecules. 2020; 25:3545. https://doi.org/10.3390/molecules25153545 PMid:32756432 PMCid:PMC7435833

Bano N, Siddiqui S, Amir M, Zia Q, Banawas S, Iqbal D, Roohi. Bioprospecting of the novel isolate Microbacterium proteolyticum LA2(R) from the rhizosphere of Rauwolfia serpentine. Saudi J Biol Sci. 2021; 2. https://doi.org/10.1016/j.sjbs.2021.10.038 PMid:34588855 PMCid:PMC8459126

Alvi SS, Ansari IA, Ahmad MK, Iqbal J, Khan MS. Lycopene amends LPS induced oxidative stress and hypertriglyceridemia via modulating PCSK-9 expression and Apo-CIII mediated lipoprotein lipase activity. Biomed Pharmacother. 2017; 96:1082-1093. https://doi.org/10.1016/j.biopha.2017.11.116 PMid:29174038

Bano N, Siddiqui S, Amir M, Roohi. House of industrially important bioactive metabolites: A review on actinobacteria. Indian J Biotechnol. 2019; 18:293-304.