Evaluating Rice for Salinity and Submergence Using Pot-Culture Provides a Systematic Tolerance Assessment at the Seedling Stage

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Authors

  • R. Muthu Vijayaragavan
     Department of Plant Breeding and Genetics, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai - 625 104 ,IN
  • E. Murugan
     Department of Plant Breeding and Genetics, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai - 625 104 ,IN

DOI:

https://doi.org/10.21048/IJND.2023.60.1.29731

Keywords:

Rice (Oryza sativa L.), Physiological traits, Pot-culture screening, Salinity tolerance, Submergence tolerance, Seedling stage
Plant Breeding and Genetics

Abstract

The initial pot culture study at Department of Plant Breeding and Genetics, Agricultural College and Research Institute, Madurai in rice was conducted to confirm the tolerance mechanism of the donor parents for salinity and submergence separately towards the physiological traits. The parents (ADT 43 and IWP) and donor (FL 478 for salinity and FR 13A for Submergence) were raised in a paper cup maintained separately for salinity and submergence then it was transplanted pots. In each pot, five seedlings were planted and totally fifteen plants were maintained in saline and non-saline condition with three replications. Observations were recorded for all the plants in both saline and non-saline conditions. Regarding for submergence screening, the mud pots along with 14 days old seedlings were drowned in concrete cement tank for 14 days of complete submergence. After 14 days of complete submergence, the samples were collected from each replication both in normal and submergence condition. Hence from the foregoing physiological factors the tolerant donor FL 478 had high level of stable chlorophyll content and also chlorophyll a/b ratio was found unwavering in tolerant donor. Besides the donor parent FR 13A had a ability to retain almost the same level of carbohydrate, starch, non-structural carbohydrate and soluble sugar content under submergence condition. Facts from the study can help rice breeders and other scientists screen and select salinity and submergence tolerant rice breeding lines for variety development and related research, and use the lines identified as tolerant in developing new cultivars.

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Published

2023-03-01

How to Cite

Muthu Vijayaragavan, R., & Murugan, E. (2023). Evaluating Rice for Salinity and Submergence Using Pot-Culture Provides a Systematic Tolerance Assessment at the Seedling Stage. The Indian Journal of Nutrition and Dietetics, 60(1), 128–142. https://doi.org/10.21048/IJND.2023.60.1.29731

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Volume 60, Issue 1, January - March 2023

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Original Articles

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Received 2022-03-05
Accepted 2022-11-24
Published 2023-03-01

 

References

Wang, W., Vinocur, B and Altman, A. Plant responses to drought, salinity and extreme gene that confers submergence tolerance to rice. Nature, 2003, 442, 705-708.

Xu, K., Xia, X., Fukao, T., Canlas, P., Maghirang- Rodriguez, R., Heuer, S., Ismail, A.M., Serres, J.B., Ronald, P.C. and Mackill, D.J. Sub1A is an ethylene response factor-like gene that confers submergence tolerance to rice. Nature, 2006, 442, 705-708. DOI: https://doi.org/10.1038/nature04920

Khanh, T.D., Linh, L.H., Linh, T.H., Ham, L.H. and Xuan, T.D. Rapid and high- precision marker assisted backcrossing to intro gress the SUB1 QTL into the Vietnamese elite rice variety. J. Plant Bred. Crop Sci., 2013, 5, 26-33. DOI: https://doi.org/10.5897/JPBCS12.052

Mackill, D.J., Ismail, A.M. Pamplona, A.M. Sanchez, D.L. Carandang, J.J. and Septiningsih, E.M. Stress tolerant rice varieties for adaptation to a climate change. Crop. Environ. Bioinformatics., 2010, 7, 250-259.

USSL. George E. Brown, Jr Salinity Laboratory. Riverside, CA, USA: USDA-ARS. 2005, http://www.ussl.ars.usda.gov

Koleyoreas, S.A. A new method for determining drought resistance. Plant Physiol., 1958, 33, 232-233. DOI: https://doi.org/10.1104/pp.33.3.232

Sadasivam, S. and Manickam, A. Boichemical Methods. New International (P) Limited. 1996, 06-16, ISBN: 81: 224-0976-8.

Finley, J.W. and Fellers, D.A. Sucrose determination by a modified anthrone method. Application with sweetened wheat-soy blend and corn-soy-milk. Cereal Chem., 1973, 50, 210-215.

Karthikeyan, B., Jaleel, C.A., Gopi, R. and Deiveekasundaram. Alternations in seedling vigor and antioxidant enzyme activities in Catharanthusroseus under seed priming with native diazotrophs. J. Zhejiang University Sci., 2007, 8, 453-457. DOI: https://doi.org/10.1631/jzus.2007.B0453

Jaleel, C.A., Gopi, R., Sankar, B., Manivannan, P., Kishorekumar, A., Sridharan, R. and Panneerselvam. Alternations in germination, seedling vigour lipid peroxidation and proline metabolism in Catharanthusroseus seedling under salt stress. South Afr. J. Botany, 2007, 73, 190-195. DOI: https://doi.org/10.1016/j.sajb.2006.11.001

Manivannan, P., Jaleel, C.A. Kishorekumar, A., Sankar, B., Somasundaram, R., Sridharan, R. and Panneerselvam, R. Propiconazole induced changes in antioxidant metabolism and drought stress amelioration in Vignaun guiculata (L.) Walp, Colloids Surf. B. Biointerface., 2007, 57, 69-74. DOI: https://doi.org/10.1016/j.colsurfb.2007.01.004

Mane, A.V., Karadge, B.A. and Samant, J.S. Salinity induced changes in photosynthetic pigments and polyphenols of Cymbopogon Nardus (L.) Rendle. J. Chem. Pharma. Res., 2010, 2, 338 -347.

Verma, D.P.S. Osmotic stress tolerance in plants: Role of proline and Sulphur metabolism. In. Molecular response to cold, drought, heat and salt stress in higher plants (Eds. Shinozaki, K. and Yamaguchi Shinozaki, K). R.G. lands Company, Texas, USA. 1999, 153-168.

Asch, F., Dingkuhn, M. and Dorffling, K. Salinity increases CO2 assimilation but reduces growth in field-grown, irrigated rice. Plant and Soil, 2000, 218, 1-10. DOI: https://doi.org/10.1023/A:1014953504021

Santo, C.V. Regulation of chlorophyll biosynthesis and degradation by salt stress in sunflower leaves. Sci. Hortic., 2004, 103, 93-99. DOI: https://doi.org/10.1016/j.scienta.2004.04.009

Daiz, M.G.Q., Bebing, N.N. and Villegas, M.J. and Manuel, M.C. Cell biology, Laboratory Manual (Fifth Edition): Genetics and molecular biology division. Philippines: The University of the Philippines, 2002, 47.

Moradi, F. and Ismail, A.M. Responses of photosynthesis, chlorophyll fluorescence and ROS-Scavenging systems to salt stress during seedling and reproductive stages in rice. Ann. Bot., 2007, 99, 1161-1173. DOI: https://doi.org/10.1093/aob/mcm052

Rout, N.P. and Tripathi, S.B. and Shaw, B.P. Effect of salinity on chlorophyll and proline contents in three acquaticmacrophytes. Biol. Plant., 1997, 40, 453-458. DOI: https://doi.org/10.1023/A:1001186502386

Datta, J.K., Nag, S., Banerjee, A. and Mondal, N.K. Impact of salt stress on five varities of wheat (Triticum aestivum L.) cultivars under laboratory condition. J. Appl. Sci. Environ. Manage., 2009, 13, 93-97. DOI: https://doi.org/10.4314/jasem.v13i3.55372

Zhang, Zhen-hua., LIU, Qiang., Song, Hai-xing., Rong, Xiang-min. and Abdelbagi, M. Ismail. Responses of different rice (Oryza sativa L.) genotypes to salt stress and relation to carbohydrate metabolism and chlorophyll content. Afr. J. Agricul. Res., 2012, 7, 19-27. DOI: https://doi.org/10.5897/AJAR11.834

Ferdose, J., Kawasaki, M., Taniguchi, M. and Miyake, H. Differential sensitivity of rice cultivars to salinity and its relation to ion accumulation and root tip structure. Plant Prod Sci., 2009, 12, 453-461. DOI: https://doi.org/10.1626/pps.12.453

Zeng, L., Shannon MC Salinity effects on seedling growth and yield components of rice. Crop Sci., 2000, 40, 996-1003. DOI: https://doi.org/10.2135/cropsci2000.404996x

Saade, S., Maurer, A. and Shahid, M. Yield-related salinity tolerance traits identified in a nested association mapping (NAM) population of wild barley. Sci. Rep., 2016, 6, 32586 DOI: https://doi.org/10.1038/srep32586

Gholizadeh, A., Dehghania, H. and Dvorakb, J. Determination of the most effective traits on wheat yield under saline stress. Agric. Adv., 2014, 3, 103-110.

Lutts, S., Kinet, J.M. and Bouharmont, J. Changes in plant response to NaCl during development of rice (Oryza sativa L.) varieties differing in salinity resistance. J. Exp. Bot., 1995, 46, 1843-1852. DOI: https://doi.org/10.1093/jxb/46.12.1843

Quijano-Guerta, C. and Kirk, G.J.D. Tolerance of rice germ plasm to salinity and other soil chemical stresses in tidal wetlands. Field Crop Res., 2002, 76, 111-121. DOI: https://doi.org/10.1016/S0378-4290(02)00033-3

Panda, D. and Sarkar, R.K. Non structural carbohydrate metabolism associated with submergence tolerance in rice. Genetics. Plant Physiol., 2011, 1, 155-162.

Santiago, R., Barros-Rios, J. and Malvar, R.A. Impact of cell wall composition on maize resistance to pests and diseases. Int. J. Mol. Sci., 2013, 14, 6960-6980. DOI: https://doi.org/10.3390/ijms14046960

Pradhan, B. and Kundagrami, S. Study of relationship between carbohydrate components of stem and survival % by genetic correlation and path analysis for submergence tolerance in rice (Oryza sativa L.). Res. J. Recent Sci., 2016, 5, 2277-2502.