Evaluation of Antibacterial Effect against Pseudomonas aeruginosa and Bacillus subtilis Using Different Solvents of Neolamarckia cadamba Fruit Extracts

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Authors

  • Department of Botany, Ranchi University, Ranchi, Jharkhand - 834 001 ,IN
  • Department of Botany, Ranchi University, Ranchi, Jharkhand - 834 001 ,IN

DOI:

https://doi.org/10.21048/IJND.2023.60.2.31818

Keywords:

Neolamarckia cadamba, fruits, solvents, antibacterial, disc diffusion, ofloxacin

Abstract

Neolamarckia cadamba is a commonly found tree in India and is known for its large green leaves and ball shaped flowers and fruits. Also tree is culturally and economically important plant of India and is traditionally used for preparation of herbal medicines because of its various pharmacological properties. Although bark and leaves are mostly utilized whereas fruits are somewhat lagging behind in terms of its medicinal usages. This present study deals with the antibacterial properties of Neolamarckia cadamba (ripe and unripe) fruits. Six different solvent extracts which includes acetone, benzene, water, DMSO, ethanol and methanol (mg/ml) were prepared for the screening of antibacterial properties of Neolamarckia cadamba fruits against gram negative bacteria Pseudomonas aeruginosa and gram positive bacteria Bacillus subtilis. It was observed that zone of inhibition was highest in ethanolic extract (14 mm) of ripe fruit against the Bacillus subtilis. Zone of inhibition was found lowest in acetonic extract (5 mm) of ripe fruits whereas no zone of inhibition was found in benzene and ethanolic extract of unripe fruits. Out of both fruits, ripe was found to be most effective against both strains whereas out of six solvents ethanolic and methanolic extracts were found to be most effective.

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Published

2023-06-01

How to Cite

Shikha, S., & Kumar, A. (2023). Evaluation of Antibacterial Effect against <i>Pseudomonas aeruginosa</i> and <i>Bacillus subtilis</i> Using Different Solvents of <i>Neolamarckia cadamba</i> Fruit Extracts. The Indian Journal of Nutrition and Dietetics, 60(2), 286–292. https://doi.org/10.21048/IJND.2023.60.2.31818

Issue

Section

Original Articles
Received 2022-10-25
Accepted 2023-06-26
Published 2023-06-01

 

References

Ekor, M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front. Pharmacol., 2014, 177, 1-10. [Google Scholar] DOI: https://doi.org/10.3389/fphar.2013.00177

Pandey, A., Chauhan, A.S., Haware, D.J. and Negi, P.S. Proximate and mineral composition of Kadamba (Neolamarckia cadamba) fruit and its use in the development of nutraceutical enriched beverage. J. Fd. Sci. Technol, 2018, 55, 4330-4336. [Google Scholar] DOI: https://doi.org/10.1007/s13197-018-3382-9

Khwaja, O., Siddiqui, M.H. and Younis, K. Underutilized kadam (Neolamarckia cadamba) fruit: Determination of some engineering properties and drying kinetics. J. Saudi Society of Agricul. Sci., 2020, 19, 401-408. [Google Scholar] DOI: https://doi.org/10.1016/j.jssas.2020.06.001

Ganjewala, D., Tomar, N. and Gupta, A.K. Phytochemical composition and antioxidant properties of methanol extracts of leaves and fruits of Neolamarckia cadamba (Roxb.). J. Biolog. Act. Prod. Nature, 2013, 3, 232-240.[Google Scholar] DOI: https://doi.org/10.1080/22311866.2013.817748

Tairin, I., Abhijit, D., Kumar, B.S., Palash, K., Shafiul, I. and Mohammad, M.S. Evaluation of membrane stabilizing, anthelmintic, antioxidant activity with phytochemical screening of methanolic extract of Neolamarckia cadamba fruits. J. Med. Plants Res., 2015, 9, 151-158. [Google Scholar] DOI: https://doi.org/10.5897/JMPR2014.5720

Shikha, S. and Kumar, A. Comparative Phytochemical Evaluation of the Aerial Parts of Neolamarckia cadamba (Roxb.) Bosser. J. Stress Physiol. Biochem., 2022, 18, 60-68. [Google Scholar]

Sharma, V. and Mathur, G. Phytochemical Evaluation of Anthocephalus Cadamba and In vitro Cytotoxicity Studies. Int. J. Prog. Res. Sci. Engin., 2021, 2, 70-75. [Google Scholar]

Alekhya, V., Deepan, T., Sahoo, S. and Dhanaraju, M.D. Preliminary phytochemical screening and evaluation of in vitro antioxidant activity of Anthocephalous cadamba by using solvent extracts. Euro. J. Biol. Sci., 2013, 5, 34-37.[Google Scholar]

Surani, H.C., Suryawanshi, V.R. and Yadav, H.R. Qualitative and quantitative analysis of Ffruits of Neolamarckia Cadamba (Roxb.), 2022, 1, 1340-1344.[Google Scholar]

Pandey, A. and Negi, P.S. Phytochemical composition, in vitro antioxidant activity and antibacterial mechanisms of Neolamarckia cadamba fruits extracts. Natural prod. Res., 2018, 32, 1189-1192. [Google Scholar] DOI: https://doi.org/10.1080/14786419.2017.1323209

Balouiri, M., Sadiki, M. and Ibnsouda, S.K. Methods for in vitro evaluating antibacterial activity: A review. J. Pharma. Anal., 2016, 6, 71-79.[Google Scholar] DOI: https://doi.org/10.1016/j.jpha.2015.11.005

Naczk, M. and Shahidi, F. Phenolics in cereals, fruits and vegetables: Occurrence, extraction and analysis. J. Pharm. Biomed. Anal., 2006, 41, 1523-1542. [Google Scholar] DOI: https://doi.org/10.1016/j.jpba.2006.04.002

Stalikas, C.D. Extraction, separation, and detection methods for phenolic acids and flavonoids. J. Separate. Sci., 2007, 30, 3268-3295. [Google Scholar] DOI: https://doi.org/10.1002/jssc.200700261

Ghosh, A., Das, B.K., Roy, A., Mandal, B. and Chandra, G. Antibacterial activity of some medicinal plant extracts. J. Natural Med., 2008, 62, 259-262.[Google Scholar] DOI: https://doi.org/10.1007/s11418-007-0216-x

Ahmed, K.B.A., Subramanian, S., Sivasubramanian, A., Veerappan, G. and Veerappan, A. Preparation of gold nanoparticles using Salicornia brachiata plant extract and evaluation of catalytic and antibacterial activity. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2014, 130, 54-58.[Google Scholar] DOI: https://doi.org/10.1016/j.saa.2014.03.070

Gupta, V., George, M., Joseph, L., Singhal, M. and Singh, H.P. Evaluation of antibacterial activity of Bougainvillea glabra ‘snow white’and Bougainvillea glabra ‘choicy’. J. Chem. Pharm. Res., 2009, 1, 233-237.[Google Scholar]

Tian, F., Li, B., Ji, B., Yang, J., Zhang, G., Chen, Y. and Luo, Y. Antioxidant and antibacterial activities of consecutive extracts from Gallachinensis: The polarity affects the bioactivities. Fd. Chem.., 2009, 113, 173-179.[Google Scholar] DOI: https://doi.org/10.1016/j.foodchem.2008.07.062

Hemangi, D. and Ajit, D. Antibacterial activity of Anthoceplalus cadamba and Scirpuskysoorboxb. against food pathogens. Int. J. Curr. Pharm. Res., 2016, 8, 13-18.[Google Scholar] DOI: https://doi.org/10.22159/ijcpr.2016v8i4.15269

Mishra, R.P. Antibacterial properties of Anthocephalus Cadamba fruits. Webmed Central Ayurvedic Med., 2011, 2, 73. [Google Scholar]

Ankenbauer, A., Schäfer, R.A., Viegas, S.C., Pobre, V., Voß, B., Arraiano, C.M. and Takors, R. Pseudomonas putida KT2440 is naturally endowed to withstand industrial scale stress conditions. Microb. Biotechnol., 2020, 13, 1145-1161.[Google Scholar] DOI: https://doi.org/10.1111/1751-7915.13571

Weimer, A., Kohlstedt, M., Volke, D.C., Nikel, P.I. and Wittmann, C. Industrial biotechnology of Pseudomonas putida: Advances and prospects. Appl. Microbiol. Biotechnol., 2020, 104, 7745-7766. [Google Scholar] DOI: https://doi.org/10.1007/s00253-020-10811-9

Haq, A., Siddiqi, M., Batool, S.Z., Islam, A., Khan, A., Khan, D., et al. Comprehensive investigation on the synergistic antibacterial activities of Jatrophacurcas pressed cake and seed oil in combination with antibiotics. Amb. Express, 2019, 9, 1-21.[Google Scholar] DOI: https://doi.org/10.1186/s13568-019-0793-6

Do, Q.D., Angkawijaya, A.E., Tran-Nguyen, P.L., Huynh, L.H., Soetaredjo, F. ., Ismadji, S. and Ju, Y.H. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. J. Fd. Drug Anal., 2014, 22, 296-302. [Google Scholar] DOI: https://doi.org/10.1016/j.jfda.2013.11.001

Sarath, P. A comparative evaluation of phytochemicals in bark, leaves and seeds of Putranjivarox burghii Wall. (Putranjivaceae). J. Pharmacog. Phytochem., 2019, 8, 1162-1166.[Google Scholar]