Momordica charantia Extracts in Selected Media:Screening of Phytochemical Content and In vitro Evaluation of Anti-Diabetic Properties

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Authors

  • Flora-Glad Chizoba Ekezie
     Post Graduate and Research Centre, Department of Foods and Nutrition, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad - 500 030 ,IN
  • W. Jessie Suneetha
     Post Graduate and Research Centre, Department of Foods and Nutrition, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad - 500 030 ,IN
  • K. Uma Maheswari
     Post Graduate and Research Centre, Department of Foods and Nutrition, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad - 500 030 ,IN
  • T. N. V. K. V. Prasad
     Nanotechnology Laboratory, Institute of Frontier Technology, Regional Agricultural Research Station, Acharya N.G. Ranga Agricultural University, Tirupati - 517 502 ,IN
  • B. Anila Kumari
     Post Graduate and Research Centre, Department of Foods and Nutrition, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad - 500 030 ,IN

DOI:

https://doi.org/10.21048/ijnd.2016.53.2.4297

Keywords:

Momordica Charantia, α, -Amylase Activity, A-Glucosidase Activity, Phytochemical Properties, Anti-Diabetic Properties.

Abstract

Diabetes mellitus is a multifunctional disorder which is increasing rapidly in most parts of the world. It has been estimated that up to one third of patients with diabetes mellitus use some form of complementary and alternative medicine. One plant that has received the most attention for its anti-diabetic properties is Momordica charantia commonly referred to as bitter gourd. In this study, bitter gourd was extracted in various media i.e. ethanol, water, citric acid, sodium carbonate and sodium chloride. The samples were evaluated for their in-vitro ability to inhibit α-amylase and α-glucosidase enzymes activity. Ethanol extract was observed to have maximum inhibition against these enzymes. The IC50 values recorded for inhibition of α-amylase activity were 81.34, 87.56, 83.89, 97.71 and 83.12 μg/ml respectively for crude extracts of ethanol, water, citric acid, Na2CO3 and NaCl. Similarly, the ability of extracts to inhibit α-glucosidase was reflected in the result pattern ethanol<Na2CO3<NaCl<aqueous<citric acid (i.e. 72.81<75.66<80.15<81.22<83.85 μg/ml). Therefore, ethanol extract has potential applications in developing drug therapy for the treatment of diabetes using bitter gourd.

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Published

2016-06-01

How to Cite

Ekezie, F.-G. C., Jessie Suneetha, W., Uma Maheswari, K., Prasad, T. N. V. K. V., & Anila Kumari, B. (2016). <I>Momordica charantia</I> Extracts in Selected Media:Screening of Phytochemical Content and <I>In vitro</I> Evaluation of Anti-Diabetic Properties. The Indian Journal of Nutrition and Dietetics, 53(2), 164–171. https://doi.org/10.21048/ijnd.2016.53.2.4297

Issue

Volume 53, Issue 2, April-June 2016

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