Dayak Onions (Eleutherine bulbosa (Mill.) Urb.) Bulbs Extracts Reduce the Blood Pressure of Ovariectomized Rats

Abstract

Background: Eleutherine bulbosa (Mill.) Urb. extract has eleutherinol that could bind with estrogen receptor alpha selectively, therefore it's a candidate for selective estrogen receptor modulator drugs. Objective: To determine the influence of 70% of ethanol extract of E. bulbosa (Mill.) Urb., bulbs on blood pressure of ovariectomized rats. Methods: To analyze the content of the E. bulbosa (Mill.) Urb., bulbs, thin layer chromatography of the extracts were performed using chloroform : methanol (7 : 1, v/v) as the mobile phase. Thirty-six female Sprague-Dawley rats were used for this study, they were divided into six groups: SHAM and ovariectomized both given vehicle; tamoxifen (1.8 mg/kg BW.) as the positive control; three doses of extract at 40, 60, and 90 mg/kg BW were administereted respectively. All groups were ovariectomized on day 1, except the SHAM group. Measurement of blood pressure was performed on the day before ovariectomy, every week after ovariectomy until 28 days before treatment by the extract and then every week after treatment by the extract. Results: Three weeks after treatment of ovariectomized rats by the dose of 90 mg/kg BW of E. bulbosa (Mill.) Urb., extracts, could reduce the systolic blood pressure level to 28.06%, the diastolic to 30.47%. Conclusion: 70% of ethanolic extracts E. bulbosa (Mill.) Urb., could recover the blood pressure of ovariectomized rats, particularly at 90 mg/kg.