The Effect of 70% Ethanolic Extract of Dayak Onion Bulbs and Cowpea on Cardiovascular Parameters of Hypoestrogen Model Rats

Jump To References Section

Authors

  • Anton Bahtiar
     Department Pharmacology and Toxicology, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, West Java ,ID ORCID logo http://orcid.org/0000-0002-2924-3677
  • Farah Amandasari
     Department Pharmacology and Toxicology, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, West Java ,ID

DOI:

https://doi.org/10.18311/jnr/2019/23372

Keywords:

Blood Pressure, Cowpea, Dayak Onion, Hypoestrogen, Lipid Profile.

Abstract

Introduction: Eleutherinol has strong affinity to bind with Estrogen Receptor alpha (ER-α) and can be found in Dayak onion bulbs (Eleutherine bulbosa (Mill.) Urb.). The cowpea (Vigna unguiculata (L.) Walp) contain Daidzein that acts on estrogen receptors beta (ER-β). This study was aimed to investigate the effect of both extracts on ER-α and ER-β by estimating the blood pressure and serum lipid profile of hypoestrogen model rats. Material and Methods: Thirty-six of female Sprague-Dawley were randomly assigned to eight groups as followed, SHAM, OVX, RAL (Raloxifene 1 mg/200 g BW), Cowpea 100 mg/200 g BW with Dayak onion bulbs: 36 mg/200 g BW (D1); 18 mg/200 g BW (D2); 9 mg/200 g BW (D3); 4.5 mg/200 g BW (D4) and single dose of cowpea 100 mg/200 g BW (D5). All groups, except the SHAM,were ovariectomized to obtain the conditions of hypoestrogen. The extracts were given orally for 28 days. Blood pressure and lipid profile were measured after 28 days of treatment. Results: Combination of extracts at D2 (18 mg/200 g BW Dayak onion bulbs and 100 mg/200 g BW cowpea) significantly decreased blood pressure and serum lipid profile of hypoestrogen model rats. The combination extracts was also better than a single dose of 100 mg/200 g BW cowpea. Conclusions: The results indicated that a combination extract of Dayak onion bulbs and cowpea could be used as a drug for menopause woman in the future.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Downloads

Published

2019-06-24

How to Cite

Bahtiar, A., & Amandasari, F. (2019). The Effect of 70% Ethanolic Extract of Dayak Onion Bulbs and Cowpea on Cardiovascular Parameters of Hypoestrogen Model Rats. Journal of Natural Remedies, 19(2), 74–79. https://doi.org/10.18311/jnr/2019/23372

Issue

Volume 19, Issue 2, April 2019

Section

Research Articles
Crossref
Scopus
Google Scholar
Europe PMC
Received 2019-03-04
Accepted 2019-05-21
Published 2019-06-24

 

References

Reece JB, Urry LA, Cain ML, Wasserman SA, Minorsky PV, Jackson RB. Campbell Biology (9th ed.). San Fransisco: Pearson Benjamin Cummings; 2011.

Pfaffl MW, Lange IG, Daxenberger A, Meyer HH. Tissue-specific expression pattern of Estrogen Receptors (ER): Quantification of ER alpha and ER beta mRNA with real-time RT-PCR. APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica. 2001; 109(5): 345–55. PMid: 11478682. https://doi.org/10.1034/j.1600-0463.2001.090503.x

Paterni I, Granchi C, Katzenellenbogen JA, Minutolo F. Estrogen Receptors alpha (ERα) and beta (ERβ): Subtype-selective ligands and clinical potential. Steroids. 2014; 90: 13–29. PMid: 24971815 PMCid: PMC4192010 https://doi.org/10.1016/j.steroids.2014.06.012

Foryst-Ludwig A, Clemenz M, Hohmann S, Hartge M, Sprang C, Frost N, ... Kintscher U. Metabolic actions of Estrogen Receptor beta (ERβ) are mediated by a negative cross-talk with PPARγ. PLoS Genetics. 2008; 4(6). PMid: 18584035 PMCid: PMC2432036. https://doi.org/10.1371/journal.pgen.1000108

da Silva CC, Lazzaretti C, Fontanive T, Dartora DR, Bauereis B, Gamaro GD. Estrogen-dependent effects on behavior, lipid-profile and glycemic index of ovariectomized rats subjected to chronic restraint stress. Behavioural Processes. 2014; 103: 327–33. PMid: 24496020 https://doi.org/10.1016/j.beproc.2014.01.022

Hargrove GM, Junco A, Wong NC. Hormonal regulation of apolipoprotein AI. Journal of Molecular Endocrinology. 1999; 22(2): 103–11. PMid: 10194513. https://doi.org/10.1677/jme.0.0220103

Shen M, Shi H. Sex hormones and their receptors regulate liver energy homeostasis. International Journal of Endocrinology. 2015; 2015: 1–12. PMid: 26491440 PMCid: PMC4600502. https://doi.org/10.1155/2015/294278

Xu B, Lovre D, Mauvais-Jarvis F. Effect of selective Estrogen Receptor modulators on metabolic homeostasis. Biochimie; 2015. p. 1–6. http://www.ncbi.nlm.nih.gov/pubmed/26133657

An KC. Selective Estrogen Receptor modulators. Asian Spine Journal. 2016; 10(4): 787–91. PMid: 27559463 PMCid: PMC4995266. https://doi.org/10.4184/asj.2016.10.4.787

Johnson I, Williamson G. Phytochemical functional foods. New York. Cambridge: Woodhead Publishing Limited; 2003. https://doi.org/10.1201/9780203506318

Bahtiar A, Vichitphan K, Han J. Leguminous plants in the Indonesian Archipelago: Traditional Uses and Secondary Metabolites. Natural Product Communications. 2017; 12(3): 461–472. PMid: 30549910. https://doi.org/10.1177/1934578X1701200338

Bahtiar A, Chumala DY. Dayak onions (Eleutherine bulbosa (Mill.) Urb.) Bulbs extracts reduce the blood pressure of ovariectomized rats. Journal of Natural Remedies. 2018; 36(3): 1016–21. https://doi.org/10.4067/S0717-95022018000301016

Sun MY, Ye Y, Xiao L, Rahman,K, Xia W, Zhang H. Daidzein: A review of pharmacological effects. African Journal of Traditional, Complementary and Alternative Medicines. 2016; 13(3): 117. https://doi.org/10.21010/ajtcam.v13i3.1

Jia M, Dahlman-Wright K, Gustafsson JA. Estrogen R alpha and beta in health and disease. Best Practice and Research: Clinical Endocrinology and Metabolism. 2015; 29(4): 557–68. PMid: 26303083. https://doi.org/10.1016/j.beem.2015.04.008

Pallottini V, Bulzomi P, Galluzzo P, Martini C, Marino M. Estrogen regulation of adipose tissue functions: Involvement of Estrogen Receptor isoforms. Infectious Disorders-Drug Targets (Formerly Current Drug Targets-Infectious Disorders). 2008; 8(1): 52–60. https://doi.org/10.2174/187152608784139631

Rodwell VW, Bender DA, Botham KM, Kennelly PJ, Weil PA. Harper's illustrated biochemistry. Harper''s Illustrated Biochemistry (30th ed.). New York: McGraw-Hill Education LLC; 2015.