Antioxidant Properties of Red Betel (Piper crocatum) Leaf Extract and Its Compounds

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Authors

  • I Nyoman Ehrich Lister
     Faculty of Medicine, Universitas Prima Indonesia, Jl. Belanga No. 1 simp. Ayahanda, Medan –20118, North Sumatera ,ID
  • Chrismis Novalinda Ginting
     Faculty of Medicine, Universitas Prima Indonesia, Jl. Belanga No. 1 simp. Ayahanda, Medan –20118, North Sumatera ,ID
  • Ermi Girsang
     Faculty of Medicine, Universitas Prima Indonesia, Jl. Belanga No. 1 simp. Ayahanda, Medan –20118, North Sumatera ,ID
  • Adek Armansyah
     Faculty of Medicine, Universitas Prima Indonesia, Jl. Belanga No. 1 simp. Ayahanda, Medan –20118, North Sumatera ,ID
  • Hans Hotma Marpaung
     Faculty of Medicine, Universitas Prima Indonesia, Jl. Belanga No. 1 simp. Ayahanda, Medan –20118, North Sumatera ,ID
  • Ade Putra Fratama Sinaga
     Faculty of Medicine, Universitas Prima Indonesia, Jl. Belanga No. 1 simp. Ayahanda, Medan –20118, North Sumatera ,ID
  • Rr. Anisa Siwianti Handayani
     Biomolecular and Biomedical Research Center, Aretha Medika Utama, Jl. Babakan Jeruk II no.9 Bandung – 40163, West Java ,ID
  • Rizal Rizal
     Biomolecular and Biomedical Research Center, Aretha Medika Utama, Jl. Babakan Jeruk II no.9 Bandung – 40163, West Java ,ID

DOI:

https://doi.org/10.18311/jnr/2019/23633

Keywords:

Eugenol, Free Radicals, Hydroxychavicol, Piper crocatum

Abstract

Background: Piper crocatum, also known as red betel, is a potential herbal medicine. Aims: Current study was planned to determine the antioxidant activities of red betel (Piper crocatum Ruiz and Pav.) leaves extract (RBLE) as compared to eugenol and hydroxychavicol compounds. Methods: DPPH radical scavenging, H2O2 scavenging, ABTS reduction, and FRAP reduction assay were carried out. Results: In DPPH scavenging, RBLE showed an IC50 value of 3.98 μg/mL, eugenol of 2.98 μg/mL, and hydroxychavicol of 18.00 μg/mL. Meanwhile, H2O2 scavenging activity showed an IC50 value of RBLE, eugenol, and hydroxychavicol as 186.33 μg/mL, 97.36 μg/mL, and 41.06 μg/mL respectively. ABTS reduction assay showed an IC50 value of 38.43 μg/mL, 181 μg/mL, and 3.10 μg/mL for RBLE, eugenol, and hydroxychavicol respectively. The highest FRAP reduction activity was shown by Eugenol with a concentration of 50 μg/mL which was equal to 424.67 μM Fe (II)/μg. Conclusion: The RBLE and its compounds (eugenol and hydroxychavicol) have antioxidant activity as indicated by the results of the DPPH scavenging, H2O2 scavenging, ABTS reduction, and FRAP reduction assays. However, RBLE had the lowest antioxidant activity compared to other compounds.

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Published

2020-01-03

How to Cite

Nyoman Ehrich Lister, I., Ginting, C. N., Girsang, E., Armansyah, A., Marpaung, H. H., Sinaga, A. P. F., Anisa Siwianti Handayani, R., & Rizal, R. (2020). Antioxidant Properties of Red Betel (<i>Piper crocatum</i>) Leaf Extract and Its Compounds. Journal of Natural Remedies, 19(4), 198–205. https://doi.org/10.18311/jnr/2019/23633

Issue

Volume 19, Issue 4, October 2019

Section

Research Articles
Crossref
Scopus
Google Scholar
Europe PMC
Received 2019-04-29
Accepted 2019-10-14
Published 2020-01-03

 

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