In Vitro Anti-oxidant and Antidiabetic Activity of the Leaves of Stereospermum sauveolens (Roxb.) DC

Abstract

Background: Diabetes mellitus is a metabolic condition characterised by a persistent rise in blood glucose levels. Diabetes is just one of the many disorders that can be treated using natural remedies made from plants. Stereospermum suaveolens (Roxb.) DC, belongs to the family Bignoniaceae, commonly known as Padri. It is employed as a folk remedy in southern India to cure diabetes. Aim: The aim of this study is to assess the potential anti-diabetic and antioxidant properties of the hydroalcoholic extract of S. suaveolens (Roxb.) DC leaves through phytochemical screening and in vitro assays. Methods: Leaves of S. suaveolens (Roxb.) DC were collected and processed to obtain a hydroalcoholic extract. The extract was subjected to qualitative analysis to identify the presence of various phytochemical constituents. The free radical scavenging activity of the extract was evaluated using Nitric oxide radical scavenging assay and DPPH racial scavenging assay. In vitro anti-diabetic studies were performed through α-amylase inhibition assay and glucose uptake assay. Results: The hydroalcoholic extract of S. suaveolens (Roxb.) DC leaves exhibited the presence of bioactive compounds. The extract demonstrated dose-dependent free radical scavenging activity in the in vitro antioxidant assays. The extract showed remarkable inhibitory activity against α-amylase, suggesting its potential in controlling carbohydrate digestion. Also the extract significantly enhanced glucose uptake by yeast cells, indicating its potential to regulate blood glucose levels. Conclusion: The findings of this study indicate that the hydroalcoholic extract of S. suaveolens (Roxb.) DC leaves possesses both antioxidant and anti-diabetic properties. These results suggest its potential as a natural medicinal agent for the management and treatment of type 2 diabetes mellitus.