Evaluation of the Antimicrobial, Antioxidant, and Cytotoxicity Against MCF-7 Breast Cancer Cell Lines of Phyllanthus emblica L. Bark Extract

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Authors

  • Sabari Rani Ganesh Moorthy
     Department of Biotechnology, Faculty of Engineering, Karpagam Academy of Higher Education (Deemed to be University), Pollachi, Eachanari, Coimbatore - 641021, Tamil Nadu ,IN
  • Sasidharan Satheesh Kumar
     Department of Biotechnology, Faculty of Engineering, Karpagam Academy of Higher Education (Deemed to be University), Pollachi, Eachanari, Coimbatore - 641021, Tamil Nadu ,IN
  • Kishore Devandaran
     Department of Biotechnology, Faculty of Engineering, Karpagam Academy of Higher Education (Deemed to be University), Pollachi, Eachanari, Coimbatore - 641021, Tamil Nadu ,IN
  • Santhosh Anguchamy
     Department of Biotechnology, Faculty of Engineering, Karpagam Academy of Higher Education (Deemed to be University), Pollachi, Eachanari, Coimbatore - 641021, Tamil Nadu ,IN
  • Ramachandra Ragunathan
     Department of Biotechnology (Bionanotechnology), Centre for Bioscience and Nanoscience Research, Eachanari, Coimbatore - 641021, Tamil Nadu ,IN
  • Jesteena Johney
     Department of Biotechnology (Bionanotechnology), Centre for Bioscience and Nanoscience Research, Eachanari, Coimbatore - 641021, Tamil Nadu ,IN

DOI:

https://doi.org/10.18311/jnr/2024/36228

Keywords:

Antibacterial, Antioxidant, Total Phenol Content, Cytotoxicity Assay

Abstract

Background: The euphorbiaceous plant family includes Phyllanthus emblica, which demonstrates a variety of pharmacological qualities. Aim: This study aims to investigate any potential medical benefits that the Phyllanthus emblica tree’s bark may have. Methods: An aqueous extract was made with sterile water, and alcohol-based extracts were obtained by macerating with 100% ethanol. Different extraction parameters were changed to evaluate their impact on polyphenol extraction, such as the concentrations of ethanol and aqueous extracts. The phosphomolybdate method was used to assess antioxidant activity and Total Phenol Content (TPC). Both ethanolic and aqueous extracts were found to have antibacterial activity against clinical isolates of Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli. Thin Layer Chromatography (TLC) was used to compare the extracts’ metabolite profiles. The MTT assay was employed to detect cell growth and survival, and the ethanolic bark extract showed potential cytotoxic action against the MCF 7 cancer cell line. Result: The results of the aforementioned tests showed that the ethanolic bark extract had greater antibacterial activity than the aqueous extract. Furthermore, the MTT assay showed that the ethanolic bark extract may have cytotoxic effects on the cancer cell line MCF 7. Conclusion: In summary, more research is necessary to completely understand the antibacterial and chemotherapeutic effects of the active ingredients, opening the door for their possible development as effective anticancer treatments.

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Published

2024-05-01

How to Cite

Ganesh Moorthy, S. R., Kumar, S. S., Devandaran, K., Anguchamy, S., Ragunathan, R., & Johney, J. (2024). Evaluation of the Antimicrobial, Antioxidant, and Cytotoxicity Against MCF-7 Breast Cancer Cell Lines of <i>Phyllanthus emblica</i> L. Bark Extract. Journal of Natural Remedies, 24(5), 1091–1097. https://doi.org/10.18311/jnr/2024/36228

Issue

Volume 24, Issue 5, May 2024

Section

Research Articles

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Copyright (c) 2024 Sabari Rani Ganesh Moorthy, Sasidharan Satheesh Kumar, Kishore Devandaran, Santhosh Anguchamy, Ramachandra Ragunathan, Jesteena Johney (Author)

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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Received 2024-01-16
Accepted 2024-03-21
Published 2024-05-01

 

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