Biofabrication of Silver Nanoparticles Using Green Seaweed: Characterisation and Antibacterial Evaluation

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Authors

  • Nanthakumar Ramalingam
     Sri Ramachandra Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research (Deemed to be University), Porur, Chennai – 600116, Tamil Nadu ,IN
  • Prabakaran Venkatachalam
     Novitium Pharma LLC, East Windsor, New Jersey – 08520 ,US
  • Chellan Rose
     Department of Biotechnology, CSIR – Central Leather Research Institute, Adyar, Chennai – 600020, Tamil Nadu ,IN
  • Seethalakshmi Sankar
     Department of Pharmacology, ESI Medical College and PGIMSR, K. K. Nagar, Chennai – 600012, Tamil Nadu ,IN

DOI:

https://doi.org/10.18311/jnr/2024/36458

Keywords:

Antibacterial Activity, Characterisation, Green Seaweed, Silver Nanoparticles

Abstract

This study investigates the Chaetomorpha antennina (CA) seaweed-mediated biosynthesis of silver nanoparticles (AgNPs). The synthesis process of silver nanoparticles was monitored over time with the help of an Ultraviolet-visible spectrophotometer and further characterisation studies were also performed. Differential Light Scattering (DLS) measurements revealed a mean particle size of approximately 103.5nm and a mean zeta potential value of -57.5mV for AgNPs. The spherical shape and size of the AgNPs were confirmed through High-Resolution Transmission Electron Microscopy (HR-TEM) imaging, while Energy Dispersive X-ray Spectroscopy (EDAX) analysis provided insights into the elemental composition. The concentration of AgNPs was estimated using Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES). The antibacterial potential of AgNPs was evaluated against both gram-positive (Bacillus cereus, Staphylococcus aureus and Bacillus subtilis) and gram-negative bacterial strains (Klebsiella pneumoniae, Escherichia coli, Shigella dysentriae, Salmonella typhi, Pseudomonas aeruginosa and Proteus mirabilis) using the agar well diffusion method. From the results, AgNPs exhibited significant antibacterial activity against B. subtilis and S. typhi among all the tested concentration levels (25, 50, 75 and 100μl).

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Published

2024-04-30

How to Cite

Ramalingam, N., Venkatachalam, P., Rose, C., & Sankar, S. (2024). Biofabrication of Silver Nanoparticles Using Green Seaweed: Characterisation and Antibacterial Evaluation. Journal of Natural Remedies, 24(4), 861–867. https://doi.org/10.18311/jnr/2024/36458

Issue

Volume 24, Issue 4, April 2024

Section

Research Articles

License

Copyright (c) 2024 Nanthakumar Ramalingam, Prabakaran Venkatachalam, Chellan Rose, Seethalakshmi Sankar (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Europe PMC
Received 2024-02-04
Accepted 2024-03-01
Published 2024-04-30

 

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