Evaluation of Antiulcer Activity of Hydroalcohol Extract of Peel of Musa balbisiana Fruits by a New Apparatus and Model

Abstract

Background: Ulcers, a prevalent gastrointestinal disorder, impose significant health burdens worldwide. There are lots of market antiulcer drugs to treat the ulcer , but due to lots of their side effects and high cost, the utility of herbal medicines is increasing day by day. Traditional experimental in vivo approaches involve animal sacrifice, raising ethical concerns, while in vitro models do not show the antiulcer effect directly on the tissue. In response, our study developed a non-invasive model, eliminating the need for animal sacrifice while ensuring reliable results. Aim: This study explored the potential antiulcer properties of Musa balbisiana fruit peel hydroalcohol extract, utilising a novel apparatus and an animal crueltyfree model. Methods: Musa balbisiana fruit peel hydroalcohol extract 400 mg as a test drug and marketed Digene tablets as a standard were assessed using the developed model, and ulcer parameters were evaluated through innovative apparatus measurements. Results: In the blue index analysis, tissue treated with Ringer-Locke’s salt solution, Ringer-Locke’s salt solution + HCl, Ringer-Locke’s salt solution with Digene 400 mg + HCl, Ringer-Locke’s salt solution with M. balbisiana fruit peel hydroalcohol extract 400 mg + HCl showed the blue index of respective values of 52.95, 83.42, 64.48, and 65.51. Conclusion: The research results demonstrated promising antiulcer effects as compared to control and standard when blue indexes are measured, suggesting the potential therapeutic value of M. balbisiana fruit peel in ulcer management. Furthermore, the utilisation of a non-invasive model underscores its applicability in ethical research practices, fostering.