An In-vitro Evaluation of Microwave Irradiation and Denture Cleansers on Disinfection and Destaining of Denture Base Resin

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Authors

  • Sanjay Sharma
     Department of Prosthodontics, Crown & Bridge and Implantology, Modern Dental College and Research Centre, Gandhi Nagar, Airport Road, Indore, MP 453112 ,IN
  • Divya S. Sharma
     Department of Pediatric & Preventive Dentistry, Modern Dental College and Research Centre, Indore (MP) ,IN
  • Umesh Palekar
     Department of Prosthodontics, Crown & Bridge and Implantology, Modern Dental College and Research Centre, Gandhi Nagar, Airport Road, Indore, MP 453112 ,IN

Keywords:

Denture Stomatitis, Denture Staining, Microwave Irradiation
Prosthodontics

Abstract

Purpose: Denture cleansing tablets have been used effectively against stains and Candida when soaked for longer time yet complete sterilization against broad range of bacteria is questionable. Microwave sterilization has been shown effective against bacteria and fungus both but not against stains. Our study aims to comparatively evaluate the efficacy of microwave irradiation and denture cleanser on the disinfection and destaining of stained denture.

Materials and Methods: Ninety-nine acrylic sheets were stained with Acacia catechu and lime and sterilized in UV chamber. Three samples served as negative control and remaining 96 samples were divided in groups A, B, C and D according to contaminating microorganism; three bacteria and one fungus. According to time of irradiation, groups were subdivided into subgroups-1 (2 min), 2(4 min), 3(6 min) and 4 (denture cleanser for 8 h). Microwave irradiation was done either in presence of water or denture cleansing solution. Following procedure samples were scored for staining and then inoculated for 24 h. Then after broth were checked for OD. One sample from each subgroup was inoculated for cfu count.

Results: Effective disinfection for all stained samples achieved with microwave for 6 min in presence of water or denture cleansing solution. Denture cleanser for 8 h was more effective for Candida albicans. 100% stain removal was found with irradiation for 6 min in presence of denture cleanser.

Conclusion: 6 min microwave irradiation in presence of denture cleansing solution could achieve complete sterilization even for spore and membrane forming bacteria. Simultaneously it completely destained all the samples.

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Published

2018-11-06

How to Cite

Sharma, S., Sharma, D. S., & Palekar, U. (2018). An In-vitro Evaluation of Microwave Irradiation and Denture Cleansers on Disinfection and Destaining of Denture Base Resin. Journal of Pierre Fauchard Academy (India Section), 27(1), 32–39. Retrieved from https://informaticsjournals.co.in/index.php/jpfa/article/view/22639

Issue

Volume 27, Issue 1, March 2013

Section

Original Articles

License

All the articles published in JPFA are distributed under a creative commons license. The journal allows the author(s) to hold the copyright of their work (all usages allowed except for commercial purpose).

 

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