Cytomorphometric Analysis of Exfoliative Cytology in Buccal Mucosa of Smokers and Non Smokers in Diabetic Patients in Salem Population – A Comparative Study

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Authors

  • Sanjith K.
     Second Year Post Graduate Students, Department of Oral Pathology, Vinayaka Mission's Sankarachariyar Dental College, Vinayaka Mission's Research Foundation (Deemed to be University), Ariyanur - 636308, Salem, Tamil Nadu ,IN
  • Vishalini L.
     Second Year Post Graduate Students, Department of Oral Pathology, Vinayaka Mission's Sankarachariyar Dental College, Vinayaka Mission's Research Foundation (Deemed to be University), Ariyanur - 636308, Salem, Tamil Nadu ,IN
  • Diana Prem
     Senior Lecturer, Department of Oral Pathology, Vinayaka Mission's Sankarachariyar Dental College, Vinayaka Mission's Research Foundation (Deemed to be University), Ariyanur - 636308, Salem, Tamil Nadu ,IN
  • Ambika Murugesan
     Senior Lecturer, Department of Oral Pathology, Vinayaka Mission's Sankarachariyar Dental College, Vinayaka Mission's Research Foundation (Deemed to be University), Ariyanur - 636308, Salem, Tamil Nadu ,IN
  • Maya Ramesh
     Professor, Department of Oral Pathology, Vinayaka Mission's Sankarachariyar Dental College, Vinayaka Mission's Research Foundation (Deemed to be University), Ariyanur - 636308, Salem, Tamil Nadu ,IN
  • Rajathi Palani
     Reader, Department of Oral Pathology, Vinayaka Mission's Sankarachariyar Dental College, Vinayaka Mission's Research Foundation (Deemed to be University), Ariyanur - 636308, Salem, Tamil Nadu ,IN

DOI:

https://doi.org/10.18311/jpfa/2020/25955

Keywords:

Buccal Mucosa, Cytomorphometric Analysis, Diabetic Patients, H&E Staining, Non-smokers, Oral Exfoliative Cytology, Smokers
Oral Pathology

Abstract

Introduction: Altered cellular microenvironment of oral cavity is a not an uncommon finding in diabetes mellitus patients. Environmental factors also play a pivotal role in cellular changes where tobacco smoking is one of the major risk factor. Aim & Objective: The aim of the study is to find the cytological changes in oral mucosal cells of diabetic people with and without smoking by using cytomorphometric analysis. Materials and Methods: Oral smears were taken from 50 diabetic patients where 25 with smoking habit and 25 without smoking habit from the oral pathology department of Vinayaka mission's Sankarachariyar dental college and hospital. The smears were fixed with 95%ethyl alcohol and stained with hematoxylin and eosin stain. The stained section observed under image analyzer for cytomorphometric analysis. Results: Cellular and nuclear alterations was significantly increased in diabetic patients with smoking habit when compared to diabetic patients without smoking, with a significant P value of 0.001. Conclusion: Diabetes mellitus influences a definite changes in cellular microenvironment in oral mucosa of smokers. However, further research is required to analyze the significance of these findings as a tool for identifying precancerous condition of oral tissues at an early stage.

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Published

2020-12-08

How to Cite

K., S., L., V., Prem, D., Murugesan, A., Ramesh, M., & Palani, R. (2020). Cytomorphometric Analysis of Exfoliative Cytology in Buccal Mucosa of Smokers and Non Smokers in Diabetic Patients in Salem Population – A Comparative Study. Journal of Pierre Fauchard Academy (India Section), 34(4), 107–112. https://doi.org/10.18311/jpfa/2020/25955

Issue

Volume 34, Issue 4, December 2020

Section

Original Articles

License

All the articles published in JPFA are distributed under a creative commons license. The journal allows the author(s) to hold the copyright of their work (all usages allowed except for commercial purpose).

 

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