Estimation of Oral Neutrophil Counts Following Periodontal Therapy in Patients with Chronic Periodontal Disease Utilizing a Standardized Oral Rinse - Interventional Study

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Authors

  • Rani Bhargavan
     Department of Periodontics and Implantology, Annoor Dental College and Hospital, Muvattupuzha - 686673, Kerala ,IN
  • Jose Paul
     Department of Periodontics and Implantology, Annoor Dental College and Hospital, Muvattupuzha - 686673, Kerala ,IN
  • Johnson D’lima
     Department of Periodontics and Implantology, Annoor Dental College and Hospital, Muvattupuzha - 686673, Kerala ,IN
  • Senny Thomas
     Department of Periodontics and Implantology, Annoor Dental College and Hospital, Muvattupuzha - 686673, Kerala ,IN

DOI:

https://doi.org/10.18311/jpfa/2021/26701

Keywords:

Acridine Orange, Fluorescence Microscope, Hemo Cytometer, Oral Neutrophils, Periodontitis

Abstract

Background: Orogranulocytic Migratory Rate (OMR) is defined as the amount of neutrophils move into the oral cavity, through the gingival sulcus. When inflammatory changes occur in the oral cavity, there is an increase in the level of orogranulocytic migratory rate. The oral neutrophil count may be an indicator of the periodontal health status and also it correlates with the severity of periodontal disease. The aim of the study was to assess the relationship of oral neutrophil counts with severity of chronic periodontal diseases and to estimate their levels in response to phase 1 periodontal therapy. Materials and Methods: A total of 66 subjects were selected from the Departments of Periodontics, Annoor Dental College and Hospital, Kerala, India. The subjects were divided into three groups of 22 subjects in each group with two visits. The groups were diagnosed on the basis of gingival index and the number of periodontal pockets. Oral rinse samples of subjects were taken before and 4 weeks after periodontal treatment ((phase 1). Acridine orange was used to stain the cells in the rinse samples. A fluorescence microscope and hemocytometer was used to count neutrophils. Results: There was significant difference in mean value of oral neutrophils before and after treatment for control, moderate periodontitis and severe periodontitis groups. Conclusion: The oral rinse assay was capable to identify a major decrease in oral neutrophil counts in patients who reacted to periodontal treatment (phase 1 therapy). This method is safe, easy, reliable and noninvasive method of quantification of oral neutrophils.

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Published

2022-07-19

How to Cite

Bhargavan, R., Paul, J., D’lima, J., & Thomas, S. (2022). Estimation of Oral Neutrophil Counts Following Periodontal Therapy in Patients with Chronic Periodontal Disease Utilizing a Standardized Oral Rinse - Interventional Study. Journal of Pierre Fauchard Academy (India Section), 35(4), 119–127. https://doi.org/10.18311/jpfa/2021/26701

Issue

Volume 35, Issue 4, December 2021

Section

Original Research Article

License

All the articles published in JPFA are distributed under a creative commons license. The journal allows the author(s) to hold the copyright of their work (all usages allowed except for commercial purpose).

 

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