In Vitro Propagation and Antibacterial Activity of Ocimum basilicum Linn.

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Authors

  • Minu Venugopal
     PG and Research Department of Biotechnology, Kongunadu Arts and Science College, Coimbatore – 641 029, Tamil Nadu ,IN
  • P. Iyappan
     Department of Biotechnology, Muthayammal College of Arts and Science, Rasipuram, Namakkal-637408, Tamil Nadu ,IN
  • R. Subash Kumar
     PG and Research Department of Biotechnology, Kongunadu Arts and Science College, Coimbatore – 641 029, Tamil Nadu ,IN

DOI:

https://doi.org/10.15613/sijrs/2015/v2i1/80082

Keywords:

Antibacterial Activity, MIC, Micropropagation, Ocimum basilicum.

Abstract

The study introduced a simple, reliable procedure for the in vitro culture and standardization of indigenous plant Ocimum basilicum. A potential antibacterial activity was observed in the extracts (chloroform, methanol and petroleum ether) of Ocimum basilicum against selected bacterial strains (MTCC type strains) and their MIC (Minimal Inhibitory Concentration) values were 62.5 and 125 μg mL-1. Existence of tannin, carbohydtares, phenolic compounds, proteins and aminoacids, anthraquinone glycosides, alkaloids were confirmed by phytochemical analysis. In vitro propagation from nodal explants and shoot tip of O. basilicum showed a higher level frequency of micro shoots on MS medium containing plant growth regulators at various combinations and concentrations. The nodal explants produced maximum shooting response (98 %); maximum shoot length (7.9 cm) and maximum multiple shoot formation (n=4.5) in BAP containing medium after 40 days. A 50% concentration of MS medium with combination of IBA and BAP showed maximum ischolar_maining response (78 %), maximum number of ischolar_mains (n=12) and maximum length of ischolar_main (8.67 cm) after 25 days. The regenerates were then hardened off and young plants were transferred to field. Nodal explants in MS medium with 2, 4 - D showed a fast growth and pale brown colored calli.

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Published

2015-06-01

Issue

Volume 2, Issue 1, January-June 2015

Section

Library Science

 

References

Alia B., Nasreen J., Aji A., Saima N. B., Shahida H., Syeda H., “Phytochemical and pharmacological studies on Ocimum basilicum Linn- A Reviewâ€, Int J Cur Res Rev, Vol. 4(23), pp. 73–83, 2012.

Ahmed A., Medine G., Meryem S., Hatice O., Fikrettin S., Isa K., “Antimicrobial effects of Ocimum basilicum (Labiatae) extractâ€, Turk J Biol, Vol. 29, pp. 155–160, 2005.

Chopra R. N., Nayar S. L., Chopra I. C., “Glossary of Indian Medicinal Plants (Including the Supplement)â€, CSIR: New Delhi; 1994.

Faisal M., Shahzad A., Ahmad N., Anis M., Alatar A., Alwathnani A. H., “An efficient system for in vitro multiplication of O.basilicum through node cultureâ€, Afr J Biotechnol, Vol. 11(22), pp. 6055–6059, 2012.

Quamar K. A., Dar A., Siddiqui B. S., “Anticancer activity of Ocimum basilicum and the effect of ursolic acid on the cytoskeleton of MCF-7 human breast cancer cellsâ€, Let in Drug Design and Discov, Vol. 7(10), pp. 726–736, 2010.

Chang S. H., Ho C. K., Tsai J., “Micropropagation of Taxus mairei seedlings at different stages and recoverability of their palgitropic shootsâ€, Taiwan J Sci, Vol. 13, pp. 29–39, 1998.

Pattnaik S., Chand P. K., “In vitro propagation of the medicinalherbs Ocimu’m americanum L. Syn. O. canum Sims. (Hoary basil) and Ocimum sanctum L. (holy basil)â€, Plant Cell Rep, Vol. 15, pp. 846–850, 1996.

Chandrashekar K., Santanu S., Prasanna K.,“ Phytochemical studies of aerial parts of the plant Leucas lavandulaefoliaâ€, Der Pharma Chemica, Vol. 2(5), pp. 434–437, 2010.

Kumar A. P., Kumud U., “Preliminary phytochemical screening and physico-chemical parameters of aerial parts of Artemisia vulgarisâ€, Int J Res Ayur Pharm, Vol. 1(1), pp. 206–211, 2010.

Kokate C. K., Purohit A. P., Gokhale S.B., “Pathway to screen photochemical nature of nature drugsâ€, Pharmacognosy, Vol. 42, pp. A3–A4, 2008.

Jain M. L., Jain S. R., “Therapeutic utility of Ocimum basilicum Linn. var. albumâ€, Planta Med, Vol. 54, pp. 66–70, 1988.

Dhar A. K., “Sweet Basil: Ocimum basilicum- a reviewâ€, J Med Arom Plant Sci, Vol. 24, pp. 738–755, 2002.

Priyakumari I., Sheela V. L., “Micropropagation of Gladiolus cv. ‘Peach blossom’ through enhanced release of axillary budsâ€, J Trop Agri, Vol. 43(1-2), pp. 47–50, 2005.

Najma I., Uzma R., Amla B., “Crucial role of TDZ in the quick regeneration of multiple shoots of Clitoria Ternateaâ€, Int J Pharma Sci Rev Res, Vol. 6(2), pp. 23–26, 2011.

Mercy S., Sangeetha N., Doss G., “Phloroglucinol and silver nitrate enhances axillary shoot proliferation in nodal explants of Vitex negundo L. – an aromatic medicinal plantâ€, Iranian J Biotechnol, Vol. 8(2), pp. 82–89, 2010.

Biju D., Arun K. D., Stalin N., “An efficient protocol for multiple shoot initiation in Coleus vettiveroides Jacob, medicinally important plantâ€, Adv Bio Tech, Vol. 10(09), pp. 34–36, 2011.

Ashok K. J., Mudasir B., “In-vitro propagation of a medicinal plant Portulaca grandiflora. Hookâ€, World J Agri Sci, Vol. 6(3), pp. 327–330, 2010.

Mohamed S. S., Hansi H. P. D., “Antimicrobial activity and phytochemical analysis of selected Indian folk medicinal plantsâ€, Int J Pharma Sci Res, Vol. 1(10), pp. 430–434, 2010.

Babu S., Sathish S., Mohana D. C., Ragavendra M. P., Raveesha K. A., “Antibacterial evaluation and phytochemical analysis of some Iranian medicinal plant pathogenic Xanthomonas pathovarâ€, J Agri Tech, Vol. 3(2), pp. 307–316, 2007.

Mahesh B., Satish S., “Antimicrobial activity of some important medicinal plant against plant human pathogensâ€, World J Agri Sci, Vol. 4, pp. 839–843, 2008.

Sekar J., “Antibacterial activity of plant extracts- Cassia auriculata and Emblica fischeriâ€, Plant Articles, Vol. 10(2), pp. 819–824, 2010.

Prashith K. T. R., Vinayaka K. S., Soumya K. V., Ashwini S. K., Kiran R., “Antibacterial and antifungal activity of methanolic extract of Abrus pulchellus wall and Abrus precatorius Linn. – A comparative studyâ€, Int J Toxicol Pharm Res, Vol. 2(1), pp. 26–29, 2010.

Syed H., Keshava C., Chandrashekar K. R., “Phytochemical evaluation and antibacterial activity of Pterospermum diversifolium blumeâ€, Int J Pharma Sci, Vol. 3(2), pp. 165–167, 2011.

Prakash O. R., Kedar K. R., Rabinarayan A., Sagar K. M., “Preliminary pharmacogonostical and phytochemical evaluation of Coleus aromaticus Benth. Leafâ€, Int J Pharma World Res. Vol. 1(4), pp. 1–19, 2010.

Shanthi M., Swaminathan C., “Evaluation of antibacterial activity and phytochemical analysis of leaves of Withania somnifera (L.) Dunalâ€, Int J Cur Res, Vol. 3(3), pp. 010–012, 2011.