Host-insect and host-plant associated diversity in microbiota isolated from most important Oriental-Australian region egg parasitoid
Authors
-
ICAR-National Bureau of Agricultural Insect Resources, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore – 24, Karnataka ,IN
S. K. Jalali -
ICAR-Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore – 89, Karnataka ,INS. Sriram
-
1ICAR-National Bureau of Agricultural Insect Resources, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore – 24, Karnataka ,INT. Venkatesan
-
1ICAR-National Bureau of Agricultural Insect Resources, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore – 24, Karnataka ,INR. P. More
-
ICAR-National Bureau of Agricultural Insect Resources, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore – 24, Karnataka ,INOmprakash Navik
-
ICAR-National Bureau of Agricultural Insect Resources, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore – 24, Karnataka ,INY. Lalitha
-
ICAR-National Bureau of Agricultural Insect Resources, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore – 24, Karnataka ,INRakshit Ojha
DOI:
https://doi.org/10.18311/jbc/2017/18228Keywords:
Egg parasitoid, evolutionary relationship, host-insects, host-plants, microbiotaAbstract
Host-insect and host-plant associated differentiation of genetically divergent microbiota were recorded from economically important egg parasitoid collected from 26 locations in India, Trichogramma chilonis constituted 86.8% of the populations collected. It was recorded from 14 host-insects, 14 different crops and weed plants from 12 states. Nine species of yeast were recorded from parasitoid from 5 host-insects with Wickerhamomyces anamalus was isolated from 36.4% samples and highest numbers were recorded from parasitoid collected on sugarcane. Bacillus cereus, Pseudomonas sp. and Stenotrophomonas maltophilia from T. chilonis constitute 64.3% of bacterial diversity based on their 16S rDNA sequences. For taxonomic identification using 16s rDNA and ITS sequences, we performed taxonomic classification of total 33 ITS isolates against UNITE Fungal ITS database and assigned taxonomy hierarchy to the sequences. Also, a total of 13 isolates 16s rDNA sequences were taxonomically assigned against RDP 16s rDNA database using RDP Naive Bayesian rRNA Classifier Version 2.1. Most of the species are correctly identified in the respective species members with high confidence threshold value support.
Downloads
Metrics
Downloads
Published
How to Cite
Issue
Section
Accepted 2018-02-01
Published 2018-04-06
References
Bernays EA, Klein BA. 2002. Quantifying the symbiont contribution to essential amino acids in aphids: The importance of tryptophan for Uroleucon ambrosiae. Physiol Entomol. 27: 275–284. Crossref.
Bignell DE. 1984. The arthropod gut as an environment for microorganisms. In: Anderson JM, Rayner ADAM, Walton Exeter DWH (Eds.) Invertebrate-microbial interactions. Cambridge University Press, Cambridge, UK, pp. 205–227.
Bilodeau E, Guay J-F, Turgeon J, Cloutier C. 2013. Survival to parasitoids in an insect hosting defensive symbionts: A multivariate approach to polymorphic traits affecting host use by its natural enemy. PLoS One. 8: e60708. Crossref. PMid:23565269 PMCid:PMC3614562
Bourtzis K, Miller T. 2003. Insect symbiosis. CRC Press, Boca Raton, FL, USA, pp. 324. Crossref. PMCid:PMC1462605
Broderick NA, Raffa KF, Handelsman J. 2006. Midgut bacteria required for Bacillus thuringiensis insecticidal activity. Proc Natl Acad Sci USA. 103: 15196-15199. Crossref. PMid:17005725 PMCid:PMC1622799
Chandler JA, Morgan Lang J, Bhatnagar S, Eisen JA, Kopp A. 2011. Bacterial communities of diverse Drosophila species: Ecological context of a host-microbe model system. PLoS Genet. 7: e1002272. Crossref. PMid:21966276 PMCid:PMC3178584
Colman DR, Toolson EC, Takacs-Vesbach CD. 2012. Do diet and taxonomy influence insect gut bacterial communities? Mol Ecol. 21: 5124–5137. Crossref. PMid:22978555
de Souza DJ, Bézier A, Depoix D, Drezen JM, Lenoir A. 2009. Blochinannia endosymbionts improve colony growth and immune defence in the ant Camponotus fellah. BMC Microbiol. 9: 29. Crossref. PMid:19200360 PMCid:PMC2660346
Deshpande V, Wang Q, Greefield P, Charleston M, PorrasAlfaro A, Kuske CR, Cole JR, Midgley DJ, Tran-Dinh N. 2016. Fungal identification using a Bayesian classifier and the ‘Warcup' training set of internal transcribed spacer sequences. Mycologia 108: 1-5. Crossref. PMid:26553774
Dillon RJ, Dillon VM. 2004. The gut bacteria of insects: Non pathogenic interactions. Annu Rev Entomol. 49: 71–92. Crossref. PMid:14651457
Engel P, Martinsonb VG, Moran NA. 2012. Functional diversity within the simple gut microbiota of the honey bee. Proc Natl Acad Sci USA. 109: 11002–11007. Crossref. PMid:22711827 PMCid:PMC3390884
Ferrari J, Godfray HC, Faulconbridge AS, Via S. 2006. Population differentiation and genetic variation in host choice among PEA aphids from eight host plant genera.
Evolution. 60: 1574–1584. Crossref. PMid:17017058 Ferrari J, Scarborough CL, Godfray HCJ. 2007. Genetic variation in the effect of a facultative symbiont on host-plant use by pea aphids. Oecologia 153: 323–329. Crossref. PMid:17415589
Ferrari J, Vavre F. 2011. Bacterial symbionts in insects or the story of communities affecting communities. Philos Trans R Soc Lond B Biol Sci. 366: 1389–1400. Crossref. PMid:21444313 PMCid:PMC3081568
Gauthier J-P, Outreman Y, Mieuzet L, Simon J-C. 2015. Bacterial communities associated with host-adapted populations of pea aphids revealed by deep sequencing of 16S ribosomal DNA. PLoS One 10: e0120664. Crossref. PMid:25807173 PMCid:PMC4373712
Hagen KS, Tassan RL, Sawall Jr EF. 1970. Some ecophysiological relationships between certain Chrysopa, honeydews and yeasts. Boll Lab Entomol Agrar Filippo Silvestri Portici. 28: 113–134.
Hammer TJ, Dickerson JC, Fierer N. 2015. Evidence-based recommendations on storing and handling specimens for analyses of insect microbiota. Peer J. 18: 3e1190.
Hansen AK, Moran NA. 2014. The impact of microbial symbionts on host plant utilization by herbivorous insects.
Mol Ecol. 23: 1473–1496. Crossref. PMid:23952067 Hassan SA. 1997. Selection of species of Trichogramma for use in biological control programs. In: (Parra JRP, Zucchi RA (eds.) Trichogramma and Applied Biological Control, Piracicaba: ESALQ, Brazil, pp. 183–205. PMid:9272632
Jalali SK, Singh SP. 1993. Superior strain selection of the egg parasitoid Trichogramma chilonis Ishii - Biological parameters. J Biol Control 7: 57-60.
Jalali SK, Singh SP, Venkatesan T, Murthy KS, Lalitha Y. 2006. Development of endosulfan tolerant strain of an egg parasitoid Trichogramma chilonis Ishii (Hymenoptera: Trichogrammatidae). Indian J Exp Biol. 44: 584–590. PMid:16872050
Kaufman MG, Klug MJ. 199.) The contribution of hindgut, bacteria to dietary carbohydrate utilization by crickets (Orthoptera: Gryllidae). Comp Biochem Physiol. 98: 117–123. Crossref.
Lachance MA, Bowles JM. 2002. Metschnikowia arizonensis and Metschnikowia dekortorum, two new large-spore yeast species associated with floricolous beetles. FEMS Yeast Res. 2: 81–86. Crossref.
Leonardo TE, Muiru GT. 2003. Facultative symbionts are associated with host plant specialization in pea aphid populations. Proc R Soc Lond B Biol Sci. 270: 209–212. Crossref. PMid:14667385 PMCid:PMC1809968
McFall-Ngai MJ. 2007. Adaptive immunity: care for the community. Nature 445: 153. Crossref. PMid:17215830
Medina FF, Nachappa P, Tamborindeguy C. 2011. Differences in bacterial diversity of host-associated populations of Phyllexera notabilis Pergande (Hemiptrea: Phylloxeridae) in pecan and water hickory. J Evol Biol. 24: 761–771. Crossref. PMid:21261774
Meyer JM, Hoy MA. 2008. Molecular survey of endosymbionts in Florida populations of Diaphorina citri (Hemiptera: Psyllidae) and its parasitoids Tamarixia radiata (Hymenoptera: Eulophidae) and Diaphorencyrtus aligarhensis (Hymenoptrea: Encyrtidae). Florida Entomol. 91: 294–304. Crossref.
Montagna M, Chouaia B, Mazza G, Prosdocimi EM, Crotti E, Mereghetti V, Vacchini V, Giorgi A, Biase AD, Cervo R, Lozzia GC, Alma A, Bandi C, Daffonchio D. 2015. Effects of the diet on the microbiota of the red palm weevil (Coleoptera: Dryophthoridae). PLoS One 10: e0117439. Crossref. PMid:25635833 PMCid:PMC4311986
Mrázek J, Strosová L, Fliegerová K, Kott T, Kopencí½ J. 2008. Diversity of insect intestinal microflora. Folia Microbiol. 53: 229–233. Crossref. PMid:18661298
Nagarkatti S, Nagaraja H. 1978. Experimental comparison of laboratory reared vs. wild-type Trichogramma confusum (Hym.: Trichogrammatidae) I. Fertility, fecundity and longevity. BioControl 23: 129–136.
Ngo CT, Aujoulat F, Veas F, Jumas-Bilak E, Manguin S. 2015. Bacterial diversity associated with wild caught Anopheles mosquitoes from Dak Nong Province, Vietnam using culture and DNA Fingerprint. PLoS One 10: e0118634. Crossref. PMid:25747513 PMCid:PMC4352016
Nguyen NH, Suh SO, Erbil CK, Blackwell M. 2006. Metschnikowia noctiluminum sp. nov., Metschnikowia corniflorae sp. nov., and Candida chrysomelidae sp. nov., isolated from green lacewings and beetles. Mycol Res. 110: 346–356. Crossref. PMid:16483756
Oliver KM, Moran RL, Hunter MS. 2005. Variation in resistance to parasitism in aphids is due to symbionts not host genotype. Proc Natl Acad Sci USA. 102: 12795–12800. Crossref. PMid:16120675 PMCid:PMC1200300
Priya NG, Ojha A, Kajla MK, Raj A, Raman R. 2012. Host plant induced variation in gut bacteria of Helicoverpa armigera. PLoS One 7, e30768. Crossref. PMid:22292034 PMCid:PMC3266921
Rizzi A, Crotti E, Borruso L, Jucker C, Lupi D, Colombo M, Daffonchio D. 2013. Characterization of the bacterial community associated with larvae and adults of Anoplophora chinensis collected in Italy by culture and culture-independent methods. BioMed Res Int. 2013: 420287. Crossref. PMid:24069601 PMCid:PMC3771249
Simon JC, Carré S, Boutin M, Prunier-Leterme N, SabaterMunoz, Latorre A, Bournoville R. 2003. Host Based divergence in populations of the pea aphid: insights from nuclear markers and the prevalence of facultative symbionts. Proc Biol Sci. 270: 1703-1712. Crossref. PMid:12964998 PMCid:PMC1691435
Srinatha HS, Jalali SK, Sriram S, Chakravarthy AK. 2015. Isolation of microbes associated with field-collected populations of the egg parasitoid, Trichogramma chilonis capable of enhancing biotic fitness. Biocontrol Sci Technol. 25: 789–802. Crossref.
Stireman JO, Nason JD, Heard SB. 2005. Host-associated genetic differentiation in phytophagous insects: General phenomenon or isolated exceptions? Evidence from a goldenrod insect community. Evolution. 59: 2573–2587. Crossref. PMid:16526505
Suh SO, Gibson CM, Blackwell M. 2004. Metschnikowia chrysoperlae sp. nov., Candida picachoensis sp. nov. and Candida pimensis sp. nov., isolated from the green lacewings Chrysoperla comanche and Chrysoperla carnea (Neuroptera: Chrysopidae). Int J Syst Evol Microbiol. 54: 1883–1890. Crossref. PMid:15388758
Tagami Y, Doi M, Sugiyama K, Tatara A, Saito T. 2006. Survey of leafminers and their parasitoids to find endosymbionts for improvement of biological control. Biol Control. 38: 210–216. Crossref.
Toju H, Fukatsu T. 2011. Diversity and infection prevalence of endosymbionts in natural populations of the chestnut weevil: relevance of local climate and host plants. Mol Ecol. 20: 853-868. Crossref. PMid:21199036
Tsuchida T, Koga R, Fukatsu T. 2004. Host plant specialization governed by facultative symbiont. Science 303: 1989. Crossref. PMid:15044797
Vorwerk S, Martinez-Torres D, Forneck A. 2007. Pantoea agglomerans-associated bacteria in grape phylloxera (Daktulosphaira vitifoliae, Fitch). Agric Forest Entomol. 9: 57–64. Crossref.
Wang Q, Garrity GM, Tiedje JM, Cole JR. 2007. Naí¯ve Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol. 73: 5261–5267. Crossref. PMid:17586664 PMCid:PMC1950982
White TJ, Bruns TD, Lee SB, Taylor JW. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (Eds.) PCR Protocols: A guide to Methods and Applications. Academic Press: San Diego, U.S.A., 1990, pp. 315–322. Crossref.
Yun JH, Roh SW, Whon TW, Jung M-J, Kim MS, Park D-S, Yoon C, Nam Y-D, Kim Y-J, Choi J-H, Kim J-Y, Shin N-R, Kim S-H, Lee W-J, Bae J-W. 2014. Insect gut bacterial diversity determined by environmental habitat, diet, developmental stage, and phylogeny of host. Appl Environ Microbiol. 80: 5254-5264. Crossref.
