Bio-efficacy of Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae) against serpentine leafminer, Liriomyza trifoliiBurgess (Diptera: Agromyzidae) in oilseed crops
DOI:
https://doi.org/10.18311/jbc/2019/23030Keywords:
Efficacy, Heterorhabditis bacteriophora, Late instar larva, serpentine leafminerAbstract
Virulence and efficacy of Entomopathogenic Nematode (EPN), Heterorhabditis bacteriophora was tested against late instar larva of serpentine leafminer, Liriomyza trifolii, under laboratory conditions. Five different Infective Juvenile (IJs) concentrations (10, 30, 50, 70 and 100 IJ larva-1) were used. The highest mortality rate caused by H. bacteriophora is 65.1% at 24 hours and 73.8% at 48 hours post inoculation at an inoculation concentration of 100 IJ larva-1. The minimum concentration of 30 IJs per larva shown 37.1% and 52.2% mean larval mortality at 24 and 48 hours post inoculation, respectively. The virulence of H. bacteriophora (LC50) was established at 54 IJs at 24 hours and 37.8 IJs at 48 hours for killing 50% of the larvae tested. The results of this study revealed the potential and scope of EPNs for their utilization in management of L. trifolii in oilseed crops.Downloads
Metrics
Downloads
Published
How to Cite
Issue
Section
References
Chandler LD, Thomas CE. 1983. Seasonal population trends and foliar damage of agromyzid leafminers on cantaloupe in lower Rio Grande Valley, Texas. J Georgia Entomol Soc. 18: 112-120
DOR (Directorate of Oilseeds Research). 1991. Annual Progress Report, 137 pp.
Finney DJ. 1952. Probit Analysis (2nd Ed). J Ins Actuaries 78: 388-390.
Hara A, Kaya K, Gaugler R, LeBeck LM, Mello C.1993. Entomopathogenic nematodes for biological control of the leafminer, Liriomyza trifolii (Agromyzidae). Entomophaga 38: 359–369. https://doi.org/10.1007/ BF02374454
Harris MA, Begley JW, Warkentin DL.1990. Liriomyza trifolii (Diptera: Agromyzidae) suppression with foliar applications of Steinernema carpocapsae (Rhabditida: Steinernematidae) and abamectin. J Econ Entomol. 83: 2380-2384. https://doi.org/10.1093/jee/83.6.2380
Jacob JS, Mathew MP. 2016. Laboratory evaluation of entomopathogenic nematodes against American serpentine leafminer, Liriomyza trifolii (Burgess). J Biopest. 9: 27-33.
Krishnakumar NK. 1998. Bio-ecology and the management of the serpentine leafminer, Liriomyza trifolii on vegetable crops. Final report of ICAR AD-hoc scheme, IIHR, Bangalore
LeBeck L, Gaugler R, Kaya H, Hara H, Johnson W. 1993. Host stage suitability of the leafminer Liriomyza trifolii (Agromyzidae) to the entomopathogenic nematode Steinernema carpocapsae (Rhabditida: Steinernematidae). J Invertebr Pathol. 62: 58–63. https://doi.org/10.1006/jipa.1993.1074
Nguyen NC. 2008. Entomopathogenic nematodes and biological control in Vietnam. Hanoi, Vietnam, Science and Technology Publisher.
Olthof THA, Broadbent AB. 1992. Evaluation of steinernematid nematodes for control of a leafminer, Liriomyza trifolii, in greenhouse chrysanthemums. J Nematol. 24: 612.
Srinivasan K, Virakmath CA, Gupta M, Tewari GC. 1995. Geographical distribution, host range and parasitoids of serpentine leafminer, Liriomyza trifolii (Burgess) in South India. Pest Manage Horti Ecosys. 1: 93-100 White GF. 1927. A method for obtaining infective nematode larvae from culture. Science 66: 302-303. https://doi.org/10.1126/science.66.1709.302-a PMid:17749713
Williams EC, Walters KFA. 2000. Foliar application of the entomopathogenic nematode Steinernema feltiae against leafminers on vegetables. Biocontrol Sci Tech. 10: 61–70. https://doi.org/10.1080/09583150029396
Woodring JL, Kaya HK. 1988. Steinernematid and heterorhabditid nematodes: a handbook of biology and techniques. Southern Cooperative Series Bulletin 331. Fayetteville, AR, USA, Arkansas Agricultural Experiment Station
Zoebisch TC, Schuster DJ, Gilreath JP. 1984. Liriomyza trifolii: Oviposition and development in foliage of tomato and common weed hosts. Fla Entomol. 67: 250-254. https://doi.org/10.2307/3493945