Pathogenicity of Nucleopolyhedrovirus (NPV) against Spodoptera litura (Fabricius)

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Authors

  • M. Kaur
     Department of Microbiology, Punjab Agricultural University, Ludhiana - 141004, Punjab ,IN
  • Neelam Joshi
     Department of Entomology, Punjab Agricultural University, Ludhiana - 141004, Punjab ,IN
  • S. Sharma
     Department of Entomology, Punjab Agricultural University, Ludhiana - 141004, Punjab ,IN
  • A. Kalia
     Department of Soil Sciences, Punjab Agricultural University, Ludhiana - 141004, Punjab ,IN

DOI:

https://doi.org/10.18311/jbc/2021/29342

Keywords:

LC50, LT50, mortality, Nucleopolyhedrosis virus, OBs, Spodoptera litura

Abstract

The management of Spodoptera litura by entomopathogenic Nucleopolyhedrosis Viruses (NPVs) is one of the eco-friendly methods. The present study was aimed to evaluate Nucleopolyhedrosis Virus (NPV) against S. litura and its potential as a bio-pesticide. The Occlusion Bodies (OBs) of S. litura nucleopolyhedrosis virus was isolated from infected larvae collected from cabbage field. Pathogenicity studies evaluated three NPV suspensions viz. SpltNPV-native, SpltNPV-commercial and SpltNPV-NIPHM against second and fourth instar larvae of S. litura recorded maximum mortality at 1×109 OBs/ml. The LC50 values of the SpltNPV-native, SpltNPV-commercial and SpltNPV-NIPHM suspensions against second instar larvae were 0.584, 0.540, 0.625 OBs/mm2, respectively, which increased to 0.696, 0.620, 0.756 OBs/mm2 against the fourth instar larvae. The LT50 at 1×109 OBs/ml was found to increase from 146.33, 137.51 and 155.88 h for SpltNPV-native, SpltNPV-commercial and SpltNPV-NIPHM suspension, respectively, against the second instar larvae to 178.51, 162.07 and 187.67 h, respectively, against the fourth instar larvae. The cumulative per cent mortality, LC50 and LT50 suggested that the second instar larvae were more susceptible and easier to kill than the fourth instar larvae.

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Published

2021-12-01

How to Cite

Kaur, M., Joshi, N., Sharma, S., & Kalia, A. (2021). Pathogenicity of Nucleopolyhedrovirus (NPV) against <i>Spodoptera litura</i> (Fabricius). Journal of Biological Control, 35(4), 218–226. https://doi.org/10.18311/jbc/2021/29342

Issue

Volume 35, Issue 4, December 2021

Section

Research Articles

License

Copyright (c) 2022 Neelam Joshi, Manreet Kaur, Anu Kalia, Sudhendu Sharma

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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Received 2022-01-17
Accepted 2022-07-14
Published 2021-12-01

 

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