Antagonism and molecular identification of Trichoderma isolated from rhizosphere of medicinal plants

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Authors

  • Eder Marques
     Upis Faculdades Integradas, Planaltina-DF ,BR
  • Vanessa Pereira Abreu
     Federal University of Goiás, School of Agronomy, Phytosanitary Department, Phytopathology Research Center, Goiânia - Nova Veneza Highway, Km 0, s/n, 74690-900 ,BR
  • Danilo Rocha de Oliveira
     Upis Faculdades Integradas, Planaltina-DF ,BR
  • Moises Rodrigues Silva
     Federal University of Goiás, School of Agronomy, Phytosanitary Department, Phytopathology Research Center, Goiânia - Nova Veneza Highway, Km 0, s/n, 74690-900 ,BR
  • Flávio Henrique Caetano Santos
     Upis Faculdades Integradas, Planaltina-DF ,BR
  • Keren Hapuque Mendes de Castro
     Federal University of Goiás, School of Agronomy, Phytosanitary Department, Phytopathology Research Center, Goiânia - Nova Veneza Highway, Km 0, s/n, 74690-900 ,BR
  • Marcos Gomes da Cunha
     Federal University of Goiás, School of Agronomy, Phytosanitary Department, Phytopathology Research Center, Goiânia - Nova Veneza Highway, Km 0, s/n, 74690-900 ,BR

DOI:

https://doi.org/10.18311/jbc/2022/30065

Keywords:

Antagonistic fungi, Biological plant disease control, Dual culture, Organic cultivation, Phytopathogenic fungi, Phylogeny., Antagonistic fungi, biological plant disease control, dual culture, organic cultivation, phytopathogenic fungi, phylogeny
Biological control

Abstract

Trichoderma is the most studied and used fungal agent in biological disease control worldwide. Its prospection is a necessary routine, in order to select more effective and specific strains for the different existing agro pathosystems. This work reports the in vitro antagonism (Mycelial Growth Inhibition - MGI) of five Trichoderma isolates, obtained from rhizospheric and organic soil of medicinal plants cultivated in Brazil, to five different phytopathogenic fungi and their molecular identification based on actin (act), calmaldulin (cal), rDNA gene (ITS) and translation elongation factor 1-α (tef1-α). Regarding the fungus Macrophomina phaseolina, the MGI varied between 63.33 and 67.03%; for Fusarium verticillioides between 67.20 and 85.92%; Phaeocytostroma sacchari between 84.00 and 92.90%; in the case of Sclerotinia sclerotiorum, the inhibition was total (100%), and for Sclerotium rolfsii, the antagonism was between 62.03 and 79.07%. According to the molecular phylogeny performed, concatenated analysis of the genetic markers revealed that the five antagonist fungi belong to the Trichoderma afroharzianum species. It is concluded that the T. afroharzianum isolates evaluated showed good levels of in vitro control of the plant pathogenic fungi in question and will be studied via in vivo tests and in plant growth promotion.

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Published

2022-12-14

How to Cite

Marques, E., Abreu, V. P., de Oliveira, D. R., Silva, M. R., Santos, F. H. C., Castro, K. H. M. de, & Cunha, M. G. da. (2022). Antagonism and molecular identification of <i>Trichoderma</i> isolated from rhizosphere of medicinal plants. Journal of Biological Control, 36(1), 07–16. https://doi.org/10.18311/jbc/2022/30065

Issue

Volume 36, Issue 1, March 2022

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Research Articles

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Copyright (c) 2022 Danilo Rocha de Oliveira, Flávio Henrique Caetano Santos, Moises Rodrigues Silva, Keren Hapuque Mendes de Castro, Vanessa Pereira Abreu, Marcos Gomes da Cunha, Eder Marques

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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Received 2022-04-25
Accepted 2022-12-12
Published 2022-12-14

 

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