In vitro assessment of bacterial endophytes for antagonistic activity against Magnaporthe oryzae and Cochliobolus miyabeanus in rice

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Authors

  • ADITYA KUKRETI
     Department of Plant Pathology, University of Agricultural Sciences, GKVK, Bengaluru – 560065, Karnataka ,IN
  • B. S. CHETHANA
     Department of Plant Pathology, University of Agricultural Sciences, GKVK, Bengaluru – 560065, Karnataka ,IN
  • M. K. PRASANNAKUMAR
     Department of Plant Pathology, University of Agricultural Sciences, GKVK, Bengaluru – 560065, Karnataka ,IN
  • C. MANJUNATHA
     Division of Genomic Resources, ICAR-National Bureau of Agricultural Insect Resources, Bengaluru – 560024, Karnataka ,IN
  • NAMBURI KARUNAKAR REDDY
     Department of Plant Pathology, University of Agricultural Sciences, GKVK, Bengaluru – 560065, Karnataka ,IN
  • M. E. PUNEETH
     Department of Plant Pathology, University of Agricultural Sciences, GKVK, Bengaluru – 560065, Karnataka ,IN
  • PARTH GULATI
     Biomaterials Laboratory, Department of Biosciences and Biomedical Engineering, IIT Bhilai, Kutelabhata – 492013, Chhattisgarh ,IN

DOI:

https://doi.org/10.18311/jbc/2023/34946

Keywords:

Biocontrol, dual culture, double petri dish assay, phytopathogens, volatile organic compounds

Abstract

Rice is the global staple food, contributing over half of the world’s agricultural production. Excessive fungicide use in rice farming poses environmental, fungicide resistance, and beneficial microbial disruption concerns, making biocontrol, particularly bacteria, a promising biocontrol agent due to their rapid growth, ease of handling, and robust colonization attributes. Endophytes are endosymbionts residing within host plants, playing a pivotal role in plant health without inciting any pathogenic effects. In this study, we investigated the antagonistic potential of four characterized bacterial endophytic strains, viz., Bacillus velezensis strains A6 and P42, B. pseudomycoides HP3d and Paenibacillus polymyxa PGSS-1 against major foliar rice pathogens viz., Magnaporthe oryzae and Cochliobolus miyabeanus. Against M. oryzae, all the endophytic strains exhibited significant per cent inhibition (50.00-66.67%) and the highest inhibition of 66.67% was achieved by B. pseudomycoides strain HP3d, while the lowest inhibition of 50 and 51.11% was by B. velezensis strain A6, P42, and P. polymyxa PGSS1, respectively. These endophytes outperformed the positive control, P. fluorescens which showed 44.44% inhibition. Similarly, against C. miyabeanus, 31.25-43.75% inhibition was recorded, of which B. velezensis strain A6 exhibited the highest inhibition (43.75%), while B. velezensis strain P42 showed the lowest (31.25%). Furthermore, a double Petri dish assay was conducted to evaluate the volatile compounds produced by these endophytes against the two rice pathogens. It was observed that the volatile compounds produced by B. pseudomycoides strain HP3d and P. polymyxa strain PGSS1 significantly and effectively inhibited the growth of M. oryzae by 88.89% when compared to P. fluorescens (50.00%), whereas B. velezensis strain A6 showed the lowest inhibition (33.33%). Against the brown spot pathogen, C. miyabeanus, the endophytes demonstrated inhibition ranging from 56.25-87.50%, with B. pseudomycoides strain HP3d and P. fluorescens achieving the highest inhibition (87.50%) and B. velezensis strain P42 showing the lowest (56.25%). These results highlight the significant and varied inhibitory effects of volatile compounds released by these endophytes against fungal pathogens of rice. Overall, our findings highlight the promising biocontrol potential of these endophytic strains under in vitro conditions with B. pseudomycoides strain HP3d and P. polymyxa PGSS1 showing exceptional efficacy and they can be deployed in the field for the management of foliar fungal pathogens in rice.

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Author Biography

PARTH GULATI, Biomaterials Laboratory, Department of Biosciences and Biomedical Engineering, IIT Bhilai, Kutelabhata – 492013, Chhattisgarh

 

 

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Published

2023-10-10

How to Cite

KUKRETI, A., CHETHANA, B. S., PRASANNAKUMAR, M. K., MANJUNATHA, C., KARUNAKAR REDDY, N., PUNEETH, M. E., & GULATI, P. (2023). <i>In vitro</i> assessment of bacterial endophytes for antagonistic activity against <i>Magnaporthe oryzae</i> and <i>Cochliobolus miyabeanus</i> in rice. Journal of Biological Control, 37(2), 73–79. https://doi.org/10.18311/jbc/2023/34946

Issue

Volume 37, Issue 2, June 2023

Section

Research Articles

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Copyright (c) 2023 ADITYA KUKRETI, B. S. CHETHANA, M. K. PRASANNAKUMAR, C. MANJUNATHA, NAMBURI KARUNAKAR REDDY, M. E. PUNEETH, PARTH GULATI

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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Received 2023-09-04
Accepted 2023-09-27
Published 2023-10-10

 

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