Assessment of Genetic Variation in Cotesia flavipes Cameron (Hymenoptera: Braconidae) Populations as Revealed by Mitochondrial Cytochrome Oxidase Gene Sequences
Authors
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National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Bangalore 560024, Karnataka ,IN
K. Srinivasa Murthy -
National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Bangalore 560024, Karnataka ,INR. Rajeshwari
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National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Bangalore 560024, Karnataka ,INT. Venkatesan
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National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Bangalore 560024, Karnataka ,ING. Ashok Kumar
DOI:
https://doi.org/10.18311/jbc/2009/3672Keywords:
Cotesia flavipes, Cytochrome Oxidase, Genetic Variation.Abstract
The cytochrome oxidase gene (COI) was employed to assess the genetic variation in different populations of Cotesia flavipes. Partial sequences of the COI gene for the populations from Bangalore, Hyderabad, New Delhi and Shimla were analyzed to assess the homology and the usefulness of this genetic region for phylogenetic studies. PCR using COI-F and COI-R primers amplified a product of approximately 550 bp which was similar for all the four populations. Populations were by and large similar in the COI gene sequenced and there was no variation with our sequences and those of sequences worldwide. The multiple alignments were performed for four populations which revealed similarity of the partial COI gene sequences. Comparative analysis of partial sequences of COI gene produced a phylogenetic tree. Phylogenetic analysis revealed that all our populations were in a single clade with high boot strap value, suggesting highest similarity.
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Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, Z.,Miller, W. and Lipman, D. J. 1997. Gapped BLASTand PSI-BLAST: a new generation of protein database search programmes. Nucleic Acids Research,25: 3389–3402.
Ballal, C. R.., Kumar, P. and Ramani, S. 1995. Laboratoryevaluation, storability and economics of anartificial diet for rearing Chilo partellus (Swinhoe)(Lepidoptera: Pyralidae). Journal of EntomologicalResearch, 19:135–141.
Borah, B. K. and Arya, M. P. S.1995. Natural parasitisationof the sugarcane Plassey borer (Chilo tumidicostalisHmpsn.) by braconid larval parasitoid in Assam. Annals of Agricultural Research, 16 : 362–363.
Dowton, M. and Austin, A. D. 1994. Simultaneousanalysis of 16S, 28S, CO1 and morphology in theHymenoptera: Apocrita-evolutionary transitionsamong parasitic wasps. Proceedings of NationalAcademy of Sciences, 91: 9911–9915.
Fakrudin, B., Prakash, S. H., Krishnareddy, K. B.,Vijaykumar Badari Prasad, P. R., Patil, B. V. andKuruvinashetti, M. S. 2004. Genetic variation ofcotton bollworm, Helicoverpa armigera (Hubner)(Lepidoptera : Noctuidae) of South Indian cottonecosystem using RAPD markers. Current Science,87: 1654–1659.
Felsenstein, J. 1985. Confidence limits on phylogenies:an approach using the bootstrap. Evolution, 39:783–791.
Hoy, M. A. 1994. Insect Molecular Genetics: an Introductionto Principles and Applications. Academic Press,San Diego, CA, USA, 546pp.
Kimura, M. 1980. A simple method for estimatingevolutionary rate of base substitutions throughcomparative studies of nucleotide sequences. Journal of Molecular Evolution, 16: 111–120.
Kfir, R., Overholt, W. A., Khan, Z. R. and Polaszek, A. 2002. Biology and management of economicallyimportant lepidopteran cereal stem borers in Africa. Annual Review of Entomology, 47: 701–731.
Kumar, S., Tamura, K. and Nei, M. 2004. MEGA 3:integrated software for molecular evolutionarygenetics analysis and sequence alignment. Briefings in Bioinformatics, 5: 150–163.
Kankare, M., Stefanescu, C., Saskya, V. N. and Shaw, M. R. 2005. Host specialization by Cotesia wasps(Hymenoptera: Braconidae) parasitizing speciesrich Melitaeini (Lepidoptera: Nymphalidae)communities in north-eastern Spain. BiologicalJournal of the Linnean Society, 86: 45–65.
Mohan, B. R., Verma, A. N. and Singh, S. P. 1991. Periodicparasitisation of Chilo partellus (Swinhoe) larvaeon forage sorghum in Haryana. Journal of InsectScience, 4: 167–169.
Muirhead, K. A., Murphy, N. P., Sallam, M. N., Donnellan,S. C. and Austin, A.D. Mitochondrial DNAphylogeography of the Cotesia flavipes complexof parasitic wasps (Hymenoptera: Braconidae). Annals of the Entomological Society of America,42: 309–318.
Potting, R. P. J.; Vet., L. E. M and Overholt, W. A.1997. Geographic variation in host selection behaviour and reproductive success in the stemborer parasitoid Cotesia flavipes (Hymenoptera:Braconidae). Bulletin of Entomological Research,87: 515–524.
Rattan, R. S., Reineke, A., Ashok, H., Gupta, P. R. andZebitz, C. P. W. 2006. Molecular phylogeny ofCotesia species (Hymenoptera: Braconidae)inferred from a 16s gene. Current Science, 91:1460–1461.
Scott, K. D., Wilkinson, K. S. and Merritt, M. A. 2003. Genetic shifts in Helicoverpa armigera (Hubner)(Lepidoptera: Noctuidae) over a year in theDawson/Callide valleys. Australian Journal ofAgricultural Research, 54: 739–744.
Whitfield, J. B. 1997. Hierarchical analysis of variationin the mitochondrial 16S gene amongHymenoptera. Molecular Biology and Evolution, 15: 1728-1743.
