Growth Characteristics and Bio-Efficacy of Different Isolates of Metarhizium anisopliae (Metschnikoff) Sorokin against Certain Key Insect Pests
Authors
-
Agricultural Research Station, Malnoor 585215, Gulbarga District, Karnataka ,IN
V. Rachappa -
Department of Entomology, University of Agricultural Sciences, Dharwad 580005, Karnataka ,INS. Lingappa
-
Department of Entomology, University of Agricultural Sciences, Dharwad 580005, Karnataka ,INR. K. Patil
DOI:
https://doi.org/10.18311/jbc/2009/3675Keywords:
Bio-Efficacy, Biological Characters, Helicoverpa armigera, Metarhizium anisopliae, Oryctes rhinoceros, Plutella xylostella.Abstract
An attempt was made to know the variation in field collected isolates of Metarhizium anisopliae (Metschnikoff) Sorokin under laboratory conditions. Isolates of M. anisopliae collected from different geographical regions were studied for various parameters like growth and sporulation on potato dextrose agar (PDA) medium and rice based medium, and exhibited considerable variation. Ma3 and Ma4 isolates took 6 days to initiate sporulation while Ma1 and Ma2 isolates took 2 to 3 days on PDA. Mycelial growth in Ma1 and Ma2 isolates, being on par, was significantly higher (38.60 and 42.30 mm, respectively) than the other two isolates (Ma3-32.30 mm and Ma4- 33.19 mm). Ma1 and Ma2 isolates grew faster on rice based medium and yielded conidial yield of 8.93 and 9.32 g per 100 g of medium respectively. Isolate Ma2 was the most virulent against Helicoverpa armigera (Hübner) and Plutella xylostella (Linnaeus) with less LC50 values of 1.77 x 106 and 1.98 x 106 conidia per ml followed by Ma1, which was more virulent against Oryctes rhinoceros (Linnaeus). The lethal time (LT50) was low in Ma2 for H. armigera and P. xylostella (126.15 h and 69.74 h, respectively) in contrast to Ma1 that recorded lower LT50 value against O. rhinoceros (294.47h). The results indicate that Ma2 was the best isolate against lepidopteran insects and Ma1 was the best against coleopterans.
Downloads
Metrics
Downloads
Published
How to Cite
Issue
Section
References
Abbott, W. S. 1925. A method of computing the effectivenessof an insecticide. Journal of EconomicEntomology, 18: 265–267.
Fernando, L. C. P., Kanagaratnam, P. and Narangoda, N. K.1995. Some studies on the use of Metarhiziumanisopliae (Metsch.) Sor. for the control ofOryctes rhinoceros in Sri Lanka. COCOS,10: 46–52.
Ferron, P. and Roberts, P. H. 1975. Virulence ofentomopathogenic fungi for the adults ofAcanthoscelides obtectus (Coleoptera:Bruchidae). Journal of Invertebrate Pathology,25: 379–388.
Ferron, P., Roberts, P. H. and Deotte, A. 1975. Susceptibilityof Oryctes rhinoceros adults to Metarhiziumanisopliae. Journal of Invertebrate Pathology,25: 313–319.
Finney, D. J. 1971. Probit Analysis (3rd Edition). CambridgeUniversity Press, London, 333p.
Milner, R. J. and Prior, C. 1994. Susceptibility of theAustralian plague locust, Chortoicetesterminifera and the wingless grasshopper,Phaulacridium vittatum to the fungi Metarhiziumspp. Biological Control, 4: 132–137.
Milner, R. J., Samson, P. R. and Bullard, G. K. 2002.FI-1045: A profile of a commercially usefulisolate of Metarhizium anisopliae var.anisopliae. Biocontrol Science and Technology,12: 43–58.
Padnad, M. S. 2002. Molecular characterization of nativeentomopathogenic fungi. M. Sc. (Agri.) Thesis,University of Agricultural Sciences, Dharwad,78p.
Roberts, D. W. 1989. World picture of biological control ofinsects by fungi. Memoria del Instituto OswaldoCruz, Rio de Janeiro, 84: 89-100.
Rosa, R. W. Godinez, A. J. L. and Alatorre. R. R.,1995, Biological activity of five strains ofMetarhizium anisopliae upon the coffee berryborer, Hypothenemus hampei. Entomophaga,40: 403–412.
Samuels, K. D. Z. 1986. Genetical studies and strainselection in Metarhizium anisopliae(Metschnikoff) Sorokin for the control ofNilaparvata lugens (Stal.) the brown planthopperof rice. Ph. D. Thesis, University of London,U.K.
Theunis, W. and Aloali, I. I. 1998. Selection of a highlyvirulent fungal isolate, Metarhizium anisopliaeMaTB101 for control of taro beetle, Papuanauninodis (Coleoptera : Scarabaeidae). BiocontrolScience and Technology, 8: 187–195.
