Isolation of Streptomyces from Tobacco Soils that Show Antimicrobial Activity
DOI:
https://doi.org/10.18311/jbc/2009/3697Keywords:
Antibiotics, Antimicrobial Activity, Growth Pattern, Streptomyces, Tobacco Soils.Abstract
Twelve isolates of Streptomyces spp. from tobacco soils were screened for the production of antibiotics, of which two were found to be efficient. Growth pattern of the two potential isolates was studied and their antimicrobial activity was tested against Aspergillus flavus, A. niger, Pythium aphanidermatum, Bacillus subtilis and Pseudomonas fluorescens. Isolate 2 was found to be more effective and caused maximum inhibitory zone of 108 mm after 120 hrs against P. aphanidermatum. The area of the inhibitory zone observed in both the isolates was directly proportional to the age of the culture for up to 120h.Downloads
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Alexander, M. 1961. Introduction to Soil Microbiology. John Wiley and Sons., Inc., New York.
Arai, T. 1976. Clarification of actinomycetes with referenceto antibiotic production, pp. 543–638. In:Actinomycetes: The boundary microorganisms. Toppan Co Ltd., Tokyo, Japan.
Alexander, S.I. and Strete, D. 2001. Filter paper discmethod, pp.102–104. In: Microbiology: Aphotographic atlas for the laboratory. Addison-Wesley Longman, Inc., New York, USA.
Lechevalier, H.A. 1964. Principles and application in aquaticmicrobiology, pp. 230–250. In: Heukelekan andDondero (Eds.), The Actinomycetes. John Wileyand Sons, New York.
Lechevalier, H.A. and Lechevalier, M.P. 1967. Biologyof the actinomycetes. Annual Review ofMicrobiology, 21: 71–100.
Locci, R. 1989. Streptomycetes and related genera, pp. 2451–2469. In: Bergey's manual of systematicBacteriology, Vol. 4 (Williams ST edition). Williams and Willkins, London.
Narayana, K. J. P., Ravi Kiran, D. and Vijayalakshmi, M. 2004. Production of antibiotics from Streptomycesspecies isolated from virgin soils. Indian Journalof Microbiology, 44: 147–148.
Pirous, T., Karbasian, M. A. and Goodfellow, M. 1999. Isolation of some aerobic actinomycetes speciesfrom the soil of Zahedan country, South Eastof Iran. Iran Journal of Medical Sciences,24: 65–67.
Pridham, T. G. and Lyons, A. J. 1980. Methodologiesfor Actinomycetes with special reference toStreptomyces and Streptoverticella, pp.169-170. In: Dietz, A. and Thayer, D. W. (Eds.) Actinomycetetaxonomy. SIM Special publication member 6.
Siva Saba Ratnam and Traquair, J. A. 2000. Formulation of aStreptomyces biocontrol agent for the suppressionof Rhizoctonia damping-off in tomato transplants. Biological Control, 23: 245–253.
Tsao, P. H., Labben, C. and Keitt, G. W. 1960. An enrichmentmethod for isolating Actinomycetes that producediffusible antifungal antibiotics. Phytopathology,50: 80–89.
Waksman, S. A. and Woodruff, H. B. 1940. The soil assource of microorganisms antagonistic to diseaseproducing bacteria. Journal of Bacteriology,40: 581–600.
Williams, S. T. and Cross, T. 1971. Isolation, purification,cultivation and preservation of actinomycetes. Methods in Microbiology, 4: 295–334.
Williams, S. T. and Davis, F. L. 1965. Use of antibioticsfor selective isolation and enumeration ofactinomycetes in soil. Journal of GeneralMicrobiology, 38: 251–262.